tetano
Editor, Senior Moderator
Sci Rep. 2019 Feb 22;9(1):2617. doi: 10.1038/s41598-019-38885-0.
[h=1]Heterosubtypic cross-protection correlates with cross-reactive interferon-gamma-secreting lymphocytes in the ferret model of influenza.[/h] Gooch KE[SUP]1[/SUP], Marriott AC[SUP]2[/SUP], Ryan KA[SUP]1[/SUP], Yeates P[SUP]1[/SUP], Slack GS[SUP]1[/SUP], Brown PJ[SUP]1[/SUP], Fothergill R[SUP]1[/SUP], Whittaker CJ[SUP]1[/SUP], Carroll MW[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] An effective universal vaccine for influenza will likely need to induce virus-specific T-cells, which are the major mediator of heterosubtypic cross-protection between different subtypes of influenza A virus. In this study we characterise the cell-mediated immune response in ferrets during heterosubtypic protection induced by low-dose H1N1 virus infection against an H3N2 virus challenge, given 4 weeks later. Although the ferrets were not protected against the infection by H3N2 virus, the duration of virus shedding was shortened, and clinical disease was markedly reduced. No cross-reactive neutralizing antibodies were detected, but cross-reactive interferon-gamma-secreting T cells were detected in the circulation prior to H3N2 challenge. These T-cells peaked at 11 days post-H1N1 infection, and were strongly induced in blood and in lung following H3N2 infection. The rapid induction of interferon-gamma-secreting cells in ferrets previously infected with H1N1 virus, but not in na?ve ferrets, suggests induction of memory T-cells. These results are in accord with the observations that pre-existing cross-reactive T-cells correlate with protection in humans and have implications for outbreak modelling and universal vaccine design.
PMID: 30796267 DOI: 10.1038/s41598-019-38885-0
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[h=1]Heterosubtypic cross-protection correlates with cross-reactive interferon-gamma-secreting lymphocytes in the ferret model of influenza.[/h] Gooch KE[SUP]1[/SUP], Marriott AC[SUP]2[/SUP], Ryan KA[SUP]1[/SUP], Yeates P[SUP]1[/SUP], Slack GS[SUP]1[/SUP], Brown PJ[SUP]1[/SUP], Fothergill R[SUP]1[/SUP], Whittaker CJ[SUP]1[/SUP], Carroll MW[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] An effective universal vaccine for influenza will likely need to induce virus-specific T-cells, which are the major mediator of heterosubtypic cross-protection between different subtypes of influenza A virus. In this study we characterise the cell-mediated immune response in ferrets during heterosubtypic protection induced by low-dose H1N1 virus infection against an H3N2 virus challenge, given 4 weeks later. Although the ferrets were not protected against the infection by H3N2 virus, the duration of virus shedding was shortened, and clinical disease was markedly reduced. No cross-reactive neutralizing antibodies were detected, but cross-reactive interferon-gamma-secreting T cells were detected in the circulation prior to H3N2 challenge. These T-cells peaked at 11 days post-H1N1 infection, and were strongly induced in blood and in lung following H3N2 infection. The rapid induction of interferon-gamma-secreting cells in ferrets previously infected with H1N1 virus, but not in na?ve ferrets, suggests induction of memory T-cells. These results are in accord with the observations that pre-existing cross-reactive T-cells correlate with protection in humans and have implications for outbreak modelling and universal vaccine design.
PMID: 30796267 DOI: 10.1038/s41598-019-38885-0
Free full text