tetano
Editor, Senior Moderator
J Virol Methods. 2013 Nov 8. pii: S0166-0934(13)00449-7. doi: 10.1016/j.jviromet.2013.10.035. [Epub ahead of print]
High-yield soluble expression of recombinant influenza virus antigens from Escherichia coli and their potential uses in diagnosis.
Jang YH, Cho SH, Son A, Lee YH, Lee J, Lee KH, Seong BL.
Source
Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea.
Abstract
Although antiviral drugs and vaccines have been successful for mitigating influenza virus infections, the lack of general technical platform for the timely supply of soluble and highly purified influenza viral antigens presents a serious bottleneck for the subsequent analysis for the effective control of the viral disease. Using the Escherichia coli (E.coli) lysyl tRNA synthetase (LysRS) as a novel fusion partner, this study reports the soluble expression of influenza viral proteins in E.coli host, construction of antibody library against the virus, and detection of anti-influenza antibodies using the expressed viral antigens. When influenza A and B viral proteins were fused with the LysRS, the fusion proteins were expressed predominantly as soluble forms and their production yields were high enough to be amenable to immunization protocols in rabbits for antibody generation. The produced antibodies showed high level binding specificity against the respective viral proteins, with cross-reactivity across heterologous viruses within the same type of influenza viruses. In addition, LysRS-HA fusion protein could bind specifically to anti-HA antibodies in the virus-infected mouse serum in widely accepted two detection methods, Western blot and ELISA. These results present a convenient tool for the production of antibodies specific to the virus as well as the rapid detection of influenza viral infections, ultimately contributing to the control of influenza viruses including highly pathogenic H5N1, pandemic H1N1, or the recent H7N9 influenza viruses.
Copyright ? 2013. Published by Elsevier B.V.
KEYWORDS:
Antibody library, Bacteria, ELISA., Fusion partner, Influenza viruses, Recombinant proteins
PMID:
24216235
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24216235
High-yield soluble expression of recombinant influenza virus antigens from Escherichia coli and their potential uses in diagnosis.
Jang YH, Cho SH, Son A, Lee YH, Lee J, Lee KH, Seong BL.
Source
Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea.
Abstract
Although antiviral drugs and vaccines have been successful for mitigating influenza virus infections, the lack of general technical platform for the timely supply of soluble and highly purified influenza viral antigens presents a serious bottleneck for the subsequent analysis for the effective control of the viral disease. Using the Escherichia coli (E.coli) lysyl tRNA synthetase (LysRS) as a novel fusion partner, this study reports the soluble expression of influenza viral proteins in E.coli host, construction of antibody library against the virus, and detection of anti-influenza antibodies using the expressed viral antigens. When influenza A and B viral proteins were fused with the LysRS, the fusion proteins were expressed predominantly as soluble forms and their production yields were high enough to be amenable to immunization protocols in rabbits for antibody generation. The produced antibodies showed high level binding specificity against the respective viral proteins, with cross-reactivity across heterologous viruses within the same type of influenza viruses. In addition, LysRS-HA fusion protein could bind specifically to anti-HA antibodies in the virus-infected mouse serum in widely accepted two detection methods, Western blot and ELISA. These results present a convenient tool for the production of antibodies specific to the virus as well as the rapid detection of influenza viral infections, ultimately contributing to the control of influenza viruses including highly pathogenic H5N1, pandemic H1N1, or the recent H7N9 influenza viruses.
Copyright ? 2013. Published by Elsevier B.V.
KEYWORDS:
Antibody library, Bacteria, ELISA., Fusion partner, Influenza viruses, Recombinant proteins
PMID:
24216235
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24216235