tetano
Editor, Senior Moderator
Clin Immunol. 2020 Apr 21:108440. doi: 10.1016/j.clim.2020.108440. [Epub ahead of print]
Higher PIK3C2B gene expression of H1N1+ specific B-cells is associated with lower H1N1 immunogenicity after trivalent influenza vaccination in HIV infected children.
Cotugno N[SUP]1[/SUP], Zicari S[SUP]1[/SUP], Morrocchi E[SUP]2[/SUP], de Armas LR[SUP]3[/SUP], Pallikkuth S[SUP]3[/SUP], Rinaldi S[SUP]4[/SUP], Ruggiero A[SUP]1[/SUP], Manno E[SUP]1[/SUP], Zangari P[SUP]1[/SUP], Chiriaco M[SUP]1[/SUP], Bernardi S[SUP]1[/SUP], Andrews SF[SUP]5[/SUP], Cagigi A[SUP]5[/SUP], Rossi P[SUP]1[/SUP], McDermott AB[SUP]5[/SUP], Pahwa S[SUP]3[/SUP], Palma P[SUP]6[/SUP].
Author information
Abstract
Perinatally HIV-infected children (PHIV), despite successful antiretroviral therapy, present suboptimal responses to vaccinations compared to healthy-controls (HC). Here we investigated phenotypic and transcriptional signatures of H1N1-specific B-cells (H1N1-Sp) in PHIV, differentially responding to trivalent-influenza-vaccine (TIV), and HC. Patients were categorized in responders (R) and non-responders (NR) according to hemagglutination-inhibition-assay at baseline and 21 days after TIV. No differences in H1N1-Sp frequencies were found between groups. H1N1-Sp transcriptional analysis revealed a distinct signature between PHIV and HC. NR presented higher PIK3C2B and NOD2 expression compared to R, confirmed by downregulation of PIK3C2B in resting-memory of R after H1N1 in-vitro stimulation. In conclusion this study confirms that qualitative rather than quantitative analyses are needed to characterize immune responses in PHIV. These results further suggest that higher PIK3C2B in H1N1-Sp of NR is associated with lower H1N1 immunogenicity and may be targeted by future modulating strategies to improve TIV responses in PHIV.
Copyright ? 2020. Published by Elsevier Inc.
KEYWORDS:
Antigen specific B cells; Gene expression; H1N1; Influenza vaccination; PIK3C2B; Perinatally HIV infected children
PMID:32330555DOI:10.1016/j.clim.2020.108440
Higher PIK3C2B gene expression of H1N1+ specific B-cells is associated with lower H1N1 immunogenicity after trivalent influenza vaccination in HIV infected children.
Cotugno N[SUP]1[/SUP], Zicari S[SUP]1[/SUP], Morrocchi E[SUP]2[/SUP], de Armas LR[SUP]3[/SUP], Pallikkuth S[SUP]3[/SUP], Rinaldi S[SUP]4[/SUP], Ruggiero A[SUP]1[/SUP], Manno E[SUP]1[/SUP], Zangari P[SUP]1[/SUP], Chiriaco M[SUP]1[/SUP], Bernardi S[SUP]1[/SUP], Andrews SF[SUP]5[/SUP], Cagigi A[SUP]5[/SUP], Rossi P[SUP]1[/SUP], McDermott AB[SUP]5[/SUP], Pahwa S[SUP]3[/SUP], Palma P[SUP]6[/SUP].
Author information
Abstract
Perinatally HIV-infected children (PHIV), despite successful antiretroviral therapy, present suboptimal responses to vaccinations compared to healthy-controls (HC). Here we investigated phenotypic and transcriptional signatures of H1N1-specific B-cells (H1N1-Sp) in PHIV, differentially responding to trivalent-influenza-vaccine (TIV), and HC. Patients were categorized in responders (R) and non-responders (NR) according to hemagglutination-inhibition-assay at baseline and 21 days after TIV. No differences in H1N1-Sp frequencies were found between groups. H1N1-Sp transcriptional analysis revealed a distinct signature between PHIV and HC. NR presented higher PIK3C2B and NOD2 expression compared to R, confirmed by downregulation of PIK3C2B in resting-memory of R after H1N1 in-vitro stimulation. In conclusion this study confirms that qualitative rather than quantitative analyses are needed to characterize immune responses in PHIV. These results further suggest that higher PIK3C2B in H1N1-Sp of NR is associated with lower H1N1 immunogenicity and may be targeted by future modulating strategies to improve TIV responses in PHIV.
Copyright ? 2020. Published by Elsevier Inc.
KEYWORDS:
Antigen specific B cells; Gene expression; H1N1; Influenza vaccination; PIK3C2B; Perinatally HIV infected children
PMID:32330555DOI:10.1016/j.clim.2020.108440