tetano
Editor, Senior Moderator
HLA
. 2025 Jan;105(1):e70011.
doi: 10.1111/tan.70011. Exploring the Relationship Between Humoral and Cellular T Cell Responses Against SARS-CoV-2 in Exposed Individuals From Emilia Romagna Region and COVID-19 Severity
Lucia Mazzotti[SUP] 1 [/SUP], Patricia Borges de Souza[SUP] 1 [/SUP], Irene Azzali[SUP] 1 [/SUP], Davide Angeli[SUP] 1 [/SUP], Oriana Nanni[SUP] 1 [/SUP], Vittorio Sambri[SUP] 2 3 [/SUP], Simona Semprini[SUP] 2 [/SUP], Sara Bravaccini[SUP] 4 [/SUP], Claudio Cerchione[SUP] 1 [/SUP], Anna Gaimari[SUP] 1 [/SUP], Fabio Nicolini[SUP] 1 [/SUP], Valentina Ancarani[SUP] 1 [/SUP], Giovanni Martinelli[SUP] 5 [/SUP], Anna Pasetto[SUP] 6 7 [/SUP], Hugo Calderon[SUP] 8 [/SUP], Manel Juan[SUP] 8 [/SUP], Massimiliano Mazza[SUP] 1 [/SUP]
Affiliations
COVID-19 remains a significant global health problem with uncertain long-term consequences for convalescents. We investigated the relationships between anti-N protein antibody levels, severe acute respiratory syndrome (SARS)-CoV-2-associated TCR repertoire parameters, HLA type and epidemiological information from three cohorts of 524 SARS-CoV-2-infected subjects subgrouped in acute phase, seronegative and seropositive convalescents from the Emilia Romagna region. Epidemiological information and anti-N antibody index were associated with TCR repertoire data. HLA type was inferred from TCR repertoire using the HLA3 tool and its association with clonal breadth (CB) and clonal depth (CD) was assessed. Age above 58 years, male and COVID-19 hospitalisation were significantly and independently associated with seropositivity (p = 0.004; p = 0.004; p = 0.04), suggesting an association between high antibody titres and symptoms' severity. As for the TCR repertoire analysis, we found no difference in CB among the cohorts, while CD was higher in seronegative than acute (p = 0.04). However, clustering analysis supported that seronegative patients are endowed with broader CB and deeper CD indicating a compensatory mechanism without effective seroconversion. The CD calculated on the TCRs associated with the single SARS-CoV-2 ORFs in convalescents is higher when compared to the acute. Lastly, we identified and reported on novel HLAs significantly associated with increased risk of hospitalisation such as HLA-C*07:02 carriers (OR = 3.9, CI = 1.1-13.4, p = 0.03) and on HLAs that associate significantly with lower or higher TCR repertoire parameters in a population exposed for the first time to SARS-CoV-2.
Keywords: COVID‐19; HLA; SARS‐CoV‐2; TCR; immunity; repertoire; severity.
. 2025 Jan;105(1):e70011.
doi: 10.1111/tan.70011. Exploring the Relationship Between Humoral and Cellular T Cell Responses Against SARS-CoV-2 in Exposed Individuals From Emilia Romagna Region and COVID-19 Severity
Lucia Mazzotti[SUP] 1 [/SUP], Patricia Borges de Souza[SUP] 1 [/SUP], Irene Azzali[SUP] 1 [/SUP], Davide Angeli[SUP] 1 [/SUP], Oriana Nanni[SUP] 1 [/SUP], Vittorio Sambri[SUP] 2 3 [/SUP], Simona Semprini[SUP] 2 [/SUP], Sara Bravaccini[SUP] 4 [/SUP], Claudio Cerchione[SUP] 1 [/SUP], Anna Gaimari[SUP] 1 [/SUP], Fabio Nicolini[SUP] 1 [/SUP], Valentina Ancarani[SUP] 1 [/SUP], Giovanni Martinelli[SUP] 5 [/SUP], Anna Pasetto[SUP] 6 7 [/SUP], Hugo Calderon[SUP] 8 [/SUP], Manel Juan[SUP] 8 [/SUP], Massimiliano Mazza[SUP] 1 [/SUP]
Affiliations
- PMID: 39807702
- PMCID: PMC11731316
- DOI: 10.1111/tan.70011
COVID-19 remains a significant global health problem with uncertain long-term consequences for convalescents. We investigated the relationships between anti-N protein antibody levels, severe acute respiratory syndrome (SARS)-CoV-2-associated TCR repertoire parameters, HLA type and epidemiological information from three cohorts of 524 SARS-CoV-2-infected subjects subgrouped in acute phase, seronegative and seropositive convalescents from the Emilia Romagna region. Epidemiological information and anti-N antibody index were associated with TCR repertoire data. HLA type was inferred from TCR repertoire using the HLA3 tool and its association with clonal breadth (CB) and clonal depth (CD) was assessed. Age above 58 years, male and COVID-19 hospitalisation were significantly and independently associated with seropositivity (p = 0.004; p = 0.004; p = 0.04), suggesting an association between high antibody titres and symptoms' severity. As for the TCR repertoire analysis, we found no difference in CB among the cohorts, while CD was higher in seronegative than acute (p = 0.04). However, clustering analysis supported that seronegative patients are endowed with broader CB and deeper CD indicating a compensatory mechanism without effective seroconversion. The CD calculated on the TCRs associated with the single SARS-CoV-2 ORFs in convalescents is higher when compared to the acute. Lastly, we identified and reported on novel HLAs significantly associated with increased risk of hospitalisation such as HLA-C*07:02 carriers (OR = 3.9, CI = 1.1-13.4, p = 0.03) and on HLAs that associate significantly with lower or higher TCR repertoire parameters in a population exposed for the first time to SARS-CoV-2.
Keywords: COVID‐19; HLA; SARS‐CoV‐2; TCR; immunity; repertoire; severity.