tetano
Editor, Senior Moderator
Hum Genomics
. 2022 Jul 27;16(1):27.
doi: 10.1186/s40246-022-00401-3.
Epigenetics may characterize asymptomatic COVID-19 infection
Cosby G Arnold[SUP] 1 [/SUP], Iain Konigsberg[SUP] 2 [/SUP], Jason Y Adams[SUP] 3 [/SUP], Sunita Sharma[SUP] 4 [/SUP], Neil Aggarwal[SUP] 4 [/SUP], Andrew Hopkinson[SUP] 5 [/SUP], Alexis Vest[SUP] 5 [/SUP], Monica Campbell[SUP] 2 [/SUP], Meher Boorgula[SUP] 2 [/SUP], Ivana Yang[SUP] #[/SUP][SUP] 2 [/SUP], Christopher Gignoux[SUP] #[/SUP][SUP] 2 [/SUP], Kathleen C Barnes[SUP] #[/SUP][SUP] 2 [/SUP], Andrew A Monte[SUP] 5 [/SUP]
Affiliations
Abstract
RT-PCR is the foremost clinical test for diagnosis of COVID-19. Unfortunately, PCR-based testing has limitations and may not result in a positive test early in the course of infection before symptoms develop. Enveloped RNA viruses, such as coronaviruses, alter peripheral blood methylation and DNA methylation signatures may characterize asymptomatic versus symptomatic infection. We used Illumina's Infinium MethylationEPIC BeadChip array to profile peripheral blood samples from 164 patients who tested positive for SARS-CoV-2 by RT-PCR, of whom 8 had no symptoms. Epigenome-wide association analysis identified 10 methylation sites associated with infection and a quantile-quantile plot showed little inflation. These preliminary results suggest that differences in methylation patterns may distinguish asymptomatic from symptomatic infection.
Keywords: COVID-19; Epigenetics; Methylation; SARS-CoV-2.
. 2022 Jul 27;16(1):27.
doi: 10.1186/s40246-022-00401-3.
Epigenetics may characterize asymptomatic COVID-19 infection
Cosby G Arnold[SUP] 1 [/SUP], Iain Konigsberg[SUP] 2 [/SUP], Jason Y Adams[SUP] 3 [/SUP], Sunita Sharma[SUP] 4 [/SUP], Neil Aggarwal[SUP] 4 [/SUP], Andrew Hopkinson[SUP] 5 [/SUP], Alexis Vest[SUP] 5 [/SUP], Monica Campbell[SUP] 2 [/SUP], Meher Boorgula[SUP] 2 [/SUP], Ivana Yang[SUP] #[/SUP][SUP] 2 [/SUP], Christopher Gignoux[SUP] #[/SUP][SUP] 2 [/SUP], Kathleen C Barnes[SUP] #[/SUP][SUP] 2 [/SUP], Andrew A Monte[SUP] 5 [/SUP]
Affiliations
- PMID: 35897116
- DOI: 10.1186/s40246-022-00401-3
Abstract
RT-PCR is the foremost clinical test for diagnosis of COVID-19. Unfortunately, PCR-based testing has limitations and may not result in a positive test early in the course of infection before symptoms develop. Enveloped RNA viruses, such as coronaviruses, alter peripheral blood methylation and DNA methylation signatures may characterize asymptomatic versus symptomatic infection. We used Illumina's Infinium MethylationEPIC BeadChip array to profile peripheral blood samples from 164 patients who tested positive for SARS-CoV-2 by RT-PCR, of whom 8 had no symptoms. Epigenome-wide association analysis identified 10 methylation sites associated with infection and a quantile-quantile plot showed little inflation. These preliminary results suggest that differences in methylation patterns may distinguish asymptomatic from symptomatic infection.
Keywords: COVID-19; Epigenetics; Methylation; SARS-CoV-2.