tetano
Editor, Senior Moderator
Hum Vaccin Immunother
. 2024 Dec 31;20(1):2292381.
doi: 10.1080/21645515.2023.2292381. Epub 2024 Jan 9. DNA and protein-generated chimeric molecules for delivery of influenza viral epitopes in mouse and humanized NSG transfer models
Nikolina M Mihaylova[SUP] 1 [/SUP], Iliyan K Manoylov[SUP] 1 [/SUP], Maria H Nikolova[SUP] 2 [/SUP], Jozsef Prechl[SUP] 3 [/SUP], Andrey I Tchorbanov[SUP] 1 4 [/SUP]
Affiliations
Purified subunit viral antigens are weakly immunogenic and stimulate only the antibody but not the T cell-mediated immune response. An alternative approach to inducing protective immunity with small viral peptides may be the targeting of viral epitopes to immunocompetent cells by DNA and protein-engineered vaccines. This review will focus on DNA and protein-generated chimeric molecules carrying engineered fragments specific for activating cell surface co-receptors for inducing protective antiviral immunity. Adjuvanted protein-based vaccine or DNA constructs encoding simultaneously T- and B-cell peptide epitopes from influenza viral hemagglutinin, and scFvs specific for costimulatory immune cell receptors may induce a significant increase of anti-influenza antibody levels and strong CTL activity against virus-infected cells in a manner that mimics the natural infection. Here we summarize the development of several DNA and protein chimeric constructs carrying influenza virus HA317-41 fragment. The generated engineered molecules were used for immunization in intact murine and experimentally humanized NSG mouse models.
Keywords: DNA vaccines; chimeric molecules; engineered antibodies; influenza virus.
. 2024 Dec 31;20(1):2292381.
doi: 10.1080/21645515.2023.2292381. Epub 2024 Jan 9. DNA and protein-generated chimeric molecules for delivery of influenza viral epitopes in mouse and humanized NSG transfer models
Nikolina M Mihaylova[SUP] 1 [/SUP], Iliyan K Manoylov[SUP] 1 [/SUP], Maria H Nikolova[SUP] 2 [/SUP], Jozsef Prechl[SUP] 3 [/SUP], Andrey I Tchorbanov[SUP] 1 4 [/SUP]
Affiliations
- PMID: 38193304
- DOI: 10.1080/21645515.2023.2292381
Purified subunit viral antigens are weakly immunogenic and stimulate only the antibody but not the T cell-mediated immune response. An alternative approach to inducing protective immunity with small viral peptides may be the targeting of viral epitopes to immunocompetent cells by DNA and protein-engineered vaccines. This review will focus on DNA and protein-generated chimeric molecules carrying engineered fragments specific for activating cell surface co-receptors for inducing protective antiviral immunity. Adjuvanted protein-based vaccine or DNA constructs encoding simultaneously T- and B-cell peptide epitopes from influenza viral hemagglutinin, and scFvs specific for costimulatory immune cell receptors may induce a significant increase of anti-influenza antibody levels and strong CTL activity against virus-infected cells in a manner that mimics the natural infection. Here we summarize the development of several DNA and protein chimeric constructs carrying influenza virus HA317-41 fragment. The generated engineered molecules were used for immunization in intact murine and experimentally humanized NSG mouse models.
Keywords: DNA vaccines; chimeric molecules; engineered antibodies; influenza virus.