tetano
Editor, Senior Moderator
J Med Virol. 2015 May 12. doi: 10.1002/jmv.24231. [Epub ahead of print]
[h=1]Human infection with an avian influenza A (H9N2) virus in the middle region of China.[/h] Huang Y[SUP]1[/SUP], Li X[SUP]2[/SUP], Zhang H[SUP]1[/SUP], Chen B[SUP]3[/SUP], Jiang Y[SUP]3[/SUP], Yang L[SUP]2[/SUP], Zhu W[SUP]2[/SUP], Hu S[SUP]1[/SUP], Zhou S[SUP]1[/SUP], Tang Y[SUP]3[/SUP], Xiang X[SUP]1[/SUP], Li F[SUP]1[/SUP], Li W[SUP]1[/SUP], Gao L[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] During the epidemic period of the novel H7N9 viruses, an influenza A (H9N2) virus was isolated from a 7-year-old boy with influenza-like illness in Yongzhou city of Hunan province in November 2013. To identify the possible source of infection, environmental specimens collected from local live poultry markets epidemiologically linked to the human case in Yongzhou city were tested for influenza type A and its subtypes H5, H7, and H9 using real-time RT-PCR methods as well as virus isolation, and four other H9N2 viruses were isolated. The real-time RT-PCR results showed that the environment was highly contaminated with avian influenza H9 subtype viruses (18.0%). Sequencing analyses revealed that the virus isolated from the patient, which was highly similar (98.5-99.8%) to one of isolates from environment in complete genome sequences, was of avian origin. Based on phylogenetic and antigenic analyses, it belonged to genotype S and Y280 lineage. In addition, the virus exhibited high homology (95.7-99.5%) of all six internal gene lineages with the novel H7N9 and H10N8 viruses which caused epidemic and endemic in China. Meanwhile, it carried several mammalian adapted molecular residues including Q226L in HA protein, L13P in PB1 protein, K356R, S409N in PA protein, V15I in M1 protein, I28V, L55F in M2 protein, and E227K in NS protein. These findings reinforce the significance of continuous surveillance of H9N2 influenza viruses. J. Med. Virol. 9999: 1-8, 2015. ? 2015 Wiley Periodicals, Inc.
? 2015 Wiley Periodicals, Inc.
[h=4]KEYWORDS:[/h] H9N2; avian influenza virus; human infection; phylogenetic analysis
PMID: 25965534 [PubMed - as supplied by publisher]
[h=1]Human infection with an avian influenza A (H9N2) virus in the middle region of China.[/h] Huang Y[SUP]1[/SUP], Li X[SUP]2[/SUP], Zhang H[SUP]1[/SUP], Chen B[SUP]3[/SUP], Jiang Y[SUP]3[/SUP], Yang L[SUP]2[/SUP], Zhu W[SUP]2[/SUP], Hu S[SUP]1[/SUP], Zhou S[SUP]1[/SUP], Tang Y[SUP]3[/SUP], Xiang X[SUP]1[/SUP], Li F[SUP]1[/SUP], Li W[SUP]1[/SUP], Gao L[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] During the epidemic period of the novel H7N9 viruses, an influenza A (H9N2) virus was isolated from a 7-year-old boy with influenza-like illness in Yongzhou city of Hunan province in November 2013. To identify the possible source of infection, environmental specimens collected from local live poultry markets epidemiologically linked to the human case in Yongzhou city were tested for influenza type A and its subtypes H5, H7, and H9 using real-time RT-PCR methods as well as virus isolation, and four other H9N2 viruses were isolated. The real-time RT-PCR results showed that the environment was highly contaminated with avian influenza H9 subtype viruses (18.0%). Sequencing analyses revealed that the virus isolated from the patient, which was highly similar (98.5-99.8%) to one of isolates from environment in complete genome sequences, was of avian origin. Based on phylogenetic and antigenic analyses, it belonged to genotype S and Y280 lineage. In addition, the virus exhibited high homology (95.7-99.5%) of all six internal gene lineages with the novel H7N9 and H10N8 viruses which caused epidemic and endemic in China. Meanwhile, it carried several mammalian adapted molecular residues including Q226L in HA protein, L13P in PB1 protein, K356R, S409N in PA protein, V15I in M1 protein, I28V, L55F in M2 protein, and E227K in NS protein. These findings reinforce the significance of continuous surveillance of H9N2 influenza viruses. J. Med. Virol. 9999: 1-8, 2015. ? 2015 Wiley Periodicals, Inc.
? 2015 Wiley Periodicals, Inc.
[h=4]KEYWORDS:[/h] H9N2; avian influenza virus; human infection; phylogenetic analysis
PMID: 25965534 [PubMed - as supplied by publisher]