tetano
Editor, Senior Moderator
Cell Host Microbe. 2014 Jun 25. pii: S1931-3128(14)00184-X. doi: 10.1016/j.chom.2014.05.013. [Epub ahead of print]
Human Responses to Influenza Vaccination Show Seroconversion Signatures and Convergent Antibody Rearrangements.
Jackson KJ1, Liu Y2, Roskin KM3, Glanville J3, Hoh RA3, Seo K3, Marshall EL4, Gurley TC5, Moody MA5, Haynes BF5, Walter EB5, Liao HX5, Albrecht RA6, Garc?a-Sastre A7, Chaparro-Riggers J8, Rajpal A8, Pons J8, Simen BB9, Hanczaruk B9, Dekker CL10, Laserson J11, Koller D11, Davis MM12, Fire AZ13, Boyd SD14.
Author information
Abstract
B cells produce a diverse antibody repertoire by undergoing gene rearrangements. Pathogen exposure induces the clonal expansion of B cells expressing antibodies that can bind the infectious agent. To assess human B cell responses to trivalent seasonal influenza and monovalent pandemic H1N1 vaccination, we sequenced gene rearrangements encoding the immunoglobulin heavy chain, a major determinant of epitope recognition. The magnitude of B cell clonal expansions correlates with an individual's secreted antibody response to the vaccine, and the expanded clones are enriched with those expressing influenza-specific monoclonal antibodies. Additionally, B cell responses to pandemic influenza H1N1 vaccination and infection in different people show a prominent family of convergent antibody heavy chain gene rearrangements specific to influenza antigens. These results indicate that microbes can induce specific signatures of immunoglobulin gene rearrangements and that pathogen exposure can potentially be assessed from B cell repertoires.
Copyright ? 2014 Elsevier Inc. All rights reserved.
PMID:
24981332
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24981332
Human Responses to Influenza Vaccination Show Seroconversion Signatures and Convergent Antibody Rearrangements.
Jackson KJ1, Liu Y2, Roskin KM3, Glanville J3, Hoh RA3, Seo K3, Marshall EL4, Gurley TC5, Moody MA5, Haynes BF5, Walter EB5, Liao HX5, Albrecht RA6, Garc?a-Sastre A7, Chaparro-Riggers J8, Rajpal A8, Pons J8, Simen BB9, Hanczaruk B9, Dekker CL10, Laserson J11, Koller D11, Davis MM12, Fire AZ13, Boyd SD14.
Author information
Abstract
B cells produce a diverse antibody repertoire by undergoing gene rearrangements. Pathogen exposure induces the clonal expansion of B cells expressing antibodies that can bind the infectious agent. To assess human B cell responses to trivalent seasonal influenza and monovalent pandemic H1N1 vaccination, we sequenced gene rearrangements encoding the immunoglobulin heavy chain, a major determinant of epitope recognition. The magnitude of B cell clonal expansions correlates with an individual's secreted antibody response to the vaccine, and the expanded clones are enriched with those expressing influenza-specific monoclonal antibodies. Additionally, B cell responses to pandemic influenza H1N1 vaccination and infection in different people show a prominent family of convergent antibody heavy chain gene rearrangements specific to influenza antigens. These results indicate that microbes can induce specific signatures of immunoglobulin gene rearrangements and that pathogen exposure can potentially be assessed from B cell repertoires.
Copyright ? 2014 Elsevier Inc. All rights reserved.
PMID:
24981332
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24981332