tetano
Editor, Senior Moderator
Zoonoses Public Health. 2012 Sep 17. doi: 10.1111/zph.12006. [Epub ahead of print]
Identification and Analysis of the First 2009 Pandemic H1N1 Influenza Virus from U.S. Feral Swine.
Clavijo A, Nikooienejad A, Esfahani MS, Metz RP, Schwartz S, Atashpaz-Gargari E, Deliberto TJ, Lutman MW, Pedersen K, Bazan LR, Koster LG, Jenkins-Moore M, Swenson SL, Zhang M, Beckham T, Johnson CD, Bounpheng M.
Source
Texas Veterinary Medical Diagnostic Laboratory, College Station, TX, USA Texas AgriLife Research, College Station, TX, USA United States Department of Agriculture, Fort Collins, CO, USA United States Department of Agriculture, San Diego, TX, USA National Veterinary Services Laboratories, Ames, IA, USA.
Abstract
The first case of pandemic H1N1 influenza (pH1N1) virus in feral swine in the United States was identified in Texas through the United States Department of Agriculture (USDA) Wildlife Services' surveillance program. Two samples were identified as pandemic influenza by reverse transcriptase quantitative PCR (RT-qPCR). Full-genome Sanger sequencing of all eight influenza segments was performed. In addition, Illumina deep sequencing of the original diagnostic samples and their respective virus isolation cultures were performed to assess the feasibility of using an unbiased whole-genome linear target amplification method and multiple sample sequencing in a single Illumina GAIIx lane. Identical sequences were obtained using both techniques. Phylogenetic analysis indicated that all gene segments belonged to the pH1N1 (2009) lineage. In conclusion, we have identified the first pH1N1 isolate in feral swine in the United States and have demonstrated the use of an easy unbiased linear amplification method for deep sequencing of multiple samples.
? 2012 Blackwell Verlag GmbH.
PMID:
22978260
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22978260
Identification and Analysis of the First 2009 Pandemic H1N1 Influenza Virus from U.S. Feral Swine.
Clavijo A, Nikooienejad A, Esfahani MS, Metz RP, Schwartz S, Atashpaz-Gargari E, Deliberto TJ, Lutman MW, Pedersen K, Bazan LR, Koster LG, Jenkins-Moore M, Swenson SL, Zhang M, Beckham T, Johnson CD, Bounpheng M.
Source
Texas Veterinary Medical Diagnostic Laboratory, College Station, TX, USA Texas AgriLife Research, College Station, TX, USA United States Department of Agriculture, Fort Collins, CO, USA United States Department of Agriculture, San Diego, TX, USA National Veterinary Services Laboratories, Ames, IA, USA.
Abstract
The first case of pandemic H1N1 influenza (pH1N1) virus in feral swine in the United States was identified in Texas through the United States Department of Agriculture (USDA) Wildlife Services' surveillance program. Two samples were identified as pandemic influenza by reverse transcriptase quantitative PCR (RT-qPCR). Full-genome Sanger sequencing of all eight influenza segments was performed. In addition, Illumina deep sequencing of the original diagnostic samples and their respective virus isolation cultures were performed to assess the feasibility of using an unbiased whole-genome linear target amplification method and multiple sample sequencing in a single Illumina GAIIx lane. Identical sequences were obtained using both techniques. Phylogenetic analysis indicated that all gene segments belonged to the pH1N1 (2009) lineage. In conclusion, we have identified the first pH1N1 isolate in feral swine in the United States and have demonstrated the use of an easy unbiased linear amplification method for deep sequencing of multiple samples.
? 2012 Blackwell Verlag GmbH.
PMID:
22978260
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22978260