tetano
Editor, Senior Moderator
Vaccine. 2018 May 31. pii: S0264-410X(18)30752-7. doi: 10.1016/j.vaccine.2018.05.086. [Epub ahead of print]
[h=1]Identification of human vaccinees that possess antibodies targeting the egg-adapted hemagglutinin receptor binding site of an H1N1 influenza vaccine strain.[/h] Garretson TA[SUP]1[/SUP], Petrie JG[SUP]2[/SUP], Martin ET[SUP]2[/SUP], Monto AS[SUP]2[/SUP], Hensley SE[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Human influenza viruses passaged in eggs often acquire mutations in the hemagglutinin (HA) receptor binding site (RBS). To determine if egg-adapted H1N1 vaccines commonly elicit antibodies targeting the egg-adapted RBS of HA, we completed hemagglutinin-inhibition assays with A/California/7/2009 HA and egg-adapted A/California/7/2009-X-179A HA using sera collected from 159 humans vaccinated with seasonal influenza vaccines during the 2015-16 season. We found that ∼5% of participants had ≥4-fold higher antibody titers to the egg-adapted viral strain compared to wild type viral strain. We used reverse-genetics to demonstrate that a single egg-adapted HA RBS mutation (Q226R) was responsible for this phenotype.
[h=4]KEYWORDS:[/h] Hemagglutinin; Influenza; Vaccines
PMID: 29861178 DOI: 10.1016/j.vaccine.2018.05.086
[h=1]Identification of human vaccinees that possess antibodies targeting the egg-adapted hemagglutinin receptor binding site of an H1N1 influenza vaccine strain.[/h] Garretson TA[SUP]1[/SUP], Petrie JG[SUP]2[/SUP], Martin ET[SUP]2[/SUP], Monto AS[SUP]2[/SUP], Hensley SE[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Human influenza viruses passaged in eggs often acquire mutations in the hemagglutinin (HA) receptor binding site (RBS). To determine if egg-adapted H1N1 vaccines commonly elicit antibodies targeting the egg-adapted RBS of HA, we completed hemagglutinin-inhibition assays with A/California/7/2009 HA and egg-adapted A/California/7/2009-X-179A HA using sera collected from 159 humans vaccinated with seasonal influenza vaccines during the 2015-16 season. We found that ∼5% of participants had ≥4-fold higher antibody titers to the egg-adapted viral strain compared to wild type viral strain. We used reverse-genetics to demonstrate that a single egg-adapted HA RBS mutation (Q226R) was responsible for this phenotype.
[h=4]KEYWORDS:[/h] Hemagglutinin; Influenza; Vaccines
PMID: 29861178 DOI: 10.1016/j.vaccine.2018.05.086