tetano
Editor, Senior Moderator
J Vet Sci. 2018 Jan 25. [Epub ahead of print]
[h=1]Identification, sequence analysis, and infectivity of H9N2 avian influenza viruses isolated from geese.[/h] Zhu R[SUP]1,[/SUP][SUP]2[/SUP], Yang X[SUP]1,[/SUP][SUP]2[/SUP], Zhang J[SUP]3[/SUP], Xu D[SUP]1,[/SUP][SUP]2[/SUP], Fan J[SUP]1,[/SUP][SUP]2[/SUP], Shi H[SUP]1,[/SUP][SUP]2[/SUP], Wang S[SUP]4[/SUP], Liu X[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] H9N2 subtype avian influenza virus is a great threat to the Chinese poultry industry, even with annual vaccination. Waterfowl can be asymptomatically infected with the H9N2 virus. In this study, three strains of the H9N2 avian influenza virus, designated A/Goose/Jiangsu/YZ527/2011 (H9N2, Gs/JS/YZ527/11), A/Goose/Jiangsu/SQ119/2012 (H9N2, Gs/JS/SQ119/12), and A/Goose/Jiangsu/JD564/2012 (H9N2, Gs/JS/JD564/12), were isolated from domestic geese. Molecular characterization of the three isolates showed that the Gs/JS/YZ527/11 virus is a double-reassortant virus, combining the genes of A/Quail/Hong Kong/G1/97 (H9N2, G1/97) - like and A/Chicken/Shanghai/F/98 (H9N2, F/98) - like; the Gs/JS/SQ119/12 virus is a triple-reassortant virus derived from G1/97 - like, F/98 - like, and A/Duck/Shantou/163/2004 (H9N2, ST/163/04) - like. The sequences of the Gs/JS/JD564/12 virus share high homology with those of the F/98 virus, except for the neuraminidase (NA) gene, and the internal genes of the Gs/JS/YZ527/11 and Gs/JS/SQ119/12 viruses are closely related to those of the H7N9 viruses. An infectivity analysis of the three isolated strains showed that Gs/JS/SQ119/12 and Gs/JS/YZ527/11 replicated well, with seroconversion, in geese and chickens, whereas Gs/JS/JD564/12 was not well infected to either geese or chickens and the F/98 virus only infected chickens, with seroconversion. The emergence of these new reassortant H9N2 influenza viruses indicates that these viruses have infected both chicken and goose with a few types of lesions.
[h=4]KEYWORDS:[/h] H9N2; biological characteristics; chicken; goose; infectivity
[h=1]Identification, sequence analysis, and infectivity of H9N2 avian influenza viruses isolated from geese.[/h] Zhu R[SUP]1,[/SUP][SUP]2[/SUP], Yang X[SUP]1,[/SUP][SUP]2[/SUP], Zhang J[SUP]3[/SUP], Xu D[SUP]1,[/SUP][SUP]2[/SUP], Fan J[SUP]1,[/SUP][SUP]2[/SUP], Shi H[SUP]1,[/SUP][SUP]2[/SUP], Wang S[SUP]4[/SUP], Liu X[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] H9N2 subtype avian influenza virus is a great threat to the Chinese poultry industry, even with annual vaccination. Waterfowl can be asymptomatically infected with the H9N2 virus. In this study, three strains of the H9N2 avian influenza virus, designated A/Goose/Jiangsu/YZ527/2011 (H9N2, Gs/JS/YZ527/11), A/Goose/Jiangsu/SQ119/2012 (H9N2, Gs/JS/SQ119/12), and A/Goose/Jiangsu/JD564/2012 (H9N2, Gs/JS/JD564/12), were isolated from domestic geese. Molecular characterization of the three isolates showed that the Gs/JS/YZ527/11 virus is a double-reassortant virus, combining the genes of A/Quail/Hong Kong/G1/97 (H9N2, G1/97) - like and A/Chicken/Shanghai/F/98 (H9N2, F/98) - like; the Gs/JS/SQ119/12 virus is a triple-reassortant virus derived from G1/97 - like, F/98 - like, and A/Duck/Shantou/163/2004 (H9N2, ST/163/04) - like. The sequences of the Gs/JS/JD564/12 virus share high homology with those of the F/98 virus, except for the neuraminidase (NA) gene, and the internal genes of the Gs/JS/YZ527/11 and Gs/JS/SQ119/12 viruses are closely related to those of the H7N9 viruses. An infectivity analysis of the three isolated strains showed that Gs/JS/SQ119/12 and Gs/JS/YZ527/11 replicated well, with seroconversion, in geese and chickens, whereas Gs/JS/JD564/12 was not well infected to either geese or chickens and the F/98 virus only infected chickens, with seroconversion. The emergence of these new reassortant H9N2 influenza viruses indicates that these viruses have infected both chicken and goose with a few types of lesions.
[h=4]KEYWORDS:[/h] H9N2; biological characteristics; chicken; goose; infectivity