tetano
Editor, Senior Moderator
J Immunol. 2019 Sep 9. pii: ji1900285. doi: 10.4049/jimmunol.1900285. [Epub ahead of print]
[h=1]IgM, IgG, and IgA Influenza-Specific Plasma Cells Express Divergent Transcriptomes.[/h] Price MJ[SUP]1[/SUP], Hicks SL[SUP]1[/SUP], Bradley JE[SUP]2[/SUP], Randall TD[SUP]2[/SUP], Boss JM[SUP]1,[/SUP][SUP]3[/SUP], Scharer CD[SUP]4[/SUP].
[h=3]Author information[/h] 1 Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322. 2 Division of Clinical Immunology and Rheumatology, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294; and. 3 Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322. 4 Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322; cdschar@emory.edu.
[h=3]Abstract[/h] Ab-secreting cells (ASC) or plasma cells are essential components of the humoral immune system. Although Abs of different isotypes have distinct functions, it is not known if the ASC that secrete each isotype are also distinct. ASC downregulate their surface BCR upon differentiation, hindering analyses that couple BCR information to other molecular characteristics. In this study, we developed a methodology using fixation, permeabilization, and intracellular staining coupled with cell sorting and reversal of the cross-links to allow RNA sequencing of isolated cell subsets. Using hemagglutinin and nucleoprotein Ag-specific B cell tetramers and intracellular staining for IgM, IgG, and IgA isotypes, we were able to derive and compare the gene expression programs of ASC subsets that were responding to the same Ags following influenza infection in mice. Intriguingly, whereas a shared ASC signature was identified, each ASC isotype-specific population expressed distinct transcriptional programs controlling cellular homing, metabolism, and potential effector functions. Additionally, we extracted and compared BCR clonotypes and found that each ASC isotype contained a unique, clonally related CDR3 repertoire. In summary, these data reveal specific complexities in the transcriptional programming of Ag-specific ASC populations.
Copyright ? 2019 by The American Association of Immunologists, Inc.
PMID: 31501259 DOI: 10.4049/jimmunol.1900285
[h=1]IgM, IgG, and IgA Influenza-Specific Plasma Cells Express Divergent Transcriptomes.[/h] Price MJ[SUP]1[/SUP], Hicks SL[SUP]1[/SUP], Bradley JE[SUP]2[/SUP], Randall TD[SUP]2[/SUP], Boss JM[SUP]1,[/SUP][SUP]3[/SUP], Scharer CD[SUP]4[/SUP].
[h=3]Author information[/h] 1 Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322. 2 Division of Clinical Immunology and Rheumatology, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294; and. 3 Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322. 4 Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322; cdschar@emory.edu.
[h=3]Abstract[/h] Ab-secreting cells (ASC) or plasma cells are essential components of the humoral immune system. Although Abs of different isotypes have distinct functions, it is not known if the ASC that secrete each isotype are also distinct. ASC downregulate their surface BCR upon differentiation, hindering analyses that couple BCR information to other molecular characteristics. In this study, we developed a methodology using fixation, permeabilization, and intracellular staining coupled with cell sorting and reversal of the cross-links to allow RNA sequencing of isolated cell subsets. Using hemagglutinin and nucleoprotein Ag-specific B cell tetramers and intracellular staining for IgM, IgG, and IgA isotypes, we were able to derive and compare the gene expression programs of ASC subsets that were responding to the same Ags following influenza infection in mice. Intriguingly, whereas a shared ASC signature was identified, each ASC isotype-specific population expressed distinct transcriptional programs controlling cellular homing, metabolism, and potential effector functions. Additionally, we extracted and compared BCR clonotypes and found that each ASC isotype contained a unique, clonally related CDR3 repertoire. In summary, these data reveal specific complexities in the transcriptional programming of Ag-specific ASC populations.
Copyright ? 2019 by The American Association of Immunologists, Inc.
PMID: 31501259 DOI: 10.4049/jimmunol.1900285