tetano
Editor, Senior Moderator
Sci Rep. 2014 May 2;4:4877. doi: 10.1038/srep04877.
Imaging of influenza virus sialidase activity in living cells.
Kurebayashi Y1, Takahashi T1, Otsubo T2, Ikeda K2, Takahashi S3, Takano M3, Agarikuchi T3, Sato T3, Matsuda Y3, Minami A3, Kanazawa H4, Uchida Y5, Saito T5, Kawaoka Y6, Yamada T7, Kawamori F7, Thomson R8, von Itzstein M8, Suzuki T3.
Author information
Abstract
Influenza virus is rich in variation and mutations. It would be very convenient for virus detection and isolation to histochemically detect viral infection regardless of variation and mutations. Here, we established a histochemical imaging assay for influenza virus sialidase activity in living cells by using a new fluorescent sialidase substrate, 2-(benzothiazol-2-yl)-4-bromophenyl 5-acetamido-3,5-dideoxy-α-D-glycero-D-galacto-2-nonulopyranosidonic acid (BTP3-Neu5Ac). The BTP3-Neu5Ac assay histochemically visualized influenza virus-infected cells regardless of viral hosts and subtypes. Influenza virus neuraminidase-expressed cells, viral focus formation, and virus-infected locations in mice lung tissues were easily, rapidly, and sensitively detected by the BTP3-Neu5Ac assay. Histochemical visualization with the BTP3-Neu5Ac assay is extremely useful for detection of influenza viruses without the need for fixation or a specific antibody. This novel assay should greatly improve the efficiency of detection, titration, and isolation of influenza viruses and might contribute to research on viral sialidase.
PMID:
24786761
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24786761
Imaging of influenza virus sialidase activity in living cells.
Kurebayashi Y1, Takahashi T1, Otsubo T2, Ikeda K2, Takahashi S3, Takano M3, Agarikuchi T3, Sato T3, Matsuda Y3, Minami A3, Kanazawa H4, Uchida Y5, Saito T5, Kawaoka Y6, Yamada T7, Kawamori F7, Thomson R8, von Itzstein M8, Suzuki T3.
Author information
Abstract
Influenza virus is rich in variation and mutations. It would be very convenient for virus detection and isolation to histochemically detect viral infection regardless of variation and mutations. Here, we established a histochemical imaging assay for influenza virus sialidase activity in living cells by using a new fluorescent sialidase substrate, 2-(benzothiazol-2-yl)-4-bromophenyl 5-acetamido-3,5-dideoxy-α-D-glycero-D-galacto-2-nonulopyranosidonic acid (BTP3-Neu5Ac). The BTP3-Neu5Ac assay histochemically visualized influenza virus-infected cells regardless of viral hosts and subtypes. Influenza virus neuraminidase-expressed cells, viral focus formation, and virus-infected locations in mice lung tissues were easily, rapidly, and sensitively detected by the BTP3-Neu5Ac assay. Histochemical visualization with the BTP3-Neu5Ac assay is extremely useful for detection of influenza viruses without the need for fixation or a specific antibody. This novel assay should greatly improve the efficiency of detection, titration, and isolation of influenza viruses and might contribute to research on viral sialidase.
PMID:
24786761
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24786761