tetano
Editor, Senior Moderator
Acta Microbiol Immunol Hung. 2017 Jun 19:1-18. doi: 10.1556/030.64.2017.011. [Epub ahead of print]
[h=1]Immune responses to killed reassorted influenza virus supplemented with natural adjuvants.[/h] Razin MAF[SUP]1[/SUP], Osman A[SUP]2[/SUP], Ali MA[SUP]3[/SUP], Bahgat MM[SUP]4[/SUP], Maghraby AS[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In this study, we investigated the immunomodulatory effects of a supplemented killed influenza virus (V) by Echinacea purpurea (E) and Nigella sativa (N) extracts and effect of changing the route of immunization from intramuscular (IM) to intraperitoneal (IP). At the 2[SUP]nd[/SUP]-, 3[SUP]rd[/SUP]- and 4[SUP]th[/SUP]-week post-IM immunizations (WPIMI), the supplemented V with N (VN) induced the most significant IgM response unlike N alone. At the 2[SUP]nd[/SUP] WPIMI, V or VN induced the highest significant IgG levels. At the 2[SUP]nd[/SUP]-week post-IP immunization (WPIPI), E and VN induced the most significant IgG levels. Both at the 3[SUP]rd[/SUP] and 4[SUP]th[/SUP] WPIMI or WPIPI, various treatments induced significant increases in IgG. At the 4[SUP]th[/SUP] WPIMI, E, V, and V with E (VE) induced significant increases in the CD4+ thymocytes while all IP treatments caused significant increase in their counts. V and VN induced the most significant IM induction of CD8+ thymocytes while their best IP stimulation was induced by N, VE, and VN. At the 4[SUP]th[/SUP] WPIMI, various treatments caused significant increases in the mesenteric lymph node (MLN) CD4+, CD8+ counts. WPIPI with V or VE caused significant increases in both the CD4+- and CD8+ MLN cells, whereas VN significantly induced CD8+ MLN cells only. WPIPI with various treatments caused significant increases in the B-cell counts and the peak was obtained by VN.
[h=4]KEYWORDS:[/h] Echinacea purpurea; Nigella sativa; avian influenza; immunoglobulins; lymphocytes; reassortment
PMID: 28627238 DOI: 10.1556/030.64.2017.011
[h=1]Immune responses to killed reassorted influenza virus supplemented with natural adjuvants.[/h] Razin MAF[SUP]1[/SUP], Osman A[SUP]2[/SUP], Ali MA[SUP]3[/SUP], Bahgat MM[SUP]4[/SUP], Maghraby AS[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In this study, we investigated the immunomodulatory effects of a supplemented killed influenza virus (V) by Echinacea purpurea (E) and Nigella sativa (N) extracts and effect of changing the route of immunization from intramuscular (IM) to intraperitoneal (IP). At the 2[SUP]nd[/SUP]-, 3[SUP]rd[/SUP]- and 4[SUP]th[/SUP]-week post-IM immunizations (WPIMI), the supplemented V with N (VN) induced the most significant IgM response unlike N alone. At the 2[SUP]nd[/SUP] WPIMI, V or VN induced the highest significant IgG levels. At the 2[SUP]nd[/SUP]-week post-IP immunization (WPIPI), E and VN induced the most significant IgG levels. Both at the 3[SUP]rd[/SUP] and 4[SUP]th[/SUP] WPIMI or WPIPI, various treatments induced significant increases in IgG. At the 4[SUP]th[/SUP] WPIMI, E, V, and V with E (VE) induced significant increases in the CD4+ thymocytes while all IP treatments caused significant increase in their counts. V and VN induced the most significant IM induction of CD8+ thymocytes while their best IP stimulation was induced by N, VE, and VN. At the 4[SUP]th[/SUP] WPIMI, various treatments caused significant increases in the mesenteric lymph node (MLN) CD4+, CD8+ counts. WPIPI with V or VE caused significant increases in both the CD4+- and CD8+ MLN cells, whereas VN significantly induced CD8+ MLN cells only. WPIPI with various treatments caused significant increases in the B-cell counts and the peak was obtained by VN.
[h=4]KEYWORDS:[/h] Echinacea purpurea; Nigella sativa; avian influenza; immunoglobulins; lymphocytes; reassortment
PMID: 28627238 DOI: 10.1556/030.64.2017.011