tetano
Editor, Senior Moderator
Immunity
. 2022 Jun 8;S1074-7613(22)00241-2.
doi: 10.1016/j.immuni.2022.06.003. Online ahead of print.
SARS-CoV-2-specific T cell memory with common TCRαβ motifs is established in unvaccinated children who seroconvert after infection
Louise C Rowntree[SUP] 1 [/SUP], Thi H O Nguyen[SUP] 1 [/SUP], Lukasz Kedzierski[SUP] 2 [/SUP], Melanie R Neeland[SUP] 3 [/SUP], Jan Petersen[SUP] 4 [/SUP], Jeremy Chase Crawford[SUP] 5 [/SUP], Lilith F Allen[SUP] 1 [/SUP], E Bridie Clemens[SUP] 1 [/SUP], Brendon Chua[SUP] 1 [/SUP], Hayley A McQuilten[SUP] 1 [/SUP], Anastasia A Minervina[SUP] 5 [/SUP], Mikhail V Pogorelyy[SUP] 5 [/SUP], Priyanka Chaurasia[SUP] 4 [/SUP], Hyon-Xhi Tan[SUP] 1 [/SUP], Adam K Wheatley[SUP] 6 [/SUP], Xiaoxiao Jia[SUP] 1 [/SUP], Fatima Amanat[SUP] 7 [/SUP], Florian Krammer[SUP] 8 [/SUP], E Kaitlynn Allen[SUP] 5 [/SUP], Sabrina Sonda[SUP] 9 [/SUP], Katie L Flanagan[SUP] 10 [/SUP], Jaycee Jumarang[SUP] 11 [/SUP], Pia S Pannaraj[SUP] 12 [/SUP], Paul V Licciardi[SUP] 3 [/SUP], Stephen J Kent[SUP] 13 [/SUP], Katherine A Bond[SUP] 14 [/SUP], Deborah A Williamson[SUP] 15 [/SUP], Jamie Rossjohn[SUP] 16 [/SUP], Paul G Thomas[SUP] 5 [/SUP], Shidan Tosif[SUP] 17 [/SUP], Nigel W Crawford[SUP] 18 [/SUP], Carolien E van de Sandt[SUP] 1 [/SUP], Katherine Kedzierska[SUP] 19 [/SUP]
Affiliations
Abstract
As the establishment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T cell memory in children remains largely unexplored, we recruited convalescent COVID-19 children and adults to define their circulating memory SARS-CoV-2-specific CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T cells prior to vaccination. We analyzed epitope-specific T cells directly ex vivo using seven HLA class I and class II tetramers presenting SARS-CoV-2 epitopes, together with Spike-specific B cells. Unvaccinated children who seroconverted had comparable Spike-specific but lower ORF1a- and N-specific memory T cell responses compared with adults. This agreed with our TCR sequencing data showing reduced clonal expansion in children. A strong stem cell memory phenotype and common T cell receptor motifs were detected within tetramer-specific T cells in seroconverted children. Conversely, children who did not seroconvert had tetramer-specific T cells of predominantly naive phenotypes and diverse TCRαβ repertoires. Our study demonstrates the generation of SARS-CoV-2-specific T cell memory with common TCRαβ motifs in unvaccinated seroconverted children after their first virus encounter.
Keywords: B cells; CD4(+) T cells; CD8(+) T cells; COVID-19; SARS-CoV-2; children; memory T cells; tetramer-specific.
. 2022 Jun 8;S1074-7613(22)00241-2.
doi: 10.1016/j.immuni.2022.06.003. Online ahead of print.
SARS-CoV-2-specific T cell memory with common TCRαβ motifs is established in unvaccinated children who seroconvert after infection
Louise C Rowntree[SUP] 1 [/SUP], Thi H O Nguyen[SUP] 1 [/SUP], Lukasz Kedzierski[SUP] 2 [/SUP], Melanie R Neeland[SUP] 3 [/SUP], Jan Petersen[SUP] 4 [/SUP], Jeremy Chase Crawford[SUP] 5 [/SUP], Lilith F Allen[SUP] 1 [/SUP], E Bridie Clemens[SUP] 1 [/SUP], Brendon Chua[SUP] 1 [/SUP], Hayley A McQuilten[SUP] 1 [/SUP], Anastasia A Minervina[SUP] 5 [/SUP], Mikhail V Pogorelyy[SUP] 5 [/SUP], Priyanka Chaurasia[SUP] 4 [/SUP], Hyon-Xhi Tan[SUP] 1 [/SUP], Adam K Wheatley[SUP] 6 [/SUP], Xiaoxiao Jia[SUP] 1 [/SUP], Fatima Amanat[SUP] 7 [/SUP], Florian Krammer[SUP] 8 [/SUP], E Kaitlynn Allen[SUP] 5 [/SUP], Sabrina Sonda[SUP] 9 [/SUP], Katie L Flanagan[SUP] 10 [/SUP], Jaycee Jumarang[SUP] 11 [/SUP], Pia S Pannaraj[SUP] 12 [/SUP], Paul V Licciardi[SUP] 3 [/SUP], Stephen J Kent[SUP] 13 [/SUP], Katherine A Bond[SUP] 14 [/SUP], Deborah A Williamson[SUP] 15 [/SUP], Jamie Rossjohn[SUP] 16 [/SUP], Paul G Thomas[SUP] 5 [/SUP], Shidan Tosif[SUP] 17 [/SUP], Nigel W Crawford[SUP] 18 [/SUP], Carolien E van de Sandt[SUP] 1 [/SUP], Katherine Kedzierska[SUP] 19 [/SUP]
Affiliations
- PMID: 35750048
- PMCID: PMC9174177
- DOI: 10.1016/j.immuni.2022.06.003
Abstract
As the establishment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T cell memory in children remains largely unexplored, we recruited convalescent COVID-19 children and adults to define their circulating memory SARS-CoV-2-specific CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T cells prior to vaccination. We analyzed epitope-specific T cells directly ex vivo using seven HLA class I and class II tetramers presenting SARS-CoV-2 epitopes, together with Spike-specific B cells. Unvaccinated children who seroconverted had comparable Spike-specific but lower ORF1a- and N-specific memory T cell responses compared with adults. This agreed with our TCR sequencing data showing reduced clonal expansion in children. A strong stem cell memory phenotype and common T cell receptor motifs were detected within tetramer-specific T cells in seroconverted children. Conversely, children who did not seroconvert had tetramer-specific T cells of predominantly naive phenotypes and diverse TCRαβ repertoires. Our study demonstrates the generation of SARS-CoV-2-specific T cell memory with common TCRαβ motifs in unvaccinated seroconverted children after their first virus encounter.
Keywords: B cells; CD4(+) T cells; CD8(+) T cells; COVID-19; SARS-CoV-2; children; memory T cells; tetramer-specific.