tetano
Editor, Senior Moderator
Zhongguo Zhong Yao Za Zhi. 2018 Mar;43(5):1028-1033. doi: 10.19540/j.cnki.cjcmm.20180104.018.
[h=1][Immunologic mechanisms of Yinhua Pinggan granule and San-ao decoction against influenza virus in vivo].[/h] [Article in Chinese]
Du HX[SUP]1[/SUP], Zhou HF[SUP]1[/SUP], He Y[SUP]1[/SUP], Yang JH[SUP]1[/SUP], Lu YY[SUP]2[/SUP], Fan YC[SUP]1[/SUP], Wan HT[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] This paper aimed to investigate the effect of Yinhua Pinggan granule and San-ao decoction on the immunologic mechanisms of influenza viral pneumonia mice in vivo, in order to study the activity of the combined administration of different formulas on influenza A/H1N1 virus. The model of pneumonia was established in mice through nasal dropping influenza virus, and then divided randomly into five groups: normal control group, influenza virus model group, oseltamivir control group, Yinhua Pinggan granule group, and San-ao decoction group. The animals were put to death at the 5th day after gavage administration with the corresponding drugs. The contents in mice serum of TNF-α, IL-6 and IFN-γ were respectively measured by ELISA. The mRNA expressions of TLR3/7, MyD88, JNK, p38MAPK and NF-κB p65 in lung tissues were respectively detected by RT-PCR. The protein expressions of JNK, p38MAPK and NF-κB p65 in lung tissues were determined by immunohistochemical analysis, respectively. According to the results, Yinhua Pinggan granule and San-ao decoction could significantly decrease the levels of TNF-α and IL-6, increase the level of IFN-γ in mice serum of lung tissues, significantly reduce the gene expressions of TLR3/7, MyD88, JNK, p38MAPK and NF-κB p65 in influenza virus-infected mice lung tissues, and significantly reduce the protein expressions of JNK, p38MAPK and NF-κB p65 in lung tissues. Furthermore, the regulatory effect of Yinhua Pinggan granule was superior to that of San-ao decoction. In conclusion, Yinhua Pingan granule and San-ao decoction have the therapeutic effect on pneumonia mice infected by H1N1 virus in vivo. The anti-influenza mechanisms of Yinhua Pinggan granule and San-ao decoction may be the results of interactions by regulating the immunologic function of influenza virus-infected mice and TLR3/7 signaling pathway with multiple links of the gene and protein expressions. Moreover, the combined administration of warm-natured and cold-natured Yinhua Pinggan granule with the effects of detoxification and exhalation has a better effect than the single administration of warm-natured San-ao decoction.
[h=4]KEYWORDS:[/h] San-ao decotion ; TLRs signaling pathway ; Yinhua Pinggan granule ; compatibility of formula ; influenza A/H1N1 virus ; mice with influenza viral pneumonia
PMID: 29676104
[h=1][Immunologic mechanisms of Yinhua Pinggan granule and San-ao decoction against influenza virus in vivo].[/h] [Article in Chinese]
Du HX[SUP]1[/SUP], Zhou HF[SUP]1[/SUP], He Y[SUP]1[/SUP], Yang JH[SUP]1[/SUP], Lu YY[SUP]2[/SUP], Fan YC[SUP]1[/SUP], Wan HT[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] This paper aimed to investigate the effect of Yinhua Pinggan granule and San-ao decoction on the immunologic mechanisms of influenza viral pneumonia mice in vivo, in order to study the activity of the combined administration of different formulas on influenza A/H1N1 virus. The model of pneumonia was established in mice through nasal dropping influenza virus, and then divided randomly into five groups: normal control group, influenza virus model group, oseltamivir control group, Yinhua Pinggan granule group, and San-ao decoction group. The animals were put to death at the 5th day after gavage administration with the corresponding drugs. The contents in mice serum of TNF-α, IL-6 and IFN-γ were respectively measured by ELISA. The mRNA expressions of TLR3/7, MyD88, JNK, p38MAPK and NF-κB p65 in lung tissues were respectively detected by RT-PCR. The protein expressions of JNK, p38MAPK and NF-κB p65 in lung tissues were determined by immunohistochemical analysis, respectively. According to the results, Yinhua Pinggan granule and San-ao decoction could significantly decrease the levels of TNF-α and IL-6, increase the level of IFN-γ in mice serum of lung tissues, significantly reduce the gene expressions of TLR3/7, MyD88, JNK, p38MAPK and NF-κB p65 in influenza virus-infected mice lung tissues, and significantly reduce the protein expressions of JNK, p38MAPK and NF-κB p65 in lung tissues. Furthermore, the regulatory effect of Yinhua Pinggan granule was superior to that of San-ao decoction. In conclusion, Yinhua Pingan granule and San-ao decoction have the therapeutic effect on pneumonia mice infected by H1N1 virus in vivo. The anti-influenza mechanisms of Yinhua Pinggan granule and San-ao decoction may be the results of interactions by regulating the immunologic function of influenza virus-infected mice and TLR3/7 signaling pathway with multiple links of the gene and protein expressions. Moreover, the combined administration of warm-natured and cold-natured Yinhua Pinggan granule with the effects of detoxification and exhalation has a better effect than the single administration of warm-natured San-ao decoction.
[h=4]KEYWORDS:[/h] San-ao decotion ; TLRs signaling pathway ; Yinhua Pinggan granule ; compatibility of formula ; influenza A/H1N1 virus ; mice with influenza viral pneumonia
PMID: 29676104