tetano
Editor, Senior Moderator
Clin Nutr. 2017 Jan 28. pii: S0261-5614(17)30035-3. doi: 10.1016/j.clnu.2017.01.011. [Epub ahead of print]
[h=1]Impact of ageing and a synbiotic on the immune response to seasonal influenza vaccination; a randomised controlled trial.[/h] Enani S[SUP]1[/SUP], Przemska-Kosicka A[SUP]1[/SUP], Childs CE[SUP]1[/SUP], Maidens C[SUP]1[/SUP], Dong H[SUP]1[/SUP], Conterno L[SUP]2[/SUP], Tuohy K[SUP]2[/SUP], Todd S[SUP]3[/SUP], Gosney M[SUP]1[/SUP], Yaqoob P[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND & AIMS:[/h] Ageing increases risk of respiratory infections and impairs the response to influenza vaccination. Pre- and pro-biotics offer an opportunity to modulate anti-viral defenses and the response to vaccination via alteration of the gut microbiota. This study investigated the effect of a novel probiotic, Bifidobacterium longum bv. infantis CCUG 52486, combined with a prebiotic, gluco-oligosaccharide, on the B and T cell response to seasonal influenza vaccination in young and older subjects .
[h=4]METHODS:[/h] In a double-blind, randomized controlled trial, 58 young (18-35 y) and 54 older (60-85 y) subjects were supplemented with the synbiotic for 8 weeks. At 4 weeks they were administered with a seasonal influenza vaccine. B and T cell phenotype and responsiveness to in vitro re-stimulation with the vaccine were assessed at baseline, 4, 6 and 8 weeks.
[h=4]RESULTS:[/h] B and T cell profiles differed markedly between young and older subjects. Vaccination increased numbers of memory, IgA[SUP]+[/SUP] memory, IgG[SUP]+[/SUP] memory and total IgG[SUP]+[/SUP] B cells in young subjects, but failed to do so in older subjects and did not significantly alter T cell subsets. Seroconversion to the H1N1 subunit in the older subjects was associated with higher post-vaccination numbers of plasma B cells, but seroconversion was less consistently associated with T cell phenotype. B and T cell subsets from both young and older subjects demonstrated a strong antigen-specific recall challenge, and although not influenced by age, responsiveness to the recall challenge was associated with seroconversion. In older subjects, CMV seropositivity was associated with a significantly lower recall response to the vaccine, but the synbiotic did not affect the responsiveness of B or T cells to re-stimulation with influenza vaccine.
[h=4]CONCLUSIONS:[/h] Antigen-specific B and T cell activation following an in vitro recall challenge with the influenza vaccine was influenced by CMV seropositivity, but not by a synbiotic. Registered under ClinicalTrials.gov Identifier no. NCT01066377.
Copyright ? 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.
[h=4]KEYWORDS:[/h] Ageing; Influenza; Lymphocyte; Probiotic; Vaccination
PMID: 28215759 DOI: 10.1016/j.clnu.2017.01.011
[PubMed - as supplied by publisher]
[h=1]Impact of ageing and a synbiotic on the immune response to seasonal influenza vaccination; a randomised controlled trial.[/h] Enani S[SUP]1[/SUP], Przemska-Kosicka A[SUP]1[/SUP], Childs CE[SUP]1[/SUP], Maidens C[SUP]1[/SUP], Dong H[SUP]1[/SUP], Conterno L[SUP]2[/SUP], Tuohy K[SUP]2[/SUP], Todd S[SUP]3[/SUP], Gosney M[SUP]1[/SUP], Yaqoob P[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND & AIMS:[/h] Ageing increases risk of respiratory infections and impairs the response to influenza vaccination. Pre- and pro-biotics offer an opportunity to modulate anti-viral defenses and the response to vaccination via alteration of the gut microbiota. This study investigated the effect of a novel probiotic, Bifidobacterium longum bv. infantis CCUG 52486, combined with a prebiotic, gluco-oligosaccharide, on the B and T cell response to seasonal influenza vaccination in young and older subjects .
[h=4]METHODS:[/h] In a double-blind, randomized controlled trial, 58 young (18-35 y) and 54 older (60-85 y) subjects were supplemented with the synbiotic for 8 weeks. At 4 weeks they were administered with a seasonal influenza vaccine. B and T cell phenotype and responsiveness to in vitro re-stimulation with the vaccine were assessed at baseline, 4, 6 and 8 weeks.
[h=4]RESULTS:[/h] B and T cell profiles differed markedly between young and older subjects. Vaccination increased numbers of memory, IgA[SUP]+[/SUP] memory, IgG[SUP]+[/SUP] memory and total IgG[SUP]+[/SUP] B cells in young subjects, but failed to do so in older subjects and did not significantly alter T cell subsets. Seroconversion to the H1N1 subunit in the older subjects was associated with higher post-vaccination numbers of plasma B cells, but seroconversion was less consistently associated with T cell phenotype. B and T cell subsets from both young and older subjects demonstrated a strong antigen-specific recall challenge, and although not influenced by age, responsiveness to the recall challenge was associated with seroconversion. In older subjects, CMV seropositivity was associated with a significantly lower recall response to the vaccine, but the synbiotic did not affect the responsiveness of B or T cells to re-stimulation with influenza vaccine.
[h=4]CONCLUSIONS:[/h] Antigen-specific B and T cell activation following an in vitro recall challenge with the influenza vaccine was influenced by CMV seropositivity, but not by a synbiotic. Registered under ClinicalTrials.gov Identifier no. NCT01066377.
Copyright ? 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.
[h=4]KEYWORDS:[/h] Ageing; Influenza; Lymphocyte; Probiotic; Vaccination
PMID: 28215759 DOI: 10.1016/j.clnu.2017.01.011
[PubMed - as supplied by publisher]