tetano
Editor, Senior Moderator
Int J Biol Macromol. 2016 Jun 20. pii: S0141-8130(16)30595-5. doi: 10.1016/j.ijbiomac.2016.06.057. [Epub ahead of print]
[h=1]Improvement of immune responses to influenza vaccine (H5N1) by sulfated yeast beta-glucan.[/h] Wang M[SUP]1[/SUP], Zhang L[SUP]2[/SUP], Yang R[SUP]2[/SUP], Fei C[SUP]2[/SUP], Wang X[SUP]2[/SUP], Zhang K[SUP]2[/SUP], Wang C[SUP]2[/SUP], Zheng W[SUP]2[/SUP], Xue F[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In this study, the adjuvant activity of sulfated glucan from saccharomyces cerevisiae ([SUB]S[/SUB]GSC) was investigated. sGSC had molecular weight of 12.9kDa and degrees of sulfation of 0.16.The effects of [SUB]S[/SUB]GSC on the splenic lymphocyte cells of mice were measured in vitro. The results showed that [SUB]S[/SUB]GSC could significantly promote lymphocyte proliferation singly or synergistically with Con A and LPS, and stimulate the cells to secrete IL-2 and INF-γ. The adjuvant activity of [SUB]S[/SUB]GSC was researched in BALB/c mice inoculated with inactivated H5N1vaccine in vivo. The results showed that [SUB]S[/SUB]GSC could significantly enhance lymphocyte proliferation, effectively increase the percentage of CD4[SUP]+[/SUP] T cells, decrease the percentage of CD8[SUP]+[/SUP] T cells, and elevate the CD4[SUP]+[/SUP]/CD8[SUP]+[/SUP] ratio; enhance the HI antibody titre, and promote the production of IL-2, INF-γ, IL-4 and IL-6 at medium level. These results indicated that sulfated glucan showed an excellent adjuvant effect on H5N1vaccine in a mouse model. Therefore, [SUB]S[/SUB]GSC could be used as an effective immune adjuvant for an inactivated H5N1vaccine.
Copyright ? 2016. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Adjuvant activity; H5N1vaccine; Sulfated glucan from saccharomyces cerevisiae
PMID: 27339320 DOI: 10.1016/j.ijbiomac.2016.06.057
[PubMed - as supplied by publisher]
[h=1]Improvement of immune responses to influenza vaccine (H5N1) by sulfated yeast beta-glucan.[/h] Wang M[SUP]1[/SUP], Zhang L[SUP]2[/SUP], Yang R[SUP]2[/SUP], Fei C[SUP]2[/SUP], Wang X[SUP]2[/SUP], Zhang K[SUP]2[/SUP], Wang C[SUP]2[/SUP], Zheng W[SUP]2[/SUP], Xue F[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In this study, the adjuvant activity of sulfated glucan from saccharomyces cerevisiae ([SUB]S[/SUB]GSC) was investigated. sGSC had molecular weight of 12.9kDa and degrees of sulfation of 0.16.The effects of [SUB]S[/SUB]GSC on the splenic lymphocyte cells of mice were measured in vitro. The results showed that [SUB]S[/SUB]GSC could significantly promote lymphocyte proliferation singly or synergistically with Con A and LPS, and stimulate the cells to secrete IL-2 and INF-γ. The adjuvant activity of [SUB]S[/SUB]GSC was researched in BALB/c mice inoculated with inactivated H5N1vaccine in vivo. The results showed that [SUB]S[/SUB]GSC could significantly enhance lymphocyte proliferation, effectively increase the percentage of CD4[SUP]+[/SUP] T cells, decrease the percentage of CD8[SUP]+[/SUP] T cells, and elevate the CD4[SUP]+[/SUP]/CD8[SUP]+[/SUP] ratio; enhance the HI antibody titre, and promote the production of IL-2, INF-γ, IL-4 and IL-6 at medium level. These results indicated that sulfated glucan showed an excellent adjuvant effect on H5N1vaccine in a mouse model. Therefore, [SUB]S[/SUB]GSC could be used as an effective immune adjuvant for an inactivated H5N1vaccine.
Copyright ? 2016. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Adjuvant activity; H5N1vaccine; Sulfated glucan from saccharomyces cerevisiae
PMID: 27339320 DOI: 10.1016/j.ijbiomac.2016.06.057
[PubMed - as supplied by publisher]