tetano
Editor, Senior Moderator
J Gen Virol. 2012 Dec 19. [Epub ahead of print]
IN VITRO AND IN VIVO REPLICATION OF INFLUENZA A H1N1 WSN33 VIRUSES WITH DIFFERENT M1 PROTEINS.
Ran Z, Chen Y, Shen H, Xiang X, Liu Q, Bawa B, Qi W, Zhu L, Young A, Richt JA, Ma W, Li F.
Source
South Dakota State University, SD, USA;
Abstract
The M1 protein is a major structural protein which has multiple functions in various steps within the lifecycle of the influenza A virus. However, little is currently known about the role of M1 in influenza A virus replication in vivo and the associated pathogenesis. In this study, six isogenic H1N1 WSN33 viruses, constructed to express unique M1 proteins derived from various strains, subtypes, or WSN33 itself, were tested to determine in vitro and in vivo functional exchangeability of M1 proteins in the replication and pathogenesis of the WSN33 virus. Despite five chimeric M1 viruses replicating to levels similar to those of the parental WSN33 virus in cell cultures, all M1 chimeras exhibited improved replication and enhanced virulence in mice when compared to the WSN33 virus. Interestingly, M1 proteins derived from swine viruses caused more severe clinical diseases than those from human or quail. These data indicate that the M1 protein is an important determinant of viral replication and pathogenic properties in mice, although the functions of M1 observed in vivo are not adequately reflected in simple infections of cultured cells. Chimeric M1 viruses variable in clinical manifestations described here will aid future understanding of the role of M1 in influenza A virus pathogenesis.
PMID:
23255622
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23255622
IN VITRO AND IN VIVO REPLICATION OF INFLUENZA A H1N1 WSN33 VIRUSES WITH DIFFERENT M1 PROTEINS.
Ran Z, Chen Y, Shen H, Xiang X, Liu Q, Bawa B, Qi W, Zhu L, Young A, Richt JA, Ma W, Li F.
Source
South Dakota State University, SD, USA;
Abstract
The M1 protein is a major structural protein which has multiple functions in various steps within the lifecycle of the influenza A virus. However, little is currently known about the role of M1 in influenza A virus replication in vivo and the associated pathogenesis. In this study, six isogenic H1N1 WSN33 viruses, constructed to express unique M1 proteins derived from various strains, subtypes, or WSN33 itself, were tested to determine in vitro and in vivo functional exchangeability of M1 proteins in the replication and pathogenesis of the WSN33 virus. Despite five chimeric M1 viruses replicating to levels similar to those of the parental WSN33 virus in cell cultures, all M1 chimeras exhibited improved replication and enhanced virulence in mice when compared to the WSN33 virus. Interestingly, M1 proteins derived from swine viruses caused more severe clinical diseases than those from human or quail. These data indicate that the M1 protein is an important determinant of viral replication and pathogenic properties in mice, although the functions of M1 observed in vivo are not adequately reflected in simple infections of cultured cells. Chimeric M1 viruses variable in clinical manifestations described here will aid future understanding of the role of M1 in influenza A virus pathogenesis.
PMID:
23255622
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23255622