tetano
Editor, Senior Moderator
BMC Res Notes. 2018 Jul 3;11(1):428. doi: 10.1186/s13104-018-3537-9.
[h=1]Induction of immune response in chickens primed in ovo with an inactivated H9N2 avian influenza virus vaccine.[/h] Astill J[SUP]1[/SUP], Alkie T[SUP]1,[/SUP][SUP]2[/SUP], Yitbarek A[SUP]1[/SUP], Taha-Abdelaziz K[SUP]1,[/SUP][SUP]3[/SUP], Bavananthasivam J[SUP]1[/SUP], Nagy ?[SUP]1[/SUP], Petrik JJ[SUP]4[/SUP], Sharif S[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVE:[/h] Infection of chickens with low pathogenic avian influenza virus, such as H9N2 virus, culminates in decreased egg production and increased mortality and morbidity if co-infection with other respiratory pathogens occurs. We have previously observed the induction of antibody- and cell-mediated immune responses after intramuscular administration of an H9N2 beta-propiolactone inactivated virus vaccine to chickens. Given the fact that in ovo vaccination represents a practical option for vaccination against H9N2 AIV in chickens, in the current study, we set out to characterize immune responses in chickens against a beta-propiolactone inactivated H9N2 virus vaccine after primary vaccination in ovo on embryonic day 18, and secondary intramuscular vaccination on day 14 post-hatch. We also included the Toll-like receptor 21 ligand, CpG ODN 2007, and an oil emulsion adjuvant, AddaVax?, as adjuvants for the vaccines.
[h=4]RESULTS:[/h] Antibody-mediated immune responses were observed after administering the secondary intramuscular vaccine. Cell-mediated immune responses were observed in chickens that received the beta-propiolactone inactivated H9N2 virus combined with AddaVax?. Our results demonstrate that adaptive immune responses can be induced in chickens after a primary in ovo vaccination and secondary intramuscular vaccination.
[h=4]KEYWORDS:[/h] Antibody; Beta-propiolactone; Cell-mediated; CpG ODN; H9N2 avian influenza virus; In ovo; Intramuscular; Toll-like receptor 21; Vaccine
PMID: 29970157 DOI: 10.1186/s13104-018-3537-9
Free full text
[h=1]Induction of immune response in chickens primed in ovo with an inactivated H9N2 avian influenza virus vaccine.[/h] Astill J[SUP]1[/SUP], Alkie T[SUP]1,[/SUP][SUP]2[/SUP], Yitbarek A[SUP]1[/SUP], Taha-Abdelaziz K[SUP]1,[/SUP][SUP]3[/SUP], Bavananthasivam J[SUP]1[/SUP], Nagy ?[SUP]1[/SUP], Petrik JJ[SUP]4[/SUP], Sharif S[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVE:[/h] Infection of chickens with low pathogenic avian influenza virus, such as H9N2 virus, culminates in decreased egg production and increased mortality and morbidity if co-infection with other respiratory pathogens occurs. We have previously observed the induction of antibody- and cell-mediated immune responses after intramuscular administration of an H9N2 beta-propiolactone inactivated virus vaccine to chickens. Given the fact that in ovo vaccination represents a practical option for vaccination against H9N2 AIV in chickens, in the current study, we set out to characterize immune responses in chickens against a beta-propiolactone inactivated H9N2 virus vaccine after primary vaccination in ovo on embryonic day 18, and secondary intramuscular vaccination on day 14 post-hatch. We also included the Toll-like receptor 21 ligand, CpG ODN 2007, and an oil emulsion adjuvant, AddaVax?, as adjuvants for the vaccines.
[h=4]RESULTS:[/h] Antibody-mediated immune responses were observed after administering the secondary intramuscular vaccine. Cell-mediated immune responses were observed in chickens that received the beta-propiolactone inactivated H9N2 virus combined with AddaVax?. Our results demonstrate that adaptive immune responses can be induced in chickens after a primary in ovo vaccination and secondary intramuscular vaccination.
[h=4]KEYWORDS:[/h] Antibody; Beta-propiolactone; Cell-mediated; CpG ODN; H9N2 avian influenza virus; In ovo; Intramuscular; Toll-like receptor 21; Vaccine
PMID: 29970157 DOI: 10.1186/s13104-018-3537-9
Free full text