tetano
Editor, Senior Moderator
Infect Disord Drug Targets
. 2022 Aug 29.
doi: 10.2174/1871526522666220829153042. Online ahead of print.
Human ACE-2, MCP1 and micro-RNA 146 as Novel Markers for COVID- 19 Affection and Severity
Amal Ahmed Mohamed[SUP] 1 [/SUP], Sherief Abd-Elsalam[SUP] 2 [/SUP], Ahmed Abdelghani[SUP] 3 [/SUP], Mohamed Badr Hassan[SUP] 3 [/SUP], Doaa Ghaith[SUP] 4 [/SUP], Omnia Ezzat[SUP] 5 [/SUP], Dalia Ali El-Damasy[SUP] 6 [/SUP], Norhan Nagdi Madbouli[SUP] 7 [/SUP], Mohmoud Hamada[SUP] 8 [/SUP], Mohamed Abdel Khalik Hepatology Gastroenterology And Infectious Diseases[SUP] 9 [/SUP], Shaimaa M Al-Tabbakh[SUP] 10 [/SUP], Kareman Ahmed Ebrahim Eshra[SUP] 11 [/SUP], Nivin Baiomy[SUP] 12 [/SUP]
Affiliations
Abstract
Background & aims: Coronavirus disease - 2019 (COVID-19) is a major pandemic that causes high morbidity and mortality rates.
Aim of this study: to detect the relations between many risk factors, ACE-2, MCP-1, Micro RNA 146 gene expression, and COVID-19 infection and disease severity.
Methods: This study was carried out on 165 cases of COVID-19 and 138 controls. ACE2 and MCP1 levels were measured in COVID-19 cases and control by ELISA and micro-RNA 146 expression by PCR.
Results: We found an increased blood level of ACE2 and MCP1 in COVID- 19 patients more than healthy persons, significant down-regulation of micro-RNA 146 gene expression in cases than in controls. There was a significant correlation between increased blood level of ACE2, regulation of micro-RNA 146 gene expression and severity of lung affection, a significant correlation was found between increased blood level of MCP1 and thrombosis in COVID-19 patients. Neurological complications were significantly correlated with more viral load, more ACE2 blood level, and down regulation of micro RNA146 expression.
Conclusions: High viral load, increased blood level of ACE2, and down-regulation of micro-RNA 146 expression are associated with more severe lung injury and with the presence of neurologic complications like convulsions and coma in COVID-19 Egyptian patients.
Keywords: ACE2; COVID-19; MCP1; Thrombosis; Viruses; micro RNA 146.
. 2022 Aug 29.
doi: 10.2174/1871526522666220829153042. Online ahead of print.
Human ACE-2, MCP1 and micro-RNA 146 as Novel Markers for COVID- 19 Affection and Severity
Amal Ahmed Mohamed[SUP] 1 [/SUP], Sherief Abd-Elsalam[SUP] 2 [/SUP], Ahmed Abdelghani[SUP] 3 [/SUP], Mohamed Badr Hassan[SUP] 3 [/SUP], Doaa Ghaith[SUP] 4 [/SUP], Omnia Ezzat[SUP] 5 [/SUP], Dalia Ali El-Damasy[SUP] 6 [/SUP], Norhan Nagdi Madbouli[SUP] 7 [/SUP], Mohmoud Hamada[SUP] 8 [/SUP], Mohamed Abdel Khalik Hepatology Gastroenterology And Infectious Diseases[SUP] 9 [/SUP], Shaimaa M Al-Tabbakh[SUP] 10 [/SUP], Kareman Ahmed Ebrahim Eshra[SUP] 11 [/SUP], Nivin Baiomy[SUP] 12 [/SUP]
Affiliations
- PMID: 36043754
- DOI: 10.2174/1871526522666220829153042
Abstract
Background & aims: Coronavirus disease - 2019 (COVID-19) is a major pandemic that causes high morbidity and mortality rates.
Aim of this study: to detect the relations between many risk factors, ACE-2, MCP-1, Micro RNA 146 gene expression, and COVID-19 infection and disease severity.
Methods: This study was carried out on 165 cases of COVID-19 and 138 controls. ACE2 and MCP1 levels were measured in COVID-19 cases and control by ELISA and micro-RNA 146 expression by PCR.
Results: We found an increased blood level of ACE2 and MCP1 in COVID- 19 patients more than healthy persons, significant down-regulation of micro-RNA 146 gene expression in cases than in controls. There was a significant correlation between increased blood level of ACE2, regulation of micro-RNA 146 gene expression and severity of lung affection, a significant correlation was found between increased blood level of MCP1 and thrombosis in COVID-19 patients. Neurological complications were significantly correlated with more viral load, more ACE2 blood level, and down regulation of micro RNA146 expression.
Conclusions: High viral load, increased blood level of ACE2, and down-regulation of micro-RNA 146 expression are associated with more severe lung injury and with the presence of neurologic complications like convulsions and coma in COVID-19 Egyptian patients.
Keywords: ACE2; COVID-19; MCP1; Thrombosis; Viruses; micro RNA 146.