tetano
Editor, Senior Moderator
J Infect Chemother. 2013 Dec 11. pii: S1341-321X(13)00018-4. doi: 10.1016/j.jiac.2013.07.012. [Epub ahead of print]
Influence of prior pandemic A(H1N1)2009 virus infection on invasion of MDCK cells by community-associated methicillin-resistant Staphylococcus aureus.
Takayama Y1, Yano H2, Nojima Y3, Nakano R4, Okamoto R5, Hirakata Y2, Sunakawa K6, Akahoshi T7, Kaku M2.
Author information
Abstract
Secondary bacterial pneumonia due to community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has become a highly publicized cause of death associated with influenza. In this study, we performed the gentamicin-killing assay using Madin-Darby canine kidney (MDCK) cells and MRSA strains to investigate whether prior infection from pandemic A(H1N1)2009 virus (A[H1N1]pdm09) lead to increased invasion of MDCK cells by MRSA. We found that the invasion rate of two MRSA strains (ATCC BAA-1680 [USA 300] and ATCC BAA-1699 [USA 100]) into intact MDCK cell monolayers was 0.29 ? 0.15% and 0.007 ? 0.002%, respectively (p < 0.01, n ≥ 3). In addition, the relative invasion rate of both ATCC BAA-1680 and ATCC BAA-1699 was significantly increased by prior A(H1N1)pdm09 infection of MDCK monolayers from 1 ? 0.28 to 1.38 ? 0.02 and from 1 ? 0.24 to 1.73 ? 0.29, respectively (p < 0.01). These results indicate that ATCC BAA-1680 displays much stronger invasiveness of MDCK cells than ATCC BAA-1699, although invasion of both strains was increased by prior A(H1N1)pdm09 infection. In conclusion, this study provided the first evidence that prior A(H1N1)pdm09 infection facilitates the invasion of MDCK cells by MRSA, presumably due to cellular injury caused by the virus.
Copyright ? 2013. Published by Elsevier Ltd.
KEYWORDS:
A(H1N1)pdm09, Community-associated MRSA, Invasion
PMID:
24462431
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24462431
Influence of prior pandemic A(H1N1)2009 virus infection on invasion of MDCK cells by community-associated methicillin-resistant Staphylococcus aureus.
Takayama Y1, Yano H2, Nojima Y3, Nakano R4, Okamoto R5, Hirakata Y2, Sunakawa K6, Akahoshi T7, Kaku M2.
Author information
Abstract
Secondary bacterial pneumonia due to community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has become a highly publicized cause of death associated with influenza. In this study, we performed the gentamicin-killing assay using Madin-Darby canine kidney (MDCK) cells and MRSA strains to investigate whether prior infection from pandemic A(H1N1)2009 virus (A[H1N1]pdm09) lead to increased invasion of MDCK cells by MRSA. We found that the invasion rate of two MRSA strains (ATCC BAA-1680 [USA 300] and ATCC BAA-1699 [USA 100]) into intact MDCK cell monolayers was 0.29 ? 0.15% and 0.007 ? 0.002%, respectively (p < 0.01, n ≥ 3). In addition, the relative invasion rate of both ATCC BAA-1680 and ATCC BAA-1699 was significantly increased by prior A(H1N1)pdm09 infection of MDCK monolayers from 1 ? 0.28 to 1.38 ? 0.02 and from 1 ? 0.24 to 1.73 ? 0.29, respectively (p < 0.01). These results indicate that ATCC BAA-1680 displays much stronger invasiveness of MDCK cells than ATCC BAA-1699, although invasion of both strains was increased by prior A(H1N1)pdm09 infection. In conclusion, this study provided the first evidence that prior A(H1N1)pdm09 infection facilitates the invasion of MDCK cells by MRSA, presumably due to cellular injury caused by the virus.
Copyright ? 2013. Published by Elsevier Ltd.
KEYWORDS:
A(H1N1)pdm09, Community-associated MRSA, Invasion
PMID:
24462431
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24462431