tetano
Editor, Senior Moderator
J Biol Chem. 2012 Feb 16. [Epub ahead of print]
Influenza A virus induces Interleukin-27 through cyclooxygenase-2 and protein kinase A signaling.
Liu L, Cao Z, Chen J, Li R, Cao Y, Zhu C, Wu K, Wu J, Liu F, Zhu Y.
Source
Wuhan University, China.
Abstract
We previously reported that IL-27, which belongs to the IL-12 family of cytokines, is elevated in the serum of patients infected with influenza A virus (IAV). Here, we show that the expression of IL-27 was significantly upregulated in A549 human lung epithelial cells and human peripheral blood mononuclear cells (PBMCs) infected with IAV. Additionally, IAV triggered IL-27 expression through protein kinase A (PKA) and CREB signaling, which was mediated by cyclooxygenase-2-derived prostaglandin E2 (PGE2). IL-27 inhibited IAV replication by STAT1/2/3 phosphorylation and activated anti-viral factor protein kinase R (PKR) expression and phosphorylation. Clinical analysis showed that IL-27 levels were significantly elevated in a cohort of patients infected with IAV compared to healthy individuals and that circulating IL-27 levels were tightly and positively correlated with PGE2 levels. These results indicate that IL-27 expression is one host immune factor produced in response to IAV infection and that elevated IL-27 levels inhibit viral replication.
PMID:
22343630
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22343630
Influenza A virus induces Interleukin-27 through cyclooxygenase-2 and protein kinase A signaling.
Liu L, Cao Z, Chen J, Li R, Cao Y, Zhu C, Wu K, Wu J, Liu F, Zhu Y.
Source
Wuhan University, China.
Abstract
We previously reported that IL-27, which belongs to the IL-12 family of cytokines, is elevated in the serum of patients infected with influenza A virus (IAV). Here, we show that the expression of IL-27 was significantly upregulated in A549 human lung epithelial cells and human peripheral blood mononuclear cells (PBMCs) infected with IAV. Additionally, IAV triggered IL-27 expression through protein kinase A (PKA) and CREB signaling, which was mediated by cyclooxygenase-2-derived prostaglandin E2 (PGE2). IL-27 inhibited IAV replication by STAT1/2/3 phosphorylation and activated anti-viral factor protein kinase R (PKR) expression and phosphorylation. Clinical analysis showed that IL-27 levels were significantly elevated in a cohort of patients infected with IAV compared to healthy individuals and that circulating IL-27 levels were tightly and positively correlated with PGE2 levels. These results indicate that IL-27 expression is one host immune factor produced in response to IAV infection and that elevated IL-27 levels inhibit viral replication.
PMID:
22343630
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22343630