tetano
Editor, Senior Moderator
J Clin Lab Anal. 2018 Aug 11:e22638. doi: 10.1002/jcla.22638. [Epub ahead of print]
[h=1]Influenza A virus inhibits influenza virus replication by inducing IL-37.[/h] Zhou F[SUP]1,[/SUP][SUP]2[/SUP], Zhu CL[SUP]3[/SUP], Niu ZL[SUP]3[/SUP], Xu FX[SUP]4[/SUP], Song H[SUP]4[/SUP], Liu XH[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUNDS:[/h] The influenza virus is one of the major pathogens that seriously affect human health. It can cause a strong immune response and trigger a series of complications. Interleukin 37 (IL-37) is a newly discovered cytokine that plays an important regulatory role in infection and immunity. To date, there have been few studies on the correlation between influenza virus infection and IL-37.
[h=4]METHODS:[/h] Serum levels of IL-37 in 115 patients with influenza A virus (IAV) infection and 102 healthy subjects were measured by an enzyme-linked immunosorbent assay (ELISA). Real-time quantitative PCR (RT-qPCR) was used to detect differences in IL-37 expression in peripheral blood mononuclear cells (PBMCs) between IAV patients and healthy subjects. IL-37 expression was measured in A549 cells and PBMCs infected with IAV H3N2 using ELISA and RT-qPCR. After the H3N2-infected A549 cells were treated with human IL-37, the concentration of viral RNA was determined using RT-qPCR, and the titer of influenza virus was determined by a hemagglutination test.
[h=4]RESULTS:[/h] The IL-37 levels in the sera and PBMCs of patients infected with IAV were higher than those of healthy subjects. The expression of IL-37 mRNA and protein in IAV-infected A549 cells and PBMCs was upregulated, and IL-37 protein was able to inhibit the replication of IAV RNA.
[h=4]CONCLUSION:[/h] IAV-induced IL-37 expression inhibits IAV replication.
? 2018 Wiley Periodicals, Inc.
[h=4]KEYWORDS:[/h] influenza A virus; interleukin 37; replication
PMID: 30098064 DOI: 10.1002/jcla.22638
[h=1]Influenza A virus inhibits influenza virus replication by inducing IL-37.[/h] Zhou F[SUP]1,[/SUP][SUP]2[/SUP], Zhu CL[SUP]3[/SUP], Niu ZL[SUP]3[/SUP], Xu FX[SUP]4[/SUP], Song H[SUP]4[/SUP], Liu XH[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUNDS:[/h] The influenza virus is one of the major pathogens that seriously affect human health. It can cause a strong immune response and trigger a series of complications. Interleukin 37 (IL-37) is a newly discovered cytokine that plays an important regulatory role in infection and immunity. To date, there have been few studies on the correlation between influenza virus infection and IL-37.
[h=4]METHODS:[/h] Serum levels of IL-37 in 115 patients with influenza A virus (IAV) infection and 102 healthy subjects were measured by an enzyme-linked immunosorbent assay (ELISA). Real-time quantitative PCR (RT-qPCR) was used to detect differences in IL-37 expression in peripheral blood mononuclear cells (PBMCs) between IAV patients and healthy subjects. IL-37 expression was measured in A549 cells and PBMCs infected with IAV H3N2 using ELISA and RT-qPCR. After the H3N2-infected A549 cells were treated with human IL-37, the concentration of viral RNA was determined using RT-qPCR, and the titer of influenza virus was determined by a hemagglutination test.
[h=4]RESULTS:[/h] The IL-37 levels in the sera and PBMCs of patients infected with IAV were higher than those of healthy subjects. The expression of IL-37 mRNA and protein in IAV-infected A549 cells and PBMCs was upregulated, and IL-37 protein was able to inhibit the replication of IAV RNA.
[h=4]CONCLUSION:[/h] IAV-induced IL-37 expression inhibits IAV replication.
? 2018 Wiley Periodicals, Inc.
[h=4]KEYWORDS:[/h] influenza A virus; interleukin 37; replication
PMID: 30098064 DOI: 10.1002/jcla.22638