tetano
Editor, Senior Moderator
Virology. 2012 Jun 1. [Epub ahead of print]
Influenza M1 VLPs containing neuraminidase induce heterosubtypic cross-protection.
Quan FS, Kim MC, Lee BJ, Song JM, Compans RW, Kang SM.
Source
Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul 130-701, Korea.
Abstract
Influenza virus like particles (VLPs) containing hemagglutinin were previously demonstrated to induce protection against the homologous strains. However, little information is available on the protective role of neuraminidase (NA), the second major glycoprotein. In this study, we developed VLPs (NA VLPs) containing NA and M1 derived from A/PR/8/34 (H1N1) influenza virus, and investigated their ability to induce protective immunity. Intranasal immunization with NA VLPs induced serum antibody responses to H1N1 and H3N2 influenza A viruses as well as significant neuraminidase inhibition activity. Importantly, mice immunized with NA VLPs were 100% protected against lethal infection by the homologous A/PR/8/34 (H1N1) as well as heterosubtypic A/Philippines/82 (H3N2) virus, although body weight loss was observed after lethal challenge with heterosubtypic H3N2 virus. The present study therefore provides evidence that influenza VLPs containing M1 and NA are capable of inducing immunity to homologous as well as antigenically distinct influenza A virus strains.
Copyright ? 2012 Elsevier Inc. All rights reserved.
PMID:
22658901
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22658901
Influenza M1 VLPs containing neuraminidase induce heterosubtypic cross-protection.
Quan FS, Kim MC, Lee BJ, Song JM, Compans RW, Kang SM.
Source
Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul 130-701, Korea.
Abstract
Influenza virus like particles (VLPs) containing hemagglutinin were previously demonstrated to induce protection against the homologous strains. However, little information is available on the protective role of neuraminidase (NA), the second major glycoprotein. In this study, we developed VLPs (NA VLPs) containing NA and M1 derived from A/PR/8/34 (H1N1) influenza virus, and investigated their ability to induce protective immunity. Intranasal immunization with NA VLPs induced serum antibody responses to H1N1 and H3N2 influenza A viruses as well as significant neuraminidase inhibition activity. Importantly, mice immunized with NA VLPs were 100% protected against lethal infection by the homologous A/PR/8/34 (H1N1) as well as heterosubtypic A/Philippines/82 (H3N2) virus, although body weight loss was observed after lethal challenge with heterosubtypic H3N2 virus. The present study therefore provides evidence that influenza VLPs containing M1 and NA are capable of inducing immunity to homologous as well as antigenically distinct influenza A virus strains.
Copyright ? 2012 Elsevier Inc. All rights reserved.
PMID:
22658901
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22658901