tetano
Editor, Senior Moderator
Influenza Other Respir Viruses
. 2024 Dec;18(12):e70048.
doi: 10.1111/irv.70048. Rapid and Safe Neutralization Assay for Circulating H5N1 Influenza Virus in Dairy Cows
Kei Miyakawa[SUP] 1 2 3 [/SUP], Makoto Ota[SUP] 1 3 [/SUP], Kaori Sano[SUP] 1 [/SUP], Fumitaka Momose[SUP] 4 [/SUP], Takashi Okura[SUP] 5 [/SUP], Noriko Kishida[SUP] 1 [/SUP], Tomoko Arita[SUP] 1 [/SUP], Yasushi Suzuki[SUP] 1 [/SUP], Masayuki Shirakura[SUP] 1 [/SUP], Hideki Asanuma[SUP] 1 [/SUP], Shinji Watanabe[SUP] 1 [/SUP], Akihide Ryo[SUP] 3 5 [/SUP], Hideki Hasegawa[SUP] 1 [/SUP]
Affiliations
Rapid and safe neutralization assays are required for highly pathogenic avian influenza viruses, including a clade 2.3.4.4b H5N1 subtype recently found in cows. Here, we report a neutralization assay using luminescent virus-like particles. This assay has lower biosafety requirements and provides a larger dynamic range than conventional methods. We applied this technique to evaluate the cross-reactivity of neutralizing antibodies induced by clade 2.3.4.4b candidate vaccine viruses (CVVs) with the cow-derived H5N1 virus. Our findings indicate that these CVVs share antigenic characteristics with the cow-derived H5N1 virus, suggesting the potential efficacy of vaccines developed using these CVVs.
Keywords: H5N1; dairy cows; hiVLP; neutralization assay.
. 2024 Dec;18(12):e70048.
doi: 10.1111/irv.70048. Rapid and Safe Neutralization Assay for Circulating H5N1 Influenza Virus in Dairy Cows
Kei Miyakawa[SUP] 1 2 3 [/SUP], Makoto Ota[SUP] 1 3 [/SUP], Kaori Sano[SUP] 1 [/SUP], Fumitaka Momose[SUP] 4 [/SUP], Takashi Okura[SUP] 5 [/SUP], Noriko Kishida[SUP] 1 [/SUP], Tomoko Arita[SUP] 1 [/SUP], Yasushi Suzuki[SUP] 1 [/SUP], Masayuki Shirakura[SUP] 1 [/SUP], Hideki Asanuma[SUP] 1 [/SUP], Shinji Watanabe[SUP] 1 [/SUP], Akihide Ryo[SUP] 3 5 [/SUP], Hideki Hasegawa[SUP] 1 [/SUP]
Affiliations
- PMID: 39618269
- PMCID: PMC11609488
- DOI: 10.1111/irv.70048
Rapid and safe neutralization assays are required for highly pathogenic avian influenza viruses, including a clade 2.3.4.4b H5N1 subtype recently found in cows. Here, we report a neutralization assay using luminescent virus-like particles. This assay has lower biosafety requirements and provides a larger dynamic range than conventional methods. We applied this technique to evaluate the cross-reactivity of neutralizing antibodies induced by clade 2.3.4.4b candidate vaccine viruses (CVVs) with the cow-derived H5N1 virus. Our findings indicate that these CVVs share antigenic characteristics with the cow-derived H5N1 virus, suggesting the potential efficacy of vaccines developed using these CVVs.
Keywords: H5N1; dairy cows; hiVLP; neutralization assay.