tetano
Editor, Senior Moderator
Influenza Other Respir Viruses. 2017 Jul 28. doi: 10.1111/irv.12472. [Epub ahead of print]
[h=1]Influenza virus-like particles harboring H9N2 HA and NA proteins induce a protective immune response in chicken.[/h] Li X[SUP]1[/SUP], Ju H[SUP]1[/SUP], Liu J[SUP]1[/SUP], Yang D[SUP]1[/SUP], Qi X[SUP]1[/SUP], Yang X[SUP]1[/SUP], Qiu Y[SUP]2[/SUP], Zheng J[SUP]3[/SUP], Ge F[SUP]1[/SUP], Zhou J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Avian influenza viruses represent a growing threat of an influenza pandemic. The co-circulation of multiple H9N2 genotypes over the past decade has been replaced by one predominant genotype-G57 genotype, which displays a changed antigenicity and improved adaptability in chickens. Effective H9N2 subtype avian influenza virus vaccines for poultry are urgently needed.
[h=4]OBJECTIVE:[/h] In this study, we constructed H9N2 subtype avian influenza virus-like particle (VLP) and evaluated its protective efficacy in specific pathogen-free (SPF) chickens to lay the foundation for developing an effective vaccine against influenza viruses.
[h=4]METHODS:[/h] Expression of influenza proteins in VLPs was confirmed by Western blot, hemagglutination inhibition (HI) and neuraminidase inhibition (NI). The morphology was observed by electron microscopy. A group of 15 three-week-old SPF chickens was divided into three sub-groups of five chickens immunized with VLP, commercial vaccine and PBS. Challenge study was performed to evaluate efficacy of VLP vaccine.
[h=4]RESULTS AND CONCLUSIONS:[/h] The hemagglutinin (HA) and neuraminidase (NA) proteins were co-expressed in the infected cells, self-assembled, and were released into the culture medium in the form of VLPs of diameter ~80 nm. The VLPs exhibited some functional characteristics of a full influenza virus, including hemagglutination and neuraminidase activity. In SPF chickens, the VLPs elicited serum antibodies specific for H9N2, and induced a higher HI titer (as detected by a homologous antigen) than did a commercial H9N2 vaccine (A/chicken/Shanghai/F/1998). Viral shedding from VLP vaccine sub-group was reduced compared with commercial vaccine sub-group and control sub-group. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] VLP ; Chickens; H9N2; Vaccine
PMID: 28752641 DOI: 10.1111/irv.12472
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[h=1]Influenza virus-like particles harboring H9N2 HA and NA proteins induce a protective immune response in chicken.[/h] Li X[SUP]1[/SUP], Ju H[SUP]1[/SUP], Liu J[SUP]1[/SUP], Yang D[SUP]1[/SUP], Qi X[SUP]1[/SUP], Yang X[SUP]1[/SUP], Qiu Y[SUP]2[/SUP], Zheng J[SUP]3[/SUP], Ge F[SUP]1[/SUP], Zhou J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Avian influenza viruses represent a growing threat of an influenza pandemic. The co-circulation of multiple H9N2 genotypes over the past decade has been replaced by one predominant genotype-G57 genotype, which displays a changed antigenicity and improved adaptability in chickens. Effective H9N2 subtype avian influenza virus vaccines for poultry are urgently needed.
[h=4]OBJECTIVE:[/h] In this study, we constructed H9N2 subtype avian influenza virus-like particle (VLP) and evaluated its protective efficacy in specific pathogen-free (SPF) chickens to lay the foundation for developing an effective vaccine against influenza viruses.
[h=4]METHODS:[/h] Expression of influenza proteins in VLPs was confirmed by Western blot, hemagglutination inhibition (HI) and neuraminidase inhibition (NI). The morphology was observed by electron microscopy. A group of 15 three-week-old SPF chickens was divided into three sub-groups of five chickens immunized with VLP, commercial vaccine and PBS. Challenge study was performed to evaluate efficacy of VLP vaccine.
[h=4]RESULTS AND CONCLUSIONS:[/h] The hemagglutinin (HA) and neuraminidase (NA) proteins were co-expressed in the infected cells, self-assembled, and were released into the culture medium in the form of VLPs of diameter ~80 nm. The VLPs exhibited some functional characteristics of a full influenza virus, including hemagglutination and neuraminidase activity. In SPF chickens, the VLPs elicited serum antibodies specific for H9N2, and induced a higher HI titer (as detected by a homologous antigen) than did a commercial H9N2 vaccine (A/chicken/Shanghai/F/1998). Viral shedding from VLP vaccine sub-group was reduced compared with commercial vaccine sub-group and control sub-group. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] VLP ; Chickens; H9N2; Vaccine
PMID: 28752641 DOI: 10.1111/irv.12472
Free full text