tetano
Editor, Senior Moderator
Int Immunopharmacol
. 2020 Jul 30;88:106861.
doi: 10.1016/j.intimp.2020.106861. Online ahead of print.
Serological detection of 2019-nCoV respond to the epidemic: A useful complement to nucleic acid testing
Jin Zhang[SUP] 1 [/SUP], Xiaoming Zhang[SUP] 2 [/SUP], Jianhua Liu[SUP] 1 [/SUP], Yuan Ban[SUP] 3 [/SUP], Na Li[SUP] 1 [/SUP], Yunhai Wu[SUP] 3 [/SUP], Yong Liu[SUP] 1 [/SUP], Rui Ye[SUP] 4 [/SUP], Jinyang Liu[SUP] 5 [/SUP], Xinghai Li[SUP] 5 [/SUP], Luping Li[SUP] 2 [/SUP], Xiaosong Qin[SUP] 1 [/SUP], Rui Zheng[SUP] 6 [/SUP]
Affiliations
Abstract
Corona Virus Disease 2019 (COVID-19) has spread rapidly to more than 215 countries, with over 11.91 million reported cases and more than 540,000 deaths. Rapid diagnosis remains a bottleneck for containing the epidemic. We used an automated chemiluminescent immunoassay to detect serum IgM and IgG antibodies to the 2019-nCoV in 742 subjects, so as to observe the dynamic process of antibody production in COVID-19 disease and seroepidemiology in different populations. Patients with COVID-19 were reactive (positive) for specific antibodies within 3-15 days after onset of symptoms. Specific IgM and IgG levels increased with the progression of the disease. The areas under the receiver operating characteristic curves for IgM and IgG were 0.984 and 1.000, respectively. This antibody detection assay had good sensitivity and specificity. The understanding of the dynamic serological changes of COVID-19 patients and the seroepidemiological situation of the population will be helpful to further control the epidemic of COVID-19.
Keywords: Antibody; Coronavirus; Nucleic acid; Serology.
. 2020 Jul 30;88:106861.
doi: 10.1016/j.intimp.2020.106861. Online ahead of print.
Serological detection of 2019-nCoV respond to the epidemic: A useful complement to nucleic acid testing
Jin Zhang[SUP] 1 [/SUP], Xiaoming Zhang[SUP] 2 [/SUP], Jianhua Liu[SUP] 1 [/SUP], Yuan Ban[SUP] 3 [/SUP], Na Li[SUP] 1 [/SUP], Yunhai Wu[SUP] 3 [/SUP], Yong Liu[SUP] 1 [/SUP], Rui Ye[SUP] 4 [/SUP], Jinyang Liu[SUP] 5 [/SUP], Xinghai Li[SUP] 5 [/SUP], Luping Li[SUP] 2 [/SUP], Xiaosong Qin[SUP] 1 [/SUP], Rui Zheng[SUP] 6 [/SUP]
Affiliations
- PMID: 32771946
- DOI: 10.1016/j.intimp.2020.106861
Abstract
Corona Virus Disease 2019 (COVID-19) has spread rapidly to more than 215 countries, with over 11.91 million reported cases and more than 540,000 deaths. Rapid diagnosis remains a bottleneck for containing the epidemic. We used an automated chemiluminescent immunoassay to detect serum IgM and IgG antibodies to the 2019-nCoV in 742 subjects, so as to observe the dynamic process of antibody production in COVID-19 disease and seroepidemiology in different populations. Patients with COVID-19 were reactive (positive) for specific antibodies within 3-15 days after onset of symptoms. Specific IgM and IgG levels increased with the progression of the disease. The areas under the receiver operating characteristic curves for IgM and IgG were 0.984 and 1.000, respectively. This antibody detection assay had good sensitivity and specificity. The understanding of the dynamic serological changes of COVID-19 patients and the seroepidemiological situation of the population will be helpful to further control the epidemic of COVID-19.
Keywords: Antibody; Coronavirus; Nucleic acid; Serology.