tetano
Editor, Senior Moderator
Int J Environ Res Public Health
. 2020 Jul 30;17(15):E5508.
doi: 10.3390/ijerph17155508.
Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic
Hana Mlejnkova[SUP] 1 [/SUP], Katerina Sovova[SUP] 2 [/SUP], Petra Vasickova[SUP] 3 [/SUP], Vera Ocenaskova[SUP] 1 [/SUP], Lucie Jasikova[SUP] 1 [/SUP], Eva Juranova[SUP] 1 [/SUP]
Affiliations
Abstract
The virus SARS-CoV-2, which has caused the recent COVID-19 pandemic, may be present in the stools of COVID-19 patients. Therefore, we aimed to detect SARS-CoV-2 in wastewater for surveillance of SARS-CoV-2 in the population. Samples of untreated wastewater were collected from 33 wastewater treatment plants (WWTPs) of different sizes within the Czech Republic. SARS-CoV-2 RNA was concentrated from wastewater and viral RNA was determined using real-time reverse transcription polymerase chain reaction (RT-qPCR). SARS-CoV-2 RNA was detected in 11.6% of samples and more than 27.3% of WWTPs; in some of them, SARS-CoV-2 was detected repeatedly. Our preliminary results indicate that an epidemiology approach that focuses on the determination of SARS-CoV-2 in wastewater could be suitable for SARS-CoV-2 surveillance in the population.
Keywords: RT-qPCR; SARS-CoV-2; coronavirus; early warning system; epidemic; virus; wastewater; wastewater-based epidemiology.
. 2020 Jul 30;17(15):E5508.
doi: 10.3390/ijerph17155508.
Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic
Hana Mlejnkova[SUP] 1 [/SUP], Katerina Sovova[SUP] 2 [/SUP], Petra Vasickova[SUP] 3 [/SUP], Vera Ocenaskova[SUP] 1 [/SUP], Lucie Jasikova[SUP] 1 [/SUP], Eva Juranova[SUP] 1 [/SUP]
Affiliations
- PMID: 32751749
- DOI: 10.3390/ijerph17155508
Abstract
The virus SARS-CoV-2, which has caused the recent COVID-19 pandemic, may be present in the stools of COVID-19 patients. Therefore, we aimed to detect SARS-CoV-2 in wastewater for surveillance of SARS-CoV-2 in the population. Samples of untreated wastewater were collected from 33 wastewater treatment plants (WWTPs) of different sizes within the Czech Republic. SARS-CoV-2 RNA was concentrated from wastewater and viral RNA was determined using real-time reverse transcription polymerase chain reaction (RT-qPCR). SARS-CoV-2 RNA was detected in 11.6% of samples and more than 27.3% of WWTPs; in some of them, SARS-CoV-2 was detected repeatedly. Our preliminary results indicate that an epidemiology approach that focuses on the determination of SARS-CoV-2 in wastewater could be suitable for SARS-CoV-2 surveillance in the population.
Keywords: RT-qPCR; SARS-CoV-2; coronavirus; early warning system; epidemic; virus; wastewater; wastewater-based epidemiology.