tetano
Editor, Senior Moderator
Int J Infect Dis
. 2020 Jun 26;S1201-9712(20)30509-9.
doi: 10.1016/j.ijid.2020.06.074. Online ahead of print.
Efficacy of a Novel SARS-CoV-2 Detection Kit Without RNA Extraction and Purification
Tatsuya Fukumoto[SUP] 1 [/SUP], Sumio Iwasaki[SUP] 1 [/SUP], Shinichi Fujisawa[SUP] 1 [/SUP], Kasumi Hayasaka[SUP] 1 [/SUP], Kaori Sato[SUP] 1 [/SUP], Satoshi Oguri[SUP] 1 [/SUP], Keisuke Taki[SUP] 1 [/SUP], Sho Nakakubo[SUP] 2 [/SUP], Keisuke Kamada[SUP] 2 [/SUP], Yu Yamashita[SUP] 2 [/SUP], Satoshi Konno[SUP] 2 [/SUP], Mutsumi Nishida[SUP] 1 [/SUP], Junichi Sugita[SUP] 1 [/SUP], Takanori Teshima[SUP] 3 [/SUP]
AffiliationsExpand
Abstract
Rapid detection of SARS-CoV-2 is critical for the diagnosis of coronavirus disease 2019 (COVID-19) and preventing the spread of the virus. A novel "2019 Novel Coronavirus Detection Kit (nCoV-DK)" halves detection time by eliminating the steps of RNA extraction and purification. We evaluated concordance between the nCoV-DK and direct PCR. The virus was detected in 53/71 (74.6%) and 55/71 (77.5%) by the direct PCR and nCoV-DK, respectively, with overall concordance rate of 94.4%: 95.2% in nasopharyngeal swab, 95.5% in saliva, and 85.7% in sputum. The nCoV-DK effectively detects SARS-CoV-2 in all types of the samples including saliva, while reducing time required for detection, labor, and risk of human error.
Keywords: COVID-19; PCR; SARS-CoV-2; Saliva.
. 2020 Jun 26;S1201-9712(20)30509-9.
doi: 10.1016/j.ijid.2020.06.074. Online ahead of print.
Efficacy of a Novel SARS-CoV-2 Detection Kit Without RNA Extraction and Purification
Tatsuya Fukumoto[SUP] 1 [/SUP], Sumio Iwasaki[SUP] 1 [/SUP], Shinichi Fujisawa[SUP] 1 [/SUP], Kasumi Hayasaka[SUP] 1 [/SUP], Kaori Sato[SUP] 1 [/SUP], Satoshi Oguri[SUP] 1 [/SUP], Keisuke Taki[SUP] 1 [/SUP], Sho Nakakubo[SUP] 2 [/SUP], Keisuke Kamada[SUP] 2 [/SUP], Yu Yamashita[SUP] 2 [/SUP], Satoshi Konno[SUP] 2 [/SUP], Mutsumi Nishida[SUP] 1 [/SUP], Junichi Sugita[SUP] 1 [/SUP], Takanori Teshima[SUP] 3 [/SUP]
AffiliationsExpand
- PMID: 32599282
- DOI: 10.1016/j.ijid.2020.06.074
Abstract
Rapid detection of SARS-CoV-2 is critical for the diagnosis of coronavirus disease 2019 (COVID-19) and preventing the spread of the virus. A novel "2019 Novel Coronavirus Detection Kit (nCoV-DK)" halves detection time by eliminating the steps of RNA extraction and purification. We evaluated concordance between the nCoV-DK and direct PCR. The virus was detected in 53/71 (74.6%) and 55/71 (77.5%) by the direct PCR and nCoV-DK, respectively, with overall concordance rate of 94.4%: 95.2% in nasopharyngeal swab, 95.5% in saliva, and 85.7% in sputum. The nCoV-DK effectively detects SARS-CoV-2 in all types of the samples including saliva, while reducing time required for detection, labor, and risk of human error.
Keywords: COVID-19; PCR; SARS-CoV-2; Saliva.