tetano
Editor, Senior Moderator
Int J Infect Dis
. 2023 Nov 18:S1201-9712(23)00769-5.
doi: 10.1016/j.ijid.2023.11.008. Online ahead of print. Pre-existing cross-reactive neutralizing activity against SARS-CoV-2 and seasonal coronaviruses prior to the COVID-19 pandemic (2014-2019) with limited immunity against recent emerging SARS-CoV-2 variants, Vietnam
Thi Thanh Ngan Nguyen[SUP] 1 [/SUP], Ee Mei Choo[SUP] 2 [/SUP], Yukio Nakamura[SUP] 3 [/SUP], Ryuji Suzuki[SUP] 3 [/SUP], Takashi Shiina[SUP] 4 [/SUP], Tadasu Shin-I[SUP] 3 [/SUP], Mizuki Fukuta[SUP] 5 [/SUP], Co Thach Nguyen[SUP] 6 [/SUP], Thi Thu Thuy Nguyen[SUP] 6 [/SUP], Le Khanh Hang Nguyen[SUP] 6 [/SUP], Vu Mai Phuong Hoang[SUP] 6 [/SUP], Kouichi Morita[SUP] 5 [/SUP], Duc Anh Dang[SUP] 6 [/SUP], Futoshi Hasebe[SUP] 7 [/SUP], Thi Quynh Mai Le[SUP] 6 [/SUP], Meng Ling Moi[SUP] 8 [/SUP]
Affiliations
Objective: SARS-CoV-2 transmission and epidemic potential is related to the population' immunity levels. As such, assessing different regions' preexisting immune response to SARS-CoV-2 is important to understand the transmission potential of emerging SARS-CoV-2 variants.
Design: In 975 serum samples from Vietnam (2014 to 2019), anti-SARS-CoV-2 IgG levels were determined by ELISA. Plaque reduction neutralization test (PRNT) was performed using Wuhan strain and variants of concern (VOCs). Cross-reactivity was confirmed by analyzing B-cell receptor (BCR) repertoire sequences and identifying BCR repertoire sequences-derived T-cell epitopes.
Results: Overall, 20.9% (n=76/364) and 9.2% (n=7) demonstrated SARS-CoV-2 neutralizing activity (PRNT[SUB]50[/SUB]) against the Wuhan and alpha strain, respectively. Neutralizing activity against beta, gamma, and delta strains was absent (PRNT[SUB]50[/SUB]<5) in all samples. Cross-reactive epitopes against SARS-CoV-2 and other coronaviruses spike proteins were detected in the N-terminal domain (NTD), S2, and RBD regions.
Conclusion: Following BCR and major histocompatibility complex analysis, TCR-recognized epitope motif (TREM) among pathogenic coronaviruses and coronaviruses spike proteins were the top TREM peptide, suggesting that pre-existing immunity against SARS-CoV-2 in Vietnam was due to exposure to common cold coronaviruses. With limited immunity against emerging VOCs, further monitoring and control of the epidemic, along with COVID-19 vaccine programs against VOCs, are necessary.
Keywords: COVID-19; Vietnam; cellular immunity; pre-existing antibodies; variants of concern.
. 2023 Nov 18:S1201-9712(23)00769-5.
doi: 10.1016/j.ijid.2023.11.008. Online ahead of print. Pre-existing cross-reactive neutralizing activity against SARS-CoV-2 and seasonal coronaviruses prior to the COVID-19 pandemic (2014-2019) with limited immunity against recent emerging SARS-CoV-2 variants, Vietnam
Thi Thanh Ngan Nguyen[SUP] 1 [/SUP], Ee Mei Choo[SUP] 2 [/SUP], Yukio Nakamura[SUP] 3 [/SUP], Ryuji Suzuki[SUP] 3 [/SUP], Takashi Shiina[SUP] 4 [/SUP], Tadasu Shin-I[SUP] 3 [/SUP], Mizuki Fukuta[SUP] 5 [/SUP], Co Thach Nguyen[SUP] 6 [/SUP], Thi Thu Thuy Nguyen[SUP] 6 [/SUP], Le Khanh Hang Nguyen[SUP] 6 [/SUP], Vu Mai Phuong Hoang[SUP] 6 [/SUP], Kouichi Morita[SUP] 5 [/SUP], Duc Anh Dang[SUP] 6 [/SUP], Futoshi Hasebe[SUP] 7 [/SUP], Thi Quynh Mai Le[SUP] 6 [/SUP], Meng Ling Moi[SUP] 8 [/SUP]
Affiliations
- PMID: 37984763
- DOI: 10.1016/j.ijid.2023.11.008
Objective: SARS-CoV-2 transmission and epidemic potential is related to the population' immunity levels. As such, assessing different regions' preexisting immune response to SARS-CoV-2 is important to understand the transmission potential of emerging SARS-CoV-2 variants.
Design: In 975 serum samples from Vietnam (2014 to 2019), anti-SARS-CoV-2 IgG levels were determined by ELISA. Plaque reduction neutralization test (PRNT) was performed using Wuhan strain and variants of concern (VOCs). Cross-reactivity was confirmed by analyzing B-cell receptor (BCR) repertoire sequences and identifying BCR repertoire sequences-derived T-cell epitopes.
Results: Overall, 20.9% (n=76/364) and 9.2% (n=7) demonstrated SARS-CoV-2 neutralizing activity (PRNT[SUB]50[/SUB]) against the Wuhan and alpha strain, respectively. Neutralizing activity against beta, gamma, and delta strains was absent (PRNT[SUB]50[/SUB]<5) in all samples. Cross-reactive epitopes against SARS-CoV-2 and other coronaviruses spike proteins were detected in the N-terminal domain (NTD), S2, and RBD regions.
Conclusion: Following BCR and major histocompatibility complex analysis, TCR-recognized epitope motif (TREM) among pathogenic coronaviruses and coronaviruses spike proteins were the top TREM peptide, suggesting that pre-existing immunity against SARS-CoV-2 in Vietnam was due to exposure to common cold coronaviruses. With limited immunity against emerging VOCs, further monitoring and control of the epidemic, along with COVID-19 vaccine programs against VOCs, are necessary.
Keywords: COVID-19; Vietnam; cellular immunity; pre-existing antibodies; variants of concern.