tetano
Editor, Senior Moderator
iScience
. 2022 Jan 30;103830.
doi: 10.1016/j.isci.2022.103830. Online ahead of print.
CRISPR-Cas3-based diagnostics for SARS-CoV-2 and influenza virus
Kazuto Yoshimi[SUP] 1 2 [/SUP], Kohei Takeshita[SUP] 3 [/SUP], Seiya Yamayoshi[SUP] 4 [/SUP], Satomi Shibumura[SUP] 5 [/SUP], Yuko Yamauchi[SUP] 1 [/SUP], Masaki Yamamoto[SUP] 3 [/SUP], Hiroshi Yotsuyanagi[SUP] 6 [/SUP], Yoshihiro Kawaoka[SUP] 4 7 [/SUP], Tomoji Mashimo[SUP] 1 2 [/SUP]
Affiliations
Abstract
CRISPR-based diagnostics (CRISPR-dx), including the Cas12-based DETECTR and Cas13-based SHERLOCK Class 2 CRISPRs, have been used to detect the presence of DNA or RNA from pathogens, such as the 2009 pandemic influenza virus A (IAV) and the 2019 novel coronavirus SARS-CoV-2. Here, we describe the collateral single-stranded DNA cleavage with Class 1 type I CRISPR-Cas3 and highlight its potential for development as a Cas3-mediated rapid (within 40 min), low-cost, instrument-free detection method for SARS-CoV-2. This assay, which we call Cas3-Operated Nucleic Acid detectioN (CONAN), not only detects SARS-CoV-2 in clinical samples, but also offers specific detection of single-base-pair mutations in IAV variants. This tool allows rapid and accurate point-of-care testing for patients with suspected SARS-CoV-2 or drug-resistant IAV infections in hospitals.
. 2022 Jan 30;103830.
doi: 10.1016/j.isci.2022.103830. Online ahead of print.
CRISPR-Cas3-based diagnostics for SARS-CoV-2 and influenza virus
Kazuto Yoshimi[SUP] 1 2 [/SUP], Kohei Takeshita[SUP] 3 [/SUP], Seiya Yamayoshi[SUP] 4 [/SUP], Satomi Shibumura[SUP] 5 [/SUP], Yuko Yamauchi[SUP] 1 [/SUP], Masaki Yamamoto[SUP] 3 [/SUP], Hiroshi Yotsuyanagi[SUP] 6 [/SUP], Yoshihiro Kawaoka[SUP] 4 7 [/SUP], Tomoji Mashimo[SUP] 1 2 [/SUP]
Affiliations
- PMID: 35128347
- PMCID: PMC8801231
- DOI: 10.1016/j.isci.2022.103830
Abstract
CRISPR-based diagnostics (CRISPR-dx), including the Cas12-based DETECTR and Cas13-based SHERLOCK Class 2 CRISPRs, have been used to detect the presence of DNA or RNA from pathogens, such as the 2009 pandemic influenza virus A (IAV) and the 2019 novel coronavirus SARS-CoV-2. Here, we describe the collateral single-stranded DNA cleavage with Class 1 type I CRISPR-Cas3 and highlight its potential for development as a Cas3-mediated rapid (within 40 min), low-cost, instrument-free detection method for SARS-CoV-2. This assay, which we call Cas3-Operated Nucleic Acid detectioN (CONAN), not only detects SARS-CoV-2 in clinical samples, but also offers specific detection of single-base-pair mutations in IAV variants. This tool allows rapid and accurate point-of-care testing for patients with suspected SARS-CoV-2 or drug-resistant IAV infections in hospitals.