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iScience . Distinct T cell polyfunctional profile in SARS-CoV-2 seronegative children associated with endemic human coronavirus cross-reactivity

tetano

Editor, Senior Moderator
iScience


. 2023 Dec 14;27(1):108728.
doi: 10.1016/j.isci.2023.108728. eCollection 2024 Jan 19. Distinct T cell polyfunctional profile in SARS-CoV-2 seronegative children associated with endemic human coronavirus cross-reactivity

Ntombi Benede[SUP] 1 2 [/SUP], Marius B Tincho[SUP] 1 2 [/SUP], Avril Walters[SUP] 1 2 [/SUP], Vennesa Subbiah[SUP] 1 2 [/SUP], Amkele Ngomti[SUP] 1 2 [/SUP], Richard Baguma[SUP] 1 2 [/SUP], Claire Butters[SUP] 3 [/SUP], Lina Hahnle[SUP] 4 [/SUP], Mathilda Mennen[SUP] 4 [/SUP], Sango Skelem[SUP] 4 [/SUP], Marguerite Adriaanse[SUP] 4 [/SUP], Heidi Facey-Thomas[SUP] 5 [/SUP], Christiaan Scott[SUP] 3 [/SUP], Jonathan Day[SUP] 3 [/SUP], Timothy F Spracklen[SUP] 5 6 7 [/SUP], Strauss van Graan[SUP] 8 9 [/SUP], Sashkia R Balla[SUP] 8 9 [/SUP], Thandeka Moyo-Gwete[SUP] 8 9 [/SUP], Penny L Moore[SUP] 1 8 9 10 [/SUP], Rae MacGinty[SUP] 5 11 [/SUP], Maresa Botha[SUP] 5 11 [/SUP], Lesley Workman[SUP] 5 11 [/SUP], Marina Johnson[SUP] 12 [/SUP], David Goldblatt[SUP] 12 [/SUP], Heather J Zar[SUP] 1 5 11 13 [/SUP], Ntobeko A B Ntusi[SUP] 1 4 6 13 [/SUP], Liesl Zühlke[SUP] 1 5 6 7 [/SUP], Kate Webb[SUP] 3 14 [/SUP], Catherine Riou[SUP] 1 2 13 [/SUP], Wendy A Burgers[SUP] 1 2 13 [/SUP], Roanne S Keeton[SUP] 1 2 [/SUP]



Affiliations
Abstract

SARS-CoV-2 infection in children typically results in asymptomatic or mild disease. There is a paucity of studies on SARS-CoV-2 antiviral immunity in African children. We investigated SARS-CoV-2-specific T cell responses in 71 unvaccinated asymptomatic South African children who were seropositive or seronegative for SARS-CoV-2. SARS-CoV-2-specific CD4[SUP]+[/SUP] T cell responses were detectable in 83% of seropositive and 60% of seronegative children. Although the magnitude of the CD4[SUP]+[/SUP] T cell response did not differ significantly between the two groups, their functional profiles were distinct, with SARS-CoV-2 seropositive children exhibiting a higher proportion of polyfunctional T cells compared to their seronegative counterparts. The frequency of SARS-CoV-2-specific CD4[SUP]+[/SUP] T cells in seronegative children was associated with the endemic human coronavirus (HCoV) HKU1 IgG response. Overall, the presence of SARS-CoV-2-responding T cells in seronegative children may result from cross-reactivity to endemic coronaviruses and could contribute to the relative protection from disease observed in SARS-CoV-2-infected children.

Keywords: Components of the immune system; Immune response; Immunology; Virology.

 
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