tetano
Editor, Senior Moderator
iScience
. 2022 Dec 28;105887.
doi: 10.1016/j.isci.2022.105887. Online ahead of print.
Nonstructural protein 1 (nsp1) widespread RNA decay phenotype varies among Coronaviruses
Yahaira Bermudez, Jacob Miles, Mandy Muller
Abstract
Extensive remodeling of host gene expression by coronaviruses nsp1 proteins is a well-documented and conserved aspect of coronavirus-host takeover. Using comparative transcriptomics we investigated the diversity of transcriptional targets between various nsp1 proteins. Additionally, Affinity Purification followed by Mass-Spectrometry was implemented to identify common interactors between the different nsp1. While we detected widespread RNA destabilization, closely related nsp1 showed little similarities in clustering of targeted genes. We observed a partial overlap in transcriptional targeting between α-CoV 229E and MERS nsp1 which may suggest a common targeting mechanism, as MERS nsp1 preferentially targets nuclear transcripts. Our interactome data shows great variability between nsp1 interactions, with 229E nsp1, the smallest nsp1 tested here, interacting with the most host proteins. While nsp1 is a rather well-conserved protein with conserved functions across different coronaviruses, our data indicates that its precise effects on the host cell is virus-specific.
. 2022 Dec 28;105887.
doi: 10.1016/j.isci.2022.105887. Online ahead of print.
Nonstructural protein 1 (nsp1) widespread RNA decay phenotype varies among Coronaviruses
Yahaira Bermudez, Jacob Miles, Mandy Muller
- PMID: 36590901
- PMCID: PMC9794394
- DOI: 10.1016/j.isci.2022.105887
Abstract
Extensive remodeling of host gene expression by coronaviruses nsp1 proteins is a well-documented and conserved aspect of coronavirus-host takeover. Using comparative transcriptomics we investigated the diversity of transcriptional targets between various nsp1 proteins. Additionally, Affinity Purification followed by Mass-Spectrometry was implemented to identify common interactors between the different nsp1. While we detected widespread RNA destabilization, closely related nsp1 showed little similarities in clustering of targeted genes. We observed a partial overlap in transcriptional targeting between α-CoV 229E and MERS nsp1 which may suggest a common targeting mechanism, as MERS nsp1 preferentially targets nuclear transcripts. Our interactome data shows great variability between nsp1 interactions, with 229E nsp1, the smallest nsp1 tested here, interacting with the most host proteins. While nsp1 is a rather well-conserved protein with conserved functions across different coronaviruses, our data indicates that its precise effects on the host cell is virus-specific.