tetano
Editor, Senior Moderator
iScience
. 2023 Dec 13;27(1):108719.
doi: 10.1016/j.isci.2023.108719. eCollection 2024 Jan 19. SARS-CoV-2 produces a microRNA CoV2-miR-O8 in patients with COVID-19 infection
Elise J Tucker[SUP] 1 2 [/SUP], Soon Wei Wong[SUP] 1 2 [/SUP], Shashikanth Marri[SUP] 2 [/SUP], Saira Ali[SUP] 1 2 [/SUP], Anthony O Fedele[SUP] 1 [/SUP], Michael Z Michael[SUP] 2 3 [/SUP], Darling Rojas-Canales[SUP] 1 2 [/SUP], Jordan Y Li[SUP] 1 2 [/SUP], Chuan Kok Lim[SUP] 4 [/SUP], Jonathan M Gleadle[SUP] 1 2 [/SUP]
Affiliations
Many viruses produce microRNAs (miRNAs), termed viral miRNAs (v-miRNAs), with the capacity to target host gene expression. Bioinformatic and cell culture studies suggest that SARS-CoV-2 can also generate v-miRNAs. This patient-based study defines the SARS-CoV-2 encoded small RNAs present in nasopharyngeal swabs of patients with COVID-19 infection using small RNA-seq. A specific conserved sequence (CoV2-miR-O8) is defined that is not expressed in other coronaviruses but is preserved in all SARS-CoV-2 variants. CoV2-miR-O8 is highly represented in nasopharyngeal samples from patients with COVID-19 infection, is detected by RT-PCR assays in patients, has features consistent with Dicer and Drosha generation as well as interaction with Argonaute and targets specific human microRNAs.
Keywords: Health sciences; Immunology; Medical specialty; Medicine; Virology.
. 2023 Dec 13;27(1):108719.
doi: 10.1016/j.isci.2023.108719. eCollection 2024 Jan 19. SARS-CoV-2 produces a microRNA CoV2-miR-O8 in patients with COVID-19 infection
Elise J Tucker[SUP] 1 2 [/SUP], Soon Wei Wong[SUP] 1 2 [/SUP], Shashikanth Marri[SUP] 2 [/SUP], Saira Ali[SUP] 1 2 [/SUP], Anthony O Fedele[SUP] 1 [/SUP], Michael Z Michael[SUP] 2 3 [/SUP], Darling Rojas-Canales[SUP] 1 2 [/SUP], Jordan Y Li[SUP] 1 2 [/SUP], Chuan Kok Lim[SUP] 4 [/SUP], Jonathan M Gleadle[SUP] 1 2 [/SUP]
Affiliations
- PMID: 38226175
- PMCID: PMC10788221
- DOI: 10.1016/j.isci.2023.108719
Many viruses produce microRNAs (miRNAs), termed viral miRNAs (v-miRNAs), with the capacity to target host gene expression. Bioinformatic and cell culture studies suggest that SARS-CoV-2 can also generate v-miRNAs. This patient-based study defines the SARS-CoV-2 encoded small RNAs present in nasopharyngeal swabs of patients with COVID-19 infection using small RNA-seq. A specific conserved sequence (CoV2-miR-O8) is defined that is not expressed in other coronaviruses but is preserved in all SARS-CoV-2 variants. CoV2-miR-O8 is highly represented in nasopharyngeal samples from patients with COVID-19 infection, is detected by RT-PCR assays in patients, has features consistent with Dicer and Drosha generation as well as interaction with Argonaute and targets specific human microRNAs.
Keywords: Health sciences; Immunology; Medical specialty; Medicine; Virology.