tetano
Editor, Senior Moderator
Jpn J Infect Dis. 2018 Apr 27. doi: 10.7883/yoken.JJID.2017.551. [Epub ahead of print]
[h=1]Isolation of egg-adapted influenza A(H3N2) virus without amino acid substitutions in the antigenic sites of its hemagglutinin.[/h] Kuwahara T[SUP]1[/SUP], Takashita E[SUP]1[/SUP], Fujisaki S[SUP]1[/SUP], Shirakura M[SUP]1[/SUP], Nakamura K[SUP]1[/SUP], Kishida N[SUP]1[/SUP], Takahashi H[SUP]1[/SUP], Suzuki N[SUP]2[/SUP], Kawaoka Y[SUP]3,[/SUP][SUP]4,[/SUP][SUP]5[/SUP], Watanabe S[SUP]1[/SUP], Odagiri T[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Antigenic changes in the hemagglutinin protein of recent A(H3N2) viruses are often induced when these viruses adapt to their egg hosts. We have successfully isolated an egg-adapted influenza A(H3N2) virus, A/Saitama/103/2014, without amino acid substitutions in the antigenic sites of its hemagglutinin protein but multiple substitutions in its neuraminidase protein by serial egg passages of a cell-propagated virus. Antigenic analysis of this egg-adapted A/Saitama/103/2014 virus indicated that its antigenicity did not change from the WHO prototype cell-propagated vaccine virus, A/Hong Kong/4801/2014. Our results suggest that this strategy may facilitate egg-based vaccine production without antigenic alterations in HA by egg adaptation.
[h=4]KEYWORDS:[/h] Influenza A(H3N2) virus; antigenicity; egg-adaptation; hemagglutinin; neuraminidase
PMID: 29709975 DOI: 10.7883/yoken.JJID.2017.551
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[h=1]Isolation of egg-adapted influenza A(H3N2) virus without amino acid substitutions in the antigenic sites of its hemagglutinin.[/h] Kuwahara T[SUP]1[/SUP], Takashita E[SUP]1[/SUP], Fujisaki S[SUP]1[/SUP], Shirakura M[SUP]1[/SUP], Nakamura K[SUP]1[/SUP], Kishida N[SUP]1[/SUP], Takahashi H[SUP]1[/SUP], Suzuki N[SUP]2[/SUP], Kawaoka Y[SUP]3,[/SUP][SUP]4,[/SUP][SUP]5[/SUP], Watanabe S[SUP]1[/SUP], Odagiri T[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Antigenic changes in the hemagglutinin protein of recent A(H3N2) viruses are often induced when these viruses adapt to their egg hosts. We have successfully isolated an egg-adapted influenza A(H3N2) virus, A/Saitama/103/2014, without amino acid substitutions in the antigenic sites of its hemagglutinin protein but multiple substitutions in its neuraminidase protein by serial egg passages of a cell-propagated virus. Antigenic analysis of this egg-adapted A/Saitama/103/2014 virus indicated that its antigenicity did not change from the WHO prototype cell-propagated vaccine virus, A/Hong Kong/4801/2014. Our results suggest that this strategy may facilitate egg-based vaccine production without antigenic alterations in HA by egg adaptation.
[h=4]KEYWORDS:[/h] Influenza A(H3N2) virus; antigenicity; egg-adaptation; hemagglutinin; neuraminidase
PMID: 29709975 DOI: 10.7883/yoken.JJID.2017.551
Free full text