tetano
Editor, Senior Moderator
J Clin Endocrinol Metab
. 2022 Jan 28;dgac038.
doi: 10.1210/clinem/dgac038. Online ahead of print.
Duodenal mucosal expression of COVID-19-related genes in health, diabetes gastroenteropathy and functional dyspepsia
Brototo Deb[SUP] 1 [/SUP], Daniel R O'Brien[SUP] 2 [/SUP], Adil E Bharucha[SUP] 1 [/SUP]
Affiliations
Abstract
Context: SARS-CoV-2 infects the gastrointestinal tract and may be associated with symptoms that resemble diabetic gastroparesis. Why patients with diabetes who contract COVID-19 are more likely to have severe disease is unknown.
Objectives: To compare the duodenal mucosal expression of SARS-CoV-2 and inflammation-related genes in healthy controls, diabetes gastroenteropathy (DGE), and functional dyspepsia (FD).
Design: Gastrointestinal transit, and duodenal mucosal mRNA expression of selected genes were compared in 21 controls, 39 DGE patients, and 37 FD patients. Pathway analyses were performed.
Setting: Tertiary referral center.
Results: Patients had normal, delayed (5 FD [13%] and 13 DGE patients [33%], P=.03 vs controls), or rapid (5 FD[12%] and 5 DGE patients[12%]) gastric emptying. Compared to control participants, 100 SARS-CoV-2-related genes were increased in DGE (FDR<0.05) vs 13 genes in FD; 71 of these 100 genes were differentially expressed in DGE vs FD but only 3 between DGE patients with normal vs delayed GE. Upregulated genes in DGE include the SARS-CoV2 viral entry genes CTSL (|(Fold change) |=1.16; FDR<0.05) and CTSB (|Fold Change|=1.24; FDR<0.05) and selected genes involved in viral replication (eg, EIF2 pathways) and inflammation (CCR2, CXCL2, and LCN2, but not other inflammation-related pathways eg, IL-2 and IL-6 signaling).
Conclusions: Several SARS-CoV-2-related genes were differentially expressed between DGE vs healthy controls and vs FD but not between DGE patients with normal vs delayed GE, which suggests that the differential expression is related to diabetes per se. The upregulation of CTSL and CTSB and replication genes may predispose to SARS-CoV2 infection of the gastrointestinal tract in diabetes.
Keywords: coagulation; coronavirus; diabetes mellitus; idiopathic gastroparesis; vomiting.
. 2022 Jan 28;dgac038.
doi: 10.1210/clinem/dgac038. Online ahead of print.
Duodenal mucosal expression of COVID-19-related genes in health, diabetes gastroenteropathy and functional dyspepsia
Brototo Deb[SUP] 1 [/SUP], Daniel R O'Brien[SUP] 2 [/SUP], Adil E Bharucha[SUP] 1 [/SUP]
Affiliations
- PMID: 35090021
- DOI: 10.1210/clinem/dgac038
Abstract
Context: SARS-CoV-2 infects the gastrointestinal tract and may be associated with symptoms that resemble diabetic gastroparesis. Why patients with diabetes who contract COVID-19 are more likely to have severe disease is unknown.
Objectives: To compare the duodenal mucosal expression of SARS-CoV-2 and inflammation-related genes in healthy controls, diabetes gastroenteropathy (DGE), and functional dyspepsia (FD).
Design: Gastrointestinal transit, and duodenal mucosal mRNA expression of selected genes were compared in 21 controls, 39 DGE patients, and 37 FD patients. Pathway analyses were performed.
Setting: Tertiary referral center.
Results: Patients had normal, delayed (5 FD [13%] and 13 DGE patients [33%], P=.03 vs controls), or rapid (5 FD[12%] and 5 DGE patients[12%]) gastric emptying. Compared to control participants, 100 SARS-CoV-2-related genes were increased in DGE (FDR<0.05) vs 13 genes in FD; 71 of these 100 genes were differentially expressed in DGE vs FD but only 3 between DGE patients with normal vs delayed GE. Upregulated genes in DGE include the SARS-CoV2 viral entry genes CTSL (|(Fold change) |=1.16; FDR<0.05) and CTSB (|Fold Change|=1.24; FDR<0.05) and selected genes involved in viral replication (eg, EIF2 pathways) and inflammation (CCR2, CXCL2, and LCN2, but not other inflammation-related pathways eg, IL-2 and IL-6 signaling).
Conclusions: Several SARS-CoV-2-related genes were differentially expressed between DGE vs healthy controls and vs FD but not between DGE patients with normal vs delayed GE, which suggests that the differential expression is related to diabetes per se. The upregulation of CTSL and CTSB and replication genes may predispose to SARS-CoV2 infection of the gastrointestinal tract in diabetes.
Keywords: coagulation; coronavirus; diabetes mellitus; idiopathic gastroparesis; vomiting.