tetano
Editor, Senior Moderator
J Clin Lipidol
. 2022 Nov 6;S1933-2874(22)00296-3.
doi: 10.1016/j.jacl.2022.10.012. Online ahead of print.
Serum 25-hydroxycholesterol levels are increased in patients with coronavirus disease 2019
Takumi Asano[SUP] 1 [/SUP], Tetsuji Wakabayashi[SUP] 1 [/SUP], Yasuyuki Kondo[SUP] 1 [/SUP], Kenta Okada[SUP] 1 [/SUP], Daisuke Yamamuro[SUP] 1 [/SUP], Yukiko Koga[SUP] 1 [/SUP], Kiyonori Oka[SUP] 1 [/SUP], Momoe Sakurai[SUP] 1 [/SUP], Nagisa Sawayama[SUP] 1 [/SUP], Manabu Takahashi[SUP] 1 [/SUP], Hiroaki Okazaki[SUP] 1 [/SUP], Ken Ebihara[SUP] 1 [/SUP], Kensuke Minami[SUP] 2 [/SUP], Yuji Morisawa[SUP] 2 [/SUP], Shuji Hatakeyama[SUP] 3 [/SUP], Masami Matsumura[SUP] 3 [/SUP], Shun Ishibashi[SUP] 4 [/SUP]
Affiliations
Abstract
Background: 25-hydroxycholesterol (25HC), produced by cholesterol 25-hydroxylase (CH25H) in macrophages, has been reported to inhibit the replication of viral pathogens such as severe acute respiratory syndrome coronavirus-2. Also, CH25H expression in macrophages is robustly induced by interferons (IFNs).
Objective: To better understand the serum level increase of 25HC in coronavirus disease 2019 (COVID-19) and how it relates to the clinical picture.
Methods: We measured the serum levels of 25HC and five other oxysterols in 17 hospitalized COVID-19 patients.
Results: On admission, 25HC and 27-hydroxycholesterol (27HC) serum levels were elevated; however, 7-ketocholesterol (7KC) levels were lower in patients with COVID-19 than in the healthy controls. There was no significant correlation between 25HC serum levels and disease severity markers, such as interferon-gamma (IFN-γ) and interleukin 6. Dexamethasone effectively suppressed cholesterol 25-hydroxylase (CH25H) mRNA expression in RAW 264.7 cells, a murine leukemia macrophage cell line, with or without lipopolysaccharide or IFNs; therefore, it might mitigate the increasing effects of COVID-19 on the serum levels of 25HC.
Conclusions: Our results highlighted that 25HC could be used as a unique biomarker in severe COVID-19 and a potential therapeutic candidate for detecting the severity of COVID-19 and other infectious diseases.
Keywords: 25-Hydroxycholesterol; COVID-19; Cholesterol 25-hydroxylase; Oxysterol; Serum biomarker.
. 2022 Nov 6;S1933-2874(22)00296-3.
doi: 10.1016/j.jacl.2022.10.012. Online ahead of print.
Serum 25-hydroxycholesterol levels are increased in patients with coronavirus disease 2019
Takumi Asano[SUP] 1 [/SUP], Tetsuji Wakabayashi[SUP] 1 [/SUP], Yasuyuki Kondo[SUP] 1 [/SUP], Kenta Okada[SUP] 1 [/SUP], Daisuke Yamamuro[SUP] 1 [/SUP], Yukiko Koga[SUP] 1 [/SUP], Kiyonori Oka[SUP] 1 [/SUP], Momoe Sakurai[SUP] 1 [/SUP], Nagisa Sawayama[SUP] 1 [/SUP], Manabu Takahashi[SUP] 1 [/SUP], Hiroaki Okazaki[SUP] 1 [/SUP], Ken Ebihara[SUP] 1 [/SUP], Kensuke Minami[SUP] 2 [/SUP], Yuji Morisawa[SUP] 2 [/SUP], Shuji Hatakeyama[SUP] 3 [/SUP], Masami Matsumura[SUP] 3 [/SUP], Shun Ishibashi[SUP] 4 [/SUP]
Affiliations
- PMID: 36522261
- DOI: 10.1016/j.jacl.2022.10.012
Abstract
Background: 25-hydroxycholesterol (25HC), produced by cholesterol 25-hydroxylase (CH25H) in macrophages, has been reported to inhibit the replication of viral pathogens such as severe acute respiratory syndrome coronavirus-2. Also, CH25H expression in macrophages is robustly induced by interferons (IFNs).
Objective: To better understand the serum level increase of 25HC in coronavirus disease 2019 (COVID-19) and how it relates to the clinical picture.
Methods: We measured the serum levels of 25HC and five other oxysterols in 17 hospitalized COVID-19 patients.
Results: On admission, 25HC and 27-hydroxycholesterol (27HC) serum levels were elevated; however, 7-ketocholesterol (7KC) levels were lower in patients with COVID-19 than in the healthy controls. There was no significant correlation between 25HC serum levels and disease severity markers, such as interferon-gamma (IFN-γ) and interleukin 6. Dexamethasone effectively suppressed cholesterol 25-hydroxylase (CH25H) mRNA expression in RAW 264.7 cells, a murine leukemia macrophage cell line, with or without lipopolysaccharide or IFNs; therefore, it might mitigate the increasing effects of COVID-19 on the serum levels of 25HC.
Conclusions: Our results highlighted that 25HC could be used as a unique biomarker in severe COVID-19 and a potential therapeutic candidate for detecting the severity of COVID-19 and other infectious diseases.
Keywords: 25-Hydroxycholesterol; COVID-19; Cholesterol 25-hydroxylase; Oxysterol; Serum biomarker.