tetano
Editor, Senior Moderator
J Clin Virol
. 2021 Apr 24;140:104846.
doi: 10.1016/j.jcv.2021.104846. Online ahead of print.
SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious virus
Johan Nordgren[SUP] 1 [/SUP], Sumit Sharma[SUP] 1 [/SUP], Henrik Olsson[SUP] 2 [/SUP], Mikael J?mtberg[SUP] 2 [/SUP], Tina Falkeborn[SUP] 3 [/SUP], Lennart Svensson[SUP] 4 [/SUP], Marie Hagbom[SUP] 5 [/SUP]
Affiliations
Abstract
Background: The COVID-19 pandemic has highlighted the need for rapid, cost effective and easy-to-use diagnostic tools for SARS-CoV-2 infections that can be used in point of care settings to limit disease transmission.
Objective: We evaluated two rapid antigen immunochromatographic tests, Abbott Panbio? COVID-19 Ag Rapid Test (Panbio) and Zhejiang Orient Gene/Healgen Biotech Coronavirus Ag rapid test cassette (Orient gene) for detection of infectious SARS-CoV-2.
Results: The tests were evaluated on nasopharyngeal samples taken from individuals having respiratory and/or COVID-19 related symptoms, which had been analyzed for SARS-CoV-2 RNA using real-time PCR. In total 156 PCR-positive, and 130 (Panbio) and 176 (Orient Gene) PCR-negative samples were analyzed. Overall sensitivity and specificity were 71.8% and 100% for Panbio and 79.5% and 74.4% for the Orient Gene test respectively. The false positives by the Orient Gene test were verified as SARS-CoV-2 negative by in-house real-time PCR assay and were negative for the four seasonal coronaviruses. Subgroup analysis revealed that the antigen tests had high sensitivity for samples with Ct-values <25 (>88%) and for samples containing infectious viruses as determined by cultivation on Vero cells, 94.1% and 97.1% for the Panbio and Orient gene tests, respectively. Furthermore, both tests had a sensitivity of <50 picogram for nucleocapsid protein. No sample with a Ct-value >27 was shown to contain infectious virus.
Conclusion: The results indicate that the rapid antigen tests, especially the Panbio tests may be a valuable tool to detect contagious persons during the ongoing pandemic.
Keywords: Infectious virus; Rapid antigen test; SARS-CoV-2.
. 2021 Apr 24;140:104846.
doi: 10.1016/j.jcv.2021.104846. Online ahead of print.
SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious virus
Johan Nordgren[SUP] 1 [/SUP], Sumit Sharma[SUP] 1 [/SUP], Henrik Olsson[SUP] 2 [/SUP], Mikael J?mtberg[SUP] 2 [/SUP], Tina Falkeborn[SUP] 3 [/SUP], Lennart Svensson[SUP] 4 [/SUP], Marie Hagbom[SUP] 5 [/SUP]
Affiliations
- PMID: 33971580
- DOI: 10.1016/j.jcv.2021.104846
Abstract
Background: The COVID-19 pandemic has highlighted the need for rapid, cost effective and easy-to-use diagnostic tools for SARS-CoV-2 infections that can be used in point of care settings to limit disease transmission.
Objective: We evaluated two rapid antigen immunochromatographic tests, Abbott Panbio? COVID-19 Ag Rapid Test (Panbio) and Zhejiang Orient Gene/Healgen Biotech Coronavirus Ag rapid test cassette (Orient gene) for detection of infectious SARS-CoV-2.
Results: The tests were evaluated on nasopharyngeal samples taken from individuals having respiratory and/or COVID-19 related symptoms, which had been analyzed for SARS-CoV-2 RNA using real-time PCR. In total 156 PCR-positive, and 130 (Panbio) and 176 (Orient Gene) PCR-negative samples were analyzed. Overall sensitivity and specificity were 71.8% and 100% for Panbio and 79.5% and 74.4% for the Orient Gene test respectively. The false positives by the Orient Gene test were verified as SARS-CoV-2 negative by in-house real-time PCR assay and were negative for the four seasonal coronaviruses. Subgroup analysis revealed that the antigen tests had high sensitivity for samples with Ct-values <25 (>88%) and for samples containing infectious viruses as determined by cultivation on Vero cells, 94.1% and 97.1% for the Panbio and Orient gene tests, respectively. Furthermore, both tests had a sensitivity of <50 picogram for nucleocapsid protein. No sample with a Ct-value >27 was shown to contain infectious virus.
Conclusion: The results indicate that the rapid antigen tests, especially the Panbio tests may be a valuable tool to detect contagious persons during the ongoing pandemic.
Keywords: Infectious virus; Rapid antigen test; SARS-CoV-2.