tetano
Editor, Senior Moderator
J Environ Public Health
. 2022 Feb 22;2022:4867626.
doi: 10.1155/2022/4867626. eCollection 2022.
A Simple Method to Detect SARS-CoV-2 in Wastewater at Low Virus Concentration
Supranee Thongpradit[SUP] 1 [/SUP], Somsak Prasongtanakij[SUP] 1 [/SUP], Supanart Srisala[SUP] 1 [/SUP], Yothin Kumsang[SUP] 1 [/SUP], Suwannee Chanprasertyothin[SUP] 1 [/SUP], Pairoj Boonkongchuen[SUP] 2 [/SUP], Dhanesh Pitidhammabhorn[SUP] 2 [/SUP], Parnrudee Manomaipiboon[SUP] 3 [/SUP], Peeraya Somchaiyanon[SUP] 3 [/SUP], Siriwan Chandanachulaka[SUP] 4 [/SUP], Taiyatach Hirunrueng[SUP] 4 [/SUP], Boonsong Ongphiphadhanakul[SUP] 1 5 [/SUP]
Affiliations
Abstract
Background: Since its initial appearance in December 2019, coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread globally. Wastewater surveillance has been demonstrated as capable of identifying infection clusters early. The purpose of this study was to investigate a quick and simple method to detect SARS-CoV-2 in wastewater in Thailand during the early stages of the second outbreak wave when the prevalence of the disease and the virus concentration in wastewater were low.
Methods: Wastewater samples were collected from a hospital caring for patients with COVID-19 and from 35 markets, two of which were associated with recently reported COVID-19 cases. Then, samples were concentrated by membrane filtering prior to SARS-CoV-2 detection by RT-qPCR.
Results: SARS-CoV-2 RNA was detected in the wastewater samples from the hospital; the Ct values for the N, ORF1ab, and S genes progressively increased as the number of patients admitted to the treatment floor decreased. Notably, the ORF1ab and S genes were still detectable in wastewater even when only one patient with COVID-19 remained at the hospital. SARS-CoV-2 RNA was detected in the wastewater samples from fresh market where COVID-19 cases were reported.
Conclusions: Our findings suggest that wastewater surveillance for SARS-CoV-2 is sensitive and can detect the virus even in places with a high ambient temperature and relatively low prevalence of COVID-19.
. 2022 Feb 22;2022:4867626.
doi: 10.1155/2022/4867626. eCollection 2022.
A Simple Method to Detect SARS-CoV-2 in Wastewater at Low Virus Concentration
Supranee Thongpradit[SUP] 1 [/SUP], Somsak Prasongtanakij[SUP] 1 [/SUP], Supanart Srisala[SUP] 1 [/SUP], Yothin Kumsang[SUP] 1 [/SUP], Suwannee Chanprasertyothin[SUP] 1 [/SUP], Pairoj Boonkongchuen[SUP] 2 [/SUP], Dhanesh Pitidhammabhorn[SUP] 2 [/SUP], Parnrudee Manomaipiboon[SUP] 3 [/SUP], Peeraya Somchaiyanon[SUP] 3 [/SUP], Siriwan Chandanachulaka[SUP] 4 [/SUP], Taiyatach Hirunrueng[SUP] 4 [/SUP], Boonsong Ongphiphadhanakul[SUP] 1 5 [/SUP]
Affiliations
- PMID: 35242195
- PMCID: PMC8888108
- DOI: 10.1155/2022/4867626
Abstract
Background: Since its initial appearance in December 2019, coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread globally. Wastewater surveillance has been demonstrated as capable of identifying infection clusters early. The purpose of this study was to investigate a quick and simple method to detect SARS-CoV-2 in wastewater in Thailand during the early stages of the second outbreak wave when the prevalence of the disease and the virus concentration in wastewater were low.
Methods: Wastewater samples were collected from a hospital caring for patients with COVID-19 and from 35 markets, two of which were associated with recently reported COVID-19 cases. Then, samples were concentrated by membrane filtering prior to SARS-CoV-2 detection by RT-qPCR.
Results: SARS-CoV-2 RNA was detected in the wastewater samples from the hospital; the Ct values for the N, ORF1ab, and S genes progressively increased as the number of patients admitted to the treatment floor decreased. Notably, the ORF1ab and S genes were still detectable in wastewater even when only one patient with COVID-19 remained at the hospital. SARS-CoV-2 RNA was detected in the wastewater samples from fresh market where COVID-19 cases were reported.
Conclusions: Our findings suggest that wastewater surveillance for SARS-CoV-2 is sensitive and can detect the virus even in places with a high ambient temperature and relatively low prevalence of COVID-19.