tetano
Editor, Senior Moderator
J Immunol
. 2021 Feb 8;ji2001034.
doi: 10.4049/jimmunol.2001034. Online ahead of print.
T Cell Phenotyping in Individuals Hospitalized with COVID-19
Janine Rupp[SUP] 1 [/SUP], Barbara Dreo[SUP] 1 [/SUP], Katharina G?tl[SUP] 2 [/SUP], Johannes Fessler[SUP] 1 [/SUP], Adrian Moser[SUP] 3 [/SUP], Bernd Haditsch[SUP] 3 [/SUP], Gernot Schilcher[SUP] 4 [/SUP], Lucie-Marie Matzkies[SUP] 5 [/SUP], Ivo Steinmetz[SUP] 5 [/SUP], Hildegard Greinix[SUP] 6 [/SUP], Martin H Stradner[SUP] 7 [/SUP]
Affiliations
Abstract
Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has become pandemic. Cytokine release syndrome occurring in a minority of SARS-CoV-2 infections is associated with severe disease and high mortality. We profiled the composition, activation, and proliferation of T cells in 20 patients with severe or critical COVID-19 and 40 matched healthy controls by flow cytometry. Unsupervised hierarchical cluster analysis based on 18 T cell subsets resulted in separation of healthy controls and COVID-19 patients. Compared to healthy controls, patients suffering from severe and critical COVID-19 had increased frequencies of activated and proliferating CD38[SUP]+[/SUP]Ki67[SUP]+[/SUP] CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T cells, suggesting active antiviral T cell defense. Frequencies of CD38[SUP]+[/SUP]Ki67[SUP]+[/SUP] Th1 and CD4[SUP]+[/SUP] cells correlated negatively with plasma IL-6. Thus, our data suggest that patients suffering from COVID-19 have a distinct T cell composition that is potentially modulated by IL-6.
. 2021 Feb 8;ji2001034.
doi: 10.4049/jimmunol.2001034. Online ahead of print.
T Cell Phenotyping in Individuals Hospitalized with COVID-19
Janine Rupp[SUP] 1 [/SUP], Barbara Dreo[SUP] 1 [/SUP], Katharina G?tl[SUP] 2 [/SUP], Johannes Fessler[SUP] 1 [/SUP], Adrian Moser[SUP] 3 [/SUP], Bernd Haditsch[SUP] 3 [/SUP], Gernot Schilcher[SUP] 4 [/SUP], Lucie-Marie Matzkies[SUP] 5 [/SUP], Ivo Steinmetz[SUP] 5 [/SUP], Hildegard Greinix[SUP] 6 [/SUP], Martin H Stradner[SUP] 7 [/SUP]
Affiliations
- PMID: 33558375
- DOI: 10.4049/jimmunol.2001034
Abstract
Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has become pandemic. Cytokine release syndrome occurring in a minority of SARS-CoV-2 infections is associated with severe disease and high mortality. We profiled the composition, activation, and proliferation of T cells in 20 patients with severe or critical COVID-19 and 40 matched healthy controls by flow cytometry. Unsupervised hierarchical cluster analysis based on 18 T cell subsets resulted in separation of healthy controls and COVID-19 patients. Compared to healthy controls, patients suffering from severe and critical COVID-19 had increased frequencies of activated and proliferating CD38[SUP]+[/SUP]Ki67[SUP]+[/SUP] CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T cells, suggesting active antiviral T cell defense. Frequencies of CD38[SUP]+[/SUP]Ki67[SUP]+[/SUP] Th1 and CD4[SUP]+[/SUP] cells correlated negatively with plasma IL-6. Thus, our data suggest that patients suffering from COVID-19 have a distinct T cell composition that is potentially modulated by IL-6.