tetano
Editor, Senior Moderator
J Infect Chemother
. 2023 Feb 25;S1341-321X(23)00047-8.
doi: 10.1016/j.jiac.2023.02.011. Online ahead of print.
Evaluation of an immunochromatography-based rapid antigen test, Inspecter Kowa SARS-CoV-2, using saliva specimens for the detection of SARS-CoV-2
Masahiro Kodana[SUP] 1 [/SUP], Yuta Orihara[SUP] 2 [/SUP], Mariko Tezuka[SUP] 2 [/SUP], Rina Takahashi[SUP] 2 [/SUP], Sakiko Noguchi[SUP] 2 [/SUP], Nanako Matsuzaki[SUP] 2 [/SUP], Tomohito Takada[SUP] 2 [/SUP], Naomi Kobari[SUP] 2 [/SUP], Kana Ogane[SUP] 2 [/SUP], Rieko Kawamura[SUP] 2 [/SUP], Toru Kawamura[SUP] 2 [/SUP], Shinichi Takeuchi[SUP] 2 [/SUP], Yuki Kamiyama[SUP] 3 [/SUP], Rie Shiomi[SUP] 3 [/SUP], Ryutaro Aoyagi[SUP] 3 [/SUP], Masaya Saito[SUP] 3 [/SUP], Takeru Kusano[SUP] 3 [/SUP], Nobuaki Nakaya[SUP] 3 [/SUP], Satoru Kaneko[SUP] 4 [/SUP], Hideo Morita[SUP] 5 [/SUP], Yoshihito Uchida[SUP] 6 [/SUP], Hiroaki Yazawa[SUP] 7 [/SUP], Ryu Sekiya[SUP] 8 [/SUP], Kazuki Katayama[SUP] 8 [/SUP], Shingo Mikami[SUP] 9 [/SUP], Tomoya Sato[SUP] 10 [/SUP], Norihito Tarumoto[SUP] 11 [/SUP], Takehito Kobayashi[SUP] 3 [/SUP], Hidetomo Nakamoto[SUP] 3 [/SUP], Maeda Takuya[SUP] 2 [/SUP]
Affiliations
Abstract
Background: In the context of the coronavirus disease 2019 (COVID-19) pandemic, a rapid and reliable point-of-care test is an essential tool for controlling the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In particular, an immunochromatography test (ICT) that uses saliva specimens for rapid antigen detection not only reduces the risk of secondary infections but also reduces the burden on medical personnel.
Methods: The newly developed salivary antigen test kit "Inspecter Kowa SARS-CoV-2" is an ICT to which saliva specimens can be directly applied. We evaluated its usefulness in comparison with reverse transcription quantitative PCR (RT-qPCR) and the Espline® SARS-CoV-2 Kit for the detection of SARS-CoV-2 using nasopharyngeal swab specimens. In this study, 140 patients with suspected symptomatic COVID-19 who visited our hospital were enrolled, and nasopharyngeal swab and saliva specimens were collected after they consented to participate in the study.
Results: Inspector Kowa SARS-CoV-2 was positive in 45 of 61 (73.8%) saliva that were positive by RT-qPCR and the Espline® SARS-CoV-2 Kit was also positive in 56 of 60 (93.3%) Np swabs that were positive by RT-qPCR. Good antigen detection was achieved by ICT with saliva and nasopharyngeal swab specimens when viral load was ≥10[SUP]5[/SUP] copies/mL, whereas detection sensitivity was low when viral load was <10[SUP]5[/SUP] copies/mL, especially in saliva specimens.
Conclusion: This ICT for the detection of SARS-CoV-2 salivary antigen is an attractive tool that does not require specialized equipment and allows patients to perform the entire process from sample collection to self-diagnose and to reduce the burden on medical care during a pandemic.
Keywords: Antigen detection; COVID-19; Immunochromatography; SARS-CoV-2; Saliva.
. 2023 Feb 25;S1341-321X(23)00047-8.
doi: 10.1016/j.jiac.2023.02.011. Online ahead of print.
Evaluation of an immunochromatography-based rapid antigen test, Inspecter Kowa SARS-CoV-2, using saliva specimens for the detection of SARS-CoV-2
Masahiro Kodana[SUP] 1 [/SUP], Yuta Orihara[SUP] 2 [/SUP], Mariko Tezuka[SUP] 2 [/SUP], Rina Takahashi[SUP] 2 [/SUP], Sakiko Noguchi[SUP] 2 [/SUP], Nanako Matsuzaki[SUP] 2 [/SUP], Tomohito Takada[SUP] 2 [/SUP], Naomi Kobari[SUP] 2 [/SUP], Kana Ogane[SUP] 2 [/SUP], Rieko Kawamura[SUP] 2 [/SUP], Toru Kawamura[SUP] 2 [/SUP], Shinichi Takeuchi[SUP] 2 [/SUP], Yuki Kamiyama[SUP] 3 [/SUP], Rie Shiomi[SUP] 3 [/SUP], Ryutaro Aoyagi[SUP] 3 [/SUP], Masaya Saito[SUP] 3 [/SUP], Takeru Kusano[SUP] 3 [/SUP], Nobuaki Nakaya[SUP] 3 [/SUP], Satoru Kaneko[SUP] 4 [/SUP], Hideo Morita[SUP] 5 [/SUP], Yoshihito Uchida[SUP] 6 [/SUP], Hiroaki Yazawa[SUP] 7 [/SUP], Ryu Sekiya[SUP] 8 [/SUP], Kazuki Katayama[SUP] 8 [/SUP], Shingo Mikami[SUP] 9 [/SUP], Tomoya Sato[SUP] 10 [/SUP], Norihito Tarumoto[SUP] 11 [/SUP], Takehito Kobayashi[SUP] 3 [/SUP], Hidetomo Nakamoto[SUP] 3 [/SUP], Maeda Takuya[SUP] 2 [/SUP]
Affiliations
- PMID: 36849098
- DOI: 10.1016/j.jiac.2023.02.011
Abstract
Background: In the context of the coronavirus disease 2019 (COVID-19) pandemic, a rapid and reliable point-of-care test is an essential tool for controlling the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In particular, an immunochromatography test (ICT) that uses saliva specimens for rapid antigen detection not only reduces the risk of secondary infections but also reduces the burden on medical personnel.
Methods: The newly developed salivary antigen test kit "Inspecter Kowa SARS-CoV-2" is an ICT to which saliva specimens can be directly applied. We evaluated its usefulness in comparison with reverse transcription quantitative PCR (RT-qPCR) and the Espline® SARS-CoV-2 Kit for the detection of SARS-CoV-2 using nasopharyngeal swab specimens. In this study, 140 patients with suspected symptomatic COVID-19 who visited our hospital were enrolled, and nasopharyngeal swab and saliva specimens were collected after they consented to participate in the study.
Results: Inspector Kowa SARS-CoV-2 was positive in 45 of 61 (73.8%) saliva that were positive by RT-qPCR and the Espline® SARS-CoV-2 Kit was also positive in 56 of 60 (93.3%) Np swabs that were positive by RT-qPCR. Good antigen detection was achieved by ICT with saliva and nasopharyngeal swab specimens when viral load was ≥10[SUP]5[/SUP] copies/mL, whereas detection sensitivity was low when viral load was <10[SUP]5[/SUP] copies/mL, especially in saliva specimens.
Conclusion: This ICT for the detection of SARS-CoV-2 salivary antigen is an attractive tool that does not require specialized equipment and allows patients to perform the entire process from sample collection to self-diagnose and to reduce the burden on medical care during a pandemic.
Keywords: Antigen detection; COVID-19; Immunochromatography; SARS-CoV-2; Saliva.