tetano
Editor, Senior Moderator
J Infect Chemother
. 2021 Feb 25;S1341-321X(21)00065-9.
doi: 10.1016/j.jiac.2021.02.025. Online ahead of print.
Immunochromatography and chemiluminescent enzyme immunoassay for COVID-19 diagnosis
Toshiaki Ishii[SUP] 1 [/SUP], Masakazu Sasaki[SUP] 1 [/SUP], Kageto Yamada[SUP] 2 [/SUP], Daiki Kato[SUP] 3 [/SUP], Hiroyoshi Osuka[SUP] 3 [/SUP], Kotaro Aoki[SUP] 4 [/SUP], Toshisuke Morita[SUP] 5 [/SUP], Yoshikazu Ishii[SUP] 4 [/SUP], Kazuhiro Tateda[SUP] 4 [/SUP]
Affiliations
Abstract
Introduction: The rapid and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required to prevent the spread of COVID-19. This study evaluated the utility of two SARS-CoV-2 antigen detection methods.
Methods: We evaluated two types of antigen detection methods using immunochromatography (Espline) and quantitative chemiluminescent enzyme immunoassay (Lumipulse). RT-PCR was performed as a standard procedure for COVID-19 diagnosis. Lumipulse and RT-PCR were performed for all 486 nasopharyngeal swabs and 136 saliva samples, and the Espline test was performed for 271 nasopharyngeal swabs and 93 saliva samples.
Results: The sensitivity and specificity of the Espline test were 10/11 and 260/260 (100%), respectively for the nasopharyngeal swabs and 3/9 and 84/84 (100%), respectively for the saliva samples. High sensitivities for both saliva (8/9) and nasopharyngeal swabs (22/24) were observed in the Lumipulse test. The specificities of the Lumipulse test for nasopharyngeal swabs and saliva samples were 460/462 (99.6%) and 123/127 (96.9%), respectively.
Conclusion: The Espline test is not effective for saliva samples but is useful for simple and rapid COVID-19 tests using nasopharyngeal swabs because it does not require special devices. The Lumipulse test is a powerful high-throughput tool for COVID-19 diagnosis because it has high detection performance for nasopharyngeal swabs and saliva samples.
Keywords: Antigen detection; Espline; Lumipulse; RT-PCR; SARS-CoV-2.
. 2021 Feb 25;S1341-321X(21)00065-9.
doi: 10.1016/j.jiac.2021.02.025. Online ahead of print.
Immunochromatography and chemiluminescent enzyme immunoassay for COVID-19 diagnosis
Toshiaki Ishii[SUP] 1 [/SUP], Masakazu Sasaki[SUP] 1 [/SUP], Kageto Yamada[SUP] 2 [/SUP], Daiki Kato[SUP] 3 [/SUP], Hiroyoshi Osuka[SUP] 3 [/SUP], Kotaro Aoki[SUP] 4 [/SUP], Toshisuke Morita[SUP] 5 [/SUP], Yoshikazu Ishii[SUP] 4 [/SUP], Kazuhiro Tateda[SUP] 4 [/SUP]
Affiliations
- PMID: 33676843
- DOI: 10.1016/j.jiac.2021.02.025
Abstract
Introduction: The rapid and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required to prevent the spread of COVID-19. This study evaluated the utility of two SARS-CoV-2 antigen detection methods.
Methods: We evaluated two types of antigen detection methods using immunochromatography (Espline) and quantitative chemiluminescent enzyme immunoassay (Lumipulse). RT-PCR was performed as a standard procedure for COVID-19 diagnosis. Lumipulse and RT-PCR were performed for all 486 nasopharyngeal swabs and 136 saliva samples, and the Espline test was performed for 271 nasopharyngeal swabs and 93 saliva samples.
Results: The sensitivity and specificity of the Espline test were 10/11 and 260/260 (100%), respectively for the nasopharyngeal swabs and 3/9 and 84/84 (100%), respectively for the saliva samples. High sensitivities for both saliva (8/9) and nasopharyngeal swabs (22/24) were observed in the Lumipulse test. The specificities of the Lumipulse test for nasopharyngeal swabs and saliva samples were 460/462 (99.6%) and 123/127 (96.9%), respectively.
Conclusion: The Espline test is not effective for saliva samples but is useful for simple and rapid COVID-19 tests using nasopharyngeal swabs because it does not require special devices. The Lumipulse test is a powerful high-throughput tool for COVID-19 diagnosis because it has high detection performance for nasopharyngeal swabs and saliva samples.
Keywords: Antigen detection; Espline; Lumipulse; RT-PCR; SARS-CoV-2.