tetano
Editor, Senior Moderator
J Infect Dev Ctries
. 2022 Mar 31;16(3):445-452.
doi: 10.3855/jidc.15818.
Association of SARS-CoV-2 cycle threshold (Ct) values with clinical course and serum biomarkers in COVID-19 patients
Imran Saglik[SUP] 1 [/SUP], Beyza Ener[SUP] 2 [/SUP], Halis Akalin[SUP] 3 [/SUP], Buşra Ozdemir[SUP] 4 [/SUP], Gokhan Ocakoglu[SUP] 5 [/SUP], Baris Yalcin[SUP] 6 [/SUP], Ugur Onal[SUP] 3 [/SUP], Ozge Aydin Guçlu[SUP] 7 [/SUP], Nilufer Aylin Acet Ozturk[SUP] 7 [/SUP], Ulku Tuzemen[SUP] 2 [/SUP], Ezgi Demirdogen[SUP] 7 [/SUP], Asli Gorek Dilektasli[SUP] 7 [/SUP], Harun Agca[SUP] 2 [/SUP], Esra Kazak[SUP] 3 [/SUP], Funda Coskun[SUP] 7 [/SUP], Yasemin Heper[SUP] 3 [/SUP], Melda Payaslioglu[SUP] 2 [/SUP], Dane Ediger[SUP] 7 [/SUP], Ahmet Ursavas[SUP] 7 [/SUP], Emel Yilmaz[SUP] 3 [/SUP], Cuneyt Ozakin[SUP] 2 [/SUP], Esra Uzaslan[SUP] 7 [/SUP], Mehmet Karadag[SUP] 7 [/SUP]
Affiliations
Abstract
Introduction: Our knowledge has gaps regarding severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication levels and its association to severity of Coronavirus disease 2019 (COVID-19). The aim of this study was to investigate the association of SARS-CoV-2 viral load with disease severity and serum biomarkers in COVID-19 patients.
Methodology: Viral load was determined via cycle threshold (Ct) values of SARS-CoV-2 real-time reverse transcriptase-polymerase chain reaction (RT-PCR) in 214 adult patients. Ct values were compared with clinical severity, biochemical and hematological biomarkers.
Results: Clinical course of the disease was mild (49.1%), moderate (40.2%), and severe (10.7%). Median Ct value was 28.2 (IQR: 22.2-33.8) during the first week of the disease. Ct values were lower within five days after symptom onset [lowest Ct value on the third day (median: 24, IQR: 20.6-32.3)], but they increased significantly during the second and third weeks. No association was detected between admission Ct values and disease severity. Gender, age, co-morbidity, and mortality did not differ significantly in patients with low (≤ 25) and high (> 25) Ct values. White blood cell, neutrophil, platelet, and especially lymphocyte counts, were significantly lower in patients with low Ct values.
Conclusions: No definitive/clear correlation between SARS-CoV-2 viral load and severity and mortality was found in the studied COVID-19 patients. However, neutrophil, platelet, and especially lymphocyte count were significantly lower in patients with a high viral load.
Keywords: COVID-19; Ct value; SARS-CoV-2; lymphocyte; severity.
. 2022 Mar 31;16(3):445-452.
doi: 10.3855/jidc.15818.
Association of SARS-CoV-2 cycle threshold (Ct) values with clinical course and serum biomarkers in COVID-19 patients
Imran Saglik[SUP] 1 [/SUP], Beyza Ener[SUP] 2 [/SUP], Halis Akalin[SUP] 3 [/SUP], Buşra Ozdemir[SUP] 4 [/SUP], Gokhan Ocakoglu[SUP] 5 [/SUP], Baris Yalcin[SUP] 6 [/SUP], Ugur Onal[SUP] 3 [/SUP], Ozge Aydin Guçlu[SUP] 7 [/SUP], Nilufer Aylin Acet Ozturk[SUP] 7 [/SUP], Ulku Tuzemen[SUP] 2 [/SUP], Ezgi Demirdogen[SUP] 7 [/SUP], Asli Gorek Dilektasli[SUP] 7 [/SUP], Harun Agca[SUP] 2 [/SUP], Esra Kazak[SUP] 3 [/SUP], Funda Coskun[SUP] 7 [/SUP], Yasemin Heper[SUP] 3 [/SUP], Melda Payaslioglu[SUP] 2 [/SUP], Dane Ediger[SUP] 7 [/SUP], Ahmet Ursavas[SUP] 7 [/SUP], Emel Yilmaz[SUP] 3 [/SUP], Cuneyt Ozakin[SUP] 2 [/SUP], Esra Uzaslan[SUP] 7 [/SUP], Mehmet Karadag[SUP] 7 [/SUP]
Affiliations
- PMID: 35404849
- DOI: 10.3855/jidc.15818
Abstract
Introduction: Our knowledge has gaps regarding severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication levels and its association to severity of Coronavirus disease 2019 (COVID-19). The aim of this study was to investigate the association of SARS-CoV-2 viral load with disease severity and serum biomarkers in COVID-19 patients.
Methodology: Viral load was determined via cycle threshold (Ct) values of SARS-CoV-2 real-time reverse transcriptase-polymerase chain reaction (RT-PCR) in 214 adult patients. Ct values were compared with clinical severity, biochemical and hematological biomarkers.
Results: Clinical course of the disease was mild (49.1%), moderate (40.2%), and severe (10.7%). Median Ct value was 28.2 (IQR: 22.2-33.8) during the first week of the disease. Ct values were lower within five days after symptom onset [lowest Ct value on the third day (median: 24, IQR: 20.6-32.3)], but they increased significantly during the second and third weeks. No association was detected between admission Ct values and disease severity. Gender, age, co-morbidity, and mortality did not differ significantly in patients with low (≤ 25) and high (> 25) Ct values. White blood cell, neutrophil, platelet, and especially lymphocyte counts, were significantly lower in patients with low Ct values.
Conclusions: No definitive/clear correlation between SARS-CoV-2 viral load and severity and mortality was found in the studied COVID-19 patients. However, neutrophil, platelet, and especially lymphocyte count were significantly lower in patients with a high viral load.
Keywords: COVID-19; Ct value; SARS-CoV-2; lymphocyte; severity.