tetano
Editor, Senior Moderator
J Infect Dis
. 2021 Aug 10;jiab400.
doi: 10.1093/infdis/jiab400. Online ahead of print.
Distinct mitochondria-mediated T cells apoptosis responses in children and adults with COVID-19
Yang Yang[SUP] 1 2 [/SUP], Kuang Liangjian[SUP] 1 [/SUP], Linhai Li[SUP] 2 [/SUP], Wu Yongjian[SUP] 1 2 [/SUP], Bei Zhong[SUP] 2 [/SUP], Xi Huang[SUP] 1 2 [/SUP]
Affiliations
Abstract
Background: Lymphopenia is a key feature for adult patients with coronavirus disease 2019 (COVID-19), while it is rarely observed in children. The underlying mechanism remains unclear.
Methods: Immunohistochemical and flow cytometric analyses were used to compare the apoptotic rate of T cells from COVID-19 adults and children and apoptotic responses of adult and child T cells to COVID-19 pooled plasma. Biological properties of caspases and reactive oxygen species were assessed in T cells treated by COVID-19 pooled plasma.
Results: Mitochondria apoptosis of peripheral T cells were identified in COVID-19 adult patient samples, but not in the children. Furthermore, increased TNF-α and IL-6 in COVID-19 plasma induced mitochondria apoptosis and caused DNA damage by elevating reactive oxygen species levels of the adult T cells. However, the child T cells showed tolerance to mitochondrial apoptosis due to mitochondria autophagy. Activation of autophagy could decrease apoptotic sensitivity of the adult T cells to plasma from COVID-19 patients.
Conclusions: Our results indicated that the mitochondrial apoptosis pathway was activated in T cells of COVID-19 adult patients specifically, which may shed light on the pathophysiological difference between adults and children infected with SARS-CoV-2.
Keywords: COVID-19; adult; children; lymphopenia; mitochondria apoptosis.
. 2021 Aug 10;jiab400.
doi: 10.1093/infdis/jiab400. Online ahead of print.
Distinct mitochondria-mediated T cells apoptosis responses in children and adults with COVID-19
Yang Yang[SUP] 1 2 [/SUP], Kuang Liangjian[SUP] 1 [/SUP], Linhai Li[SUP] 2 [/SUP], Wu Yongjian[SUP] 1 2 [/SUP], Bei Zhong[SUP] 2 [/SUP], Xi Huang[SUP] 1 2 [/SUP]
Affiliations
- PMID: 34374752
- DOI: 10.1093/infdis/jiab400
Abstract
Background: Lymphopenia is a key feature for adult patients with coronavirus disease 2019 (COVID-19), while it is rarely observed in children. The underlying mechanism remains unclear.
Methods: Immunohistochemical and flow cytometric analyses were used to compare the apoptotic rate of T cells from COVID-19 adults and children and apoptotic responses of adult and child T cells to COVID-19 pooled plasma. Biological properties of caspases and reactive oxygen species were assessed in T cells treated by COVID-19 pooled plasma.
Results: Mitochondria apoptosis of peripheral T cells were identified in COVID-19 adult patient samples, but not in the children. Furthermore, increased TNF-α and IL-6 in COVID-19 plasma induced mitochondria apoptosis and caused DNA damage by elevating reactive oxygen species levels of the adult T cells. However, the child T cells showed tolerance to mitochondrial apoptosis due to mitochondria autophagy. Activation of autophagy could decrease apoptotic sensitivity of the adult T cells to plasma from COVID-19 patients.
Conclusions: Our results indicated that the mitochondrial apoptosis pathway was activated in T cells of COVID-19 adult patients specifically, which may shed light on the pathophysiological difference between adults and children infected with SARS-CoV-2.
Keywords: COVID-19; adult; children; lymphopenia; mitochondria apoptosis.