tetano
Editor, Senior Moderator
J Infect Dis
. 2023 Nov 17:jiad458.
doi: 10.1093/infdis/jiad458. Online ahead of print. NLRP12 Senses the SARS-CoV-2 Membrane Protein and Promotes an Inflammatory Response
Xingyu Li[SUP] 1 2 3 [/SUP], Guangde Zhou[SUP] 1 [/SUP], Xingzi Sun[SUP] 1 2 [/SUP], Siying Qu[SUP] 2 [/SUP], Hongzhi Lai[SUP] 4 [/SUP], Yongjian Wu[SUP] 2 [/SUP], Dechang Li[SUP] 5 [/SUP], Lei Liu[SUP] 1 [/SUP], Guoliang Zhang[SUP] 1 [/SUP], Jingwen Yang[SUP] 6 [/SUP], Xi Huang[SUP] 1 2 3 [/SUP]
Affiliations
COVID-19 is an acute respiratory disorder that is caused by SARS-CoV-2, in which excessive systemic inflammation is associated with adverse patient clinical outcomes. Here, we observed elevated expression levels of NLRP12 (nucleotide-binding leucine-rich repeat-containing receptor 12) in human peripheral monocytes and lung tissue during infection with SARS-CoV-2. Co-immunoprecipitation analysis revealed that NLRP12 directly interacted with the M protein through its leucine-rich repeat domain. Moreover, in vitro studies demonstrated that NLRP12 interacted with TRAF3 and promoted its ubiquitination and degradation, which counteracted the inhibitory effect of TRAF3 on the NF-κB/MAPK signaling pathway and promoted the production of inflammatory cytokines. Furthermore, an in vivo study revealed that NLRP12 knockout mice displayed attenuated tissue injury and ameliorated inflammatory responses in the lungs when infected with a SARS-CoV-2 M protein-reconstituted pseudovirus and mouse coronavirus. Taken together, these findings suggest that NLRP12 mediates the inflammatory responses during coronavirus infection.
Keywords: NLRP12; SARS-CoV-2 M protein; TRAF3; inflammation.
. 2023 Nov 17:jiad458.
doi: 10.1093/infdis/jiad458. Online ahead of print. NLRP12 Senses the SARS-CoV-2 Membrane Protein and Promotes an Inflammatory Response
Xingyu Li[SUP] 1 2 3 [/SUP], Guangde Zhou[SUP] 1 [/SUP], Xingzi Sun[SUP] 1 2 [/SUP], Siying Qu[SUP] 2 [/SUP], Hongzhi Lai[SUP] 4 [/SUP], Yongjian Wu[SUP] 2 [/SUP], Dechang Li[SUP] 5 [/SUP], Lei Liu[SUP] 1 [/SUP], Guoliang Zhang[SUP] 1 [/SUP], Jingwen Yang[SUP] 6 [/SUP], Xi Huang[SUP] 1 2 3 [/SUP]
Affiliations
- PMID: 37976229
- DOI: 10.1093/infdis/jiad458
COVID-19 is an acute respiratory disorder that is caused by SARS-CoV-2, in which excessive systemic inflammation is associated with adverse patient clinical outcomes. Here, we observed elevated expression levels of NLRP12 (nucleotide-binding leucine-rich repeat-containing receptor 12) in human peripheral monocytes and lung tissue during infection with SARS-CoV-2. Co-immunoprecipitation analysis revealed that NLRP12 directly interacted with the M protein through its leucine-rich repeat domain. Moreover, in vitro studies demonstrated that NLRP12 interacted with TRAF3 and promoted its ubiquitination and degradation, which counteracted the inhibitory effect of TRAF3 on the NF-κB/MAPK signaling pathway and promoted the production of inflammatory cytokines. Furthermore, an in vivo study revealed that NLRP12 knockout mice displayed attenuated tissue injury and ameliorated inflammatory responses in the lungs when infected with a SARS-CoV-2 M protein-reconstituted pseudovirus and mouse coronavirus. Taken together, these findings suggest that NLRP12 mediates the inflammatory responses during coronavirus infection.
Keywords: NLRP12; SARS-CoV-2 M protein; TRAF3; inflammation.