tetano
Editor, Senior Moderator
J Infect Dis
. 2021 Aug 16;224(4):616-619.
doi: 10.1093/infdis/jiab278.
Sensitive Immunodetection of Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern 501Y.V2 and 501Y.V1
Moria Barlev-Gross[SUP] 1 [/SUP], Shay Weiss[SUP] 1 [/SUP], Nir Paran[SUP] 1 [/SUP], Yfat Yahalom-Ronen[SUP] 1 [/SUP], Ofir Israeli[SUP] 2 [/SUP], Ital Nemet[SUP] 3 [/SUP], Limor Kliker[SUP] 3 [/SUP], Neta Zuckerman[SUP] 3 [/SUP], Itai Glinert[SUP] 1 [/SUP], Tal Noy-Porat[SUP] 2 [/SUP], Ron Alcalay[SUP] 2 [/SUP], Ronit Rosenfeld[SUP] 2 [/SUP], Haim Levy[SUP] 1 [/SUP], Ohad Mazor[SUP] 1 [/SUP], Michal Mandelboim[SUP] 3 4 [/SUP], Ella Mendelson[SUP] 3 4 [/SUP], Adi Beth-Din[SUP] 2 [/SUP], Tomer Israely[SUP] 1 [/SUP], Adva Mechaly[SUP] 1 [/SUP]
Affiliations
Abstract
Emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants may influence the effectiveness of existing laboratory diagnostics. In the current study we determined whether the British (20I/501Y.V1) and South African (20H/501Y.V2) SARS-CoV-2 variants of concern are detected with an in-house S1-based antigen detection assay, analyzing spiked pools of quantitative reverse-transcription polymerase chain reaction-negative nasopharyngeal swab specimens. The assay, combining 4 monoclonal antibodies, allowed sensitive detection of both the wild type and the variants of concern, despite accumulation of several mutations in the variants' S1 region-results suggesting that this combination, targeting distinct epitopes, enables both specificity and the universality.
Keywords: 501Y.V1; 501Y.V2; Antibodies; Antigen detection; SARS-CoV-2; Variants of Concern; spike protein.
. 2021 Aug 16;224(4):616-619.
doi: 10.1093/infdis/jiab278.
Sensitive Immunodetection of Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern 501Y.V2 and 501Y.V1
Moria Barlev-Gross[SUP] 1 [/SUP], Shay Weiss[SUP] 1 [/SUP], Nir Paran[SUP] 1 [/SUP], Yfat Yahalom-Ronen[SUP] 1 [/SUP], Ofir Israeli[SUP] 2 [/SUP], Ital Nemet[SUP] 3 [/SUP], Limor Kliker[SUP] 3 [/SUP], Neta Zuckerman[SUP] 3 [/SUP], Itai Glinert[SUP] 1 [/SUP], Tal Noy-Porat[SUP] 2 [/SUP], Ron Alcalay[SUP] 2 [/SUP], Ronit Rosenfeld[SUP] 2 [/SUP], Haim Levy[SUP] 1 [/SUP], Ohad Mazor[SUP] 1 [/SUP], Michal Mandelboim[SUP] 3 4 [/SUP], Ella Mendelson[SUP] 3 4 [/SUP], Adi Beth-Din[SUP] 2 [/SUP], Tomer Israely[SUP] 1 [/SUP], Adva Mechaly[SUP] 1 [/SUP]
Affiliations
- PMID: 34398244
- DOI: 10.1093/infdis/jiab278
Abstract
Emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants may influence the effectiveness of existing laboratory diagnostics. In the current study we determined whether the British (20I/501Y.V1) and South African (20H/501Y.V2) SARS-CoV-2 variants of concern are detected with an in-house S1-based antigen detection assay, analyzing spiked pools of quantitative reverse-transcription polymerase chain reaction-negative nasopharyngeal swab specimens. The assay, combining 4 monoclonal antibodies, allowed sensitive detection of both the wild type and the variants of concern, despite accumulation of several mutations in the variants' S1 region-results suggesting that this combination, targeting distinct epitopes, enables both specificity and the universality.
Keywords: 501Y.V1; 501Y.V2; Antibodies; Antigen detection; SARS-CoV-2; Variants of Concern; spike protein.