tetano
Editor, Senior Moderator
J Med Virol
. 2020 Jul 7.
doi: 10.1002/jmv.26261. Online ahead of print.
An Update on Origin of SARS-CoV-2: Despite Closest Identity, Bat (RaTG13) and Pangolin Derived Coronaviruses Varied in the Critical Binding Site and O-linked Glycan Residues
Jeevan Malaiyan[SUP] 1 [/SUP], Suresh Arumugam[SUP] 2 [/SUP], Kamalraj Mohan[SUP] 1 [/SUP], Gokul Gomathi Radhakrishnan[SUP] 3 [/SUP]
Affiliations
Abstract
The initial cases of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) occurred in Wuhan, China, in December 2019 and swept the world by 23 June 2020 with 8,993,659 active cases, 469,587 deaths across 216 countries, areas or territories. This strongly implies global transmission occurred before the lockdown of China. However, the initial source's transmission routes of SARS-CoV-2 remain obscure and controversial. Research data suggest bat (RaTG13) and pangolin carried CoV were the proximal source of SARS-CoV-2. In this study, we used systematic phylogenetic analysis of Coronavirinae subfamily along with wild type human SARS-CoV, MERS-CoV, and SARS-CoV-2 strains. The key residues of the receptor-binding domain (RBD) and O-linked glycan were compared. SARS-CoV-2 strains were clustered with RaTG13 (97.41% identity), Pangolin-CoV (92.22% identity) and Bat-SL-CoV (80.36% identity), forms a new clade-2 in lineage B of beta-CoV. The alignments of RBD contact residues to ACE2 justified? Those SARS-CoV-2 strains sequences were 100% identical by each other, significantly varied in RaTG13 and pangolin-CoV. SARS-CoV-2 has a polybasic cleavage site with an inserted sequence of PRRA compared to RaTG13 and only PRR to pangolin. Only serine (Ser) in pangolin and both threonine (Thr) and serine (Ser) O-linked glycans were seen in RaTG13, suggesting that a detailed study needed in Pangolin (Manis javanica) and bat (Rhinolophus affinis) related CoV. This article is protected by copyright. All rights reserved.
Keywords: COVID-19; RaTg13; SARS-CoV-2; intermediate host; pangolins.
. 2020 Jul 7.
doi: 10.1002/jmv.26261. Online ahead of print.
An Update on Origin of SARS-CoV-2: Despite Closest Identity, Bat (RaTG13) and Pangolin Derived Coronaviruses Varied in the Critical Binding Site and O-linked Glycan Residues
Jeevan Malaiyan[SUP] 1 [/SUP], Suresh Arumugam[SUP] 2 [/SUP], Kamalraj Mohan[SUP] 1 [/SUP], Gokul Gomathi Radhakrishnan[SUP] 3 [/SUP]
Affiliations
- PMID: 32633815
- DOI: 10.1002/jmv.26261
Abstract
The initial cases of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) occurred in Wuhan, China, in December 2019 and swept the world by 23 June 2020 with 8,993,659 active cases, 469,587 deaths across 216 countries, areas or territories. This strongly implies global transmission occurred before the lockdown of China. However, the initial source's transmission routes of SARS-CoV-2 remain obscure and controversial. Research data suggest bat (RaTG13) and pangolin carried CoV were the proximal source of SARS-CoV-2. In this study, we used systematic phylogenetic analysis of Coronavirinae subfamily along with wild type human SARS-CoV, MERS-CoV, and SARS-CoV-2 strains. The key residues of the receptor-binding domain (RBD) and O-linked glycan were compared. SARS-CoV-2 strains were clustered with RaTG13 (97.41% identity), Pangolin-CoV (92.22% identity) and Bat-SL-CoV (80.36% identity), forms a new clade-2 in lineage B of beta-CoV. The alignments of RBD contact residues to ACE2 justified? Those SARS-CoV-2 strains sequences were 100% identical by each other, significantly varied in RaTG13 and pangolin-CoV. SARS-CoV-2 has a polybasic cleavage site with an inserted sequence of PRRA compared to RaTG13 and only PRR to pangolin. Only serine (Ser) in pangolin and both threonine (Thr) and serine (Ser) O-linked glycans were seen in RaTG13, suggesting that a detailed study needed in Pangolin (Manis javanica) and bat (Rhinolophus affinis) related CoV. This article is protected by copyright. All rights reserved.
Keywords: COVID-19; RaTg13; SARS-CoV-2; intermediate host; pangolins.