tetano
Editor, Senior Moderator
J Med Virol
. 2022 Mar 8.
doi: 10.1002/jmv.27704. Online ahead of print.
ANALYSIS OF MITOCHONDRIAL DNA CYTOCHROME-B (CYB) and ATPase-6 GENE MUTATIONS IN COVID-19 PATIENTS
Ebubekir Dirican[SUP] 1 [/SUP], Şeyda Tuba Savrun[SUP] 2 [/SUP], İsmail Erkan Aydın[SUP] 2 [/SUP], Gonca Gülbay[SUP] 3 [/SUP], Ülkü Karaman[SUP] 4 [/SUP]
Affiliations
Abstract
Background: Coronavirus disease of 2019 (COVID-19) is a pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Mutations of mitochondrial DNA (mtDNA) are becoming increasingly common in various diseases. This study aims to investigate mutations in the cytochrome-b (CYB) and adenosine triphosphatase-6 (ATPase-6) genes of mtDNA in COVID-19 patients. The association between mtDNA mutations and clinical outcomes is investigated.
Methods: In the present study, mutations of the mtDNA genes CYB and ATPase-6 were investigated in COVID-19(+) (n=65) and COVID-19 (-) patients (n=65). First, we isolated DNA from the blood samples. After the PCR analyses, the mutations were defined using Sanger DNA sequencing.
Results: The age, creatinine, ferritin and CRP levels of the COVID 19 (+) patients were higher than those of the COVID-19 (-) patients (p=0.0036, p=0.0383, p=0.0305, p<0.0001, respectively). We also found 16 different mutations in the CYB gene and 14 different mutations in the ATPase-6 gene. The incidences of CYB gene mutations A15326G, T15454C and C15452A were higher in COVID-19 (+) patients than COVID-19 (-) patients (p<0.0001 (OR (95% CI): 4.966 (2.215-10.89)), p=0.0226 and p=0.0226, respectively). In contrast, the incidences of A8860G and G9055A ATPase-6 gene mutations were higher in COVID-19 (+) patients than COVID-19 (-) patients (p<0.0001 (OR (95%CI): 5.333 (2.359-12.16) and p=0.0121 respectively). Yet, no significant relationship was found between mtDNA mutations and patients' age and biochemical parameters (p> 0.05).
Conclusions: The results showed that the frequency of mtDNA mutations in COVID-19 patients is quite high and it is important to investigate the association of these mutations with other genetic mechanisms in larger patient populations. This article is protected by copyright. All rights reserved.
Keywords: ATPase-6; COVID-19; CYB; PCR; Sanger sequencing; mtDNA.
. 2022 Mar 8.
doi: 10.1002/jmv.27704. Online ahead of print.
ANALYSIS OF MITOCHONDRIAL DNA CYTOCHROME-B (CYB) and ATPase-6 GENE MUTATIONS IN COVID-19 PATIENTS
Ebubekir Dirican[SUP] 1 [/SUP], Şeyda Tuba Savrun[SUP] 2 [/SUP], İsmail Erkan Aydın[SUP] 2 [/SUP], Gonca Gülbay[SUP] 3 [/SUP], Ülkü Karaman[SUP] 4 [/SUP]
Affiliations
- PMID: 35258110
- DOI: 10.1002/jmv.27704
Abstract
Background: Coronavirus disease of 2019 (COVID-19) is a pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Mutations of mitochondrial DNA (mtDNA) are becoming increasingly common in various diseases. This study aims to investigate mutations in the cytochrome-b (CYB) and adenosine triphosphatase-6 (ATPase-6) genes of mtDNA in COVID-19 patients. The association between mtDNA mutations and clinical outcomes is investigated.
Methods: In the present study, mutations of the mtDNA genes CYB and ATPase-6 were investigated in COVID-19(+) (n=65) and COVID-19 (-) patients (n=65). First, we isolated DNA from the blood samples. After the PCR analyses, the mutations were defined using Sanger DNA sequencing.
Results: The age, creatinine, ferritin and CRP levels of the COVID 19 (+) patients were higher than those of the COVID-19 (-) patients (p=0.0036, p=0.0383, p=0.0305, p<0.0001, respectively). We also found 16 different mutations in the CYB gene and 14 different mutations in the ATPase-6 gene. The incidences of CYB gene mutations A15326G, T15454C and C15452A were higher in COVID-19 (+) patients than COVID-19 (-) patients (p<0.0001 (OR (95% CI): 4.966 (2.215-10.89)), p=0.0226 and p=0.0226, respectively). In contrast, the incidences of A8860G and G9055A ATPase-6 gene mutations were higher in COVID-19 (+) patients than COVID-19 (-) patients (p<0.0001 (OR (95%CI): 5.333 (2.359-12.16) and p=0.0121 respectively). Yet, no significant relationship was found between mtDNA mutations and patients' age and biochemical parameters (p> 0.05).
Conclusions: The results showed that the frequency of mtDNA mutations in COVID-19 patients is quite high and it is important to investigate the association of these mutations with other genetic mechanisms in larger patient populations. This article is protected by copyright. All rights reserved.
Keywords: ATPase-6; COVID-19; CYB; PCR; Sanger sequencing; mtDNA.