tetano
Editor, Senior Moderator
J Med Virol
. 2022 May 10.
doi: 10.1002/jmv.27842. Online ahead of print.
Detection of SARS-CoV-2 in Different Human Biofluids Using the Loop-Mediated Isothermal Amplification Assay: A Prospective Diagnostic Study in Fortaleza, Brazil
Marco Clementino[SUP] 1 [/SUP], Karene Ferreira Cavalcante[SUP] 2 [/SUP], Vania Angelica Feitosa Viana[SUP] 2 [/SUP], Dayara de Oliveira Silva[SUP] 1 [/SUP], Caroline Rebouças Damasceno[SUP] 1 [/SUP], Jessica Fernandes de Souza[SUP] 1 [/SUP], Rafhaella Nogueira Della Guardia Gondim[SUP] 1 [/SUP], Daniel Macedo de Melo Jorge[SUP] 3 [/SUP], Lyvia Maria Vasconcelos Carneiro Magalhães[SUP] 1 [/SUP], Érico Antônio Gomes de Arruda[SUP] 4 [/SUP], Roberto da Justa Pires Neto[SUP] 4 [/SUP], Melissa Soares Medeiros[SUP] 4 [/SUP], Armênio Aguiar Dos Santos[SUP] 1 [/SUP], Pedro Jorge Caldas Magalhães[SUP] 1 [/SUP], Liana Perdigão Mello[SUP] 2 [/SUP], Eurico Arruda[SUP] 3 5 [/SUP], Aldo Ângelo Moreira Lima[SUP] 1 5 [/SUP], Alexandre Havt[SUP] 1 [/SUP]
Affiliations
Abstract
We adopted the reverse transcriptase - loop mediated isothermal amplification (RT-LAMP) to detect SARS-Cov-2 in patient samples. Two primer sets for genes N and Orf1ab were designed to detect SARS-CoV-2, and one primer set was designed to detect the human gene Actin. We collected prospective 138 nasopharyngeal swabs, 70 oropharyngeal swabs, 69 saliva, and 68 mouth saline wash samples from patients suspected to have severe acute respiratory syndrome (SARS) caused by SARS-CoV-2 to test the RT-LAMP in comparison with the gold standard technique RT-qPCR. Accuracy of diagnosis using both primers, N5 and Orf9, was evaluated. Sensitivity and specificity for diagnosis was 96% (95% CI 87-99) and 85% (95% CI 76-91) in 138 samples, respectively. Accurate diagnosis results were obtained only in nasopharyngeal swab processed via extraction kit. Accurate and rapid diagnosis could aid COVID-19 pandemic management by identifying, isolating, and treating patients rapidly. This article is protected by copyright. All rights reserved.
Keywords: Coronavirus; Molecular Diagnosis; RT-LAMP.
. 2022 May 10.
doi: 10.1002/jmv.27842. Online ahead of print.
Detection of SARS-CoV-2 in Different Human Biofluids Using the Loop-Mediated Isothermal Amplification Assay: A Prospective Diagnostic Study in Fortaleza, Brazil
Marco Clementino[SUP] 1 [/SUP], Karene Ferreira Cavalcante[SUP] 2 [/SUP], Vania Angelica Feitosa Viana[SUP] 2 [/SUP], Dayara de Oliveira Silva[SUP] 1 [/SUP], Caroline Rebouças Damasceno[SUP] 1 [/SUP], Jessica Fernandes de Souza[SUP] 1 [/SUP], Rafhaella Nogueira Della Guardia Gondim[SUP] 1 [/SUP], Daniel Macedo de Melo Jorge[SUP] 3 [/SUP], Lyvia Maria Vasconcelos Carneiro Magalhães[SUP] 1 [/SUP], Érico Antônio Gomes de Arruda[SUP] 4 [/SUP], Roberto da Justa Pires Neto[SUP] 4 [/SUP], Melissa Soares Medeiros[SUP] 4 [/SUP], Armênio Aguiar Dos Santos[SUP] 1 [/SUP], Pedro Jorge Caldas Magalhães[SUP] 1 [/SUP], Liana Perdigão Mello[SUP] 2 [/SUP], Eurico Arruda[SUP] 3 5 [/SUP], Aldo Ângelo Moreira Lima[SUP] 1 5 [/SUP], Alexandre Havt[SUP] 1 [/SUP]
Affiliations
- PMID: 35535440
- DOI: 10.1002/jmv.27842
Abstract
We adopted the reverse transcriptase - loop mediated isothermal amplification (RT-LAMP) to detect SARS-Cov-2 in patient samples. Two primer sets for genes N and Orf1ab were designed to detect SARS-CoV-2, and one primer set was designed to detect the human gene Actin. We collected prospective 138 nasopharyngeal swabs, 70 oropharyngeal swabs, 69 saliva, and 68 mouth saline wash samples from patients suspected to have severe acute respiratory syndrome (SARS) caused by SARS-CoV-2 to test the RT-LAMP in comparison with the gold standard technique RT-qPCR. Accuracy of diagnosis using both primers, N5 and Orf9, was evaluated. Sensitivity and specificity for diagnosis was 96% (95% CI 87-99) and 85% (95% CI 76-91) in 138 samples, respectively. Accurate diagnosis results were obtained only in nasopharyngeal swab processed via extraction kit. Accurate and rapid diagnosis could aid COVID-19 pandemic management by identifying, isolating, and treating patients rapidly. This article is protected by copyright. All rights reserved.
Keywords: Coronavirus; Molecular Diagnosis; RT-LAMP.